Conserved Protein Domain Family
DNA_pol_III_epsilon_like

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cd06130: DNA_pol_III_epsilon_like 
an uncharacterized bacterial subgroup of the DEDDh 3'-5' exonuclease domain family with similarity to the epsilon subunit of DNA polymerase III
This subfamily is composed of uncharacterized bacterial proteins with similarity to the epsilon subunit of DNA polymerase III (Pol III), a multisubunit polymerase which is the main DNA replicating enzyme in bacteria, functioning as the chromosomal replicase. The Pol III holoenzyme is a complex of ten different subunits, three of which (alpha, epsilon, and theta) compose the catalytic core. The Pol III epsilon subunit, encoded by the dnaQ gene, is a DEDDh-type 3'-5' exonuclease which is responsible for the proofreading activity of the polymerase, increasing the fidelity of DNA synthesis. It contains three conserved sequence motifs termed ExoI, ExoII and ExoIII, with a specific Hx(4)D conserved pattern at ExoIII. These motifs are clustered around the active site and contain four conserved acidic residues that serve as ligands for the two metal ions required for catalysis. The epsilon subunit of Pol III also functions as a stabilizer of the holoenzyme complex.
Statistics
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PSSM-Id: 99834
View PSSM: cd06130
Aligned: 55 rows
Threshold Bit Score: 186.563
Threshold Setting Gi: 126725765
Created: 3-Mar-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            #### #                                                                      
YP_001516725  10 FVAIDFETADYqrDSACAIGVIRVENDQIVDRSHFLIRPP--RQKFQFTYIHGITWSDVKHHPTFAELWPNLQPKFAGsD 87  Acaryochloris m...
ZP_02033116    4 FAAIDFEIANGkrSSICSIGVAIIEDNKVIDSVYMLVRPYpnFYTRFTTAIHGITMQDTIDSPDFEEAWTRIAGRLQD-I 82  Parabacteroides...
NP_904554      4 FAAIDFETANAerTSVCSVGIIVVRGGQIVHRYYSLIRPEpdYYNYHNTRVHGLTAADTESARIFPDVWAEVEPLIAG-L 82  Porphyromonas g...
ZP_01876597    3 FVAIDFETATAkrNSACAVAIITVEKGVIVDEYSELIQPPnnEYSWRNIDVHGIEPRHTMYKEDFEDLYPEIRKRLTG-K 81  Lentisphaera ar...
ZP_01085720   26 FVALDFETANYsrDSVCSLAIIRVESLKVVSRTYRLIRPP--SPHFMFTDIHGITWGDVKSEPTFKELWHEFRPLFEGiS 103 Synechococcus s...
CAM75606       3 FIALDFETADNgrDSACSLALAVVKNGTVAETWHRLIRPP--RASQMFTHIHGITWDMVKDQPDFGHFWPEIRDLIDAaP 80  Magnetospirillu...
ZP_01290732    4 FAAIDFETANYrrESACAVGVVLVEQGRIARRIYHLIRPP--SSWFVFTHIHGLGWEDVRRAPSFGEIWPEIAAELAGvE 81  delta proteobac...
YP_676643      3 FTALDFETANThkTSICAVGMAIVEDGKVVKTISKLIKPVpdFYSYWNVQIHGITPDMTADAPYFIDVWQELKQYIDY-S 81  Cytophaga hutch...
ZP_02734849   18 FVAIDFETADHgpDSACAIGLARVENNKVVRREAVLIRPP--RPRVMFTEVHGITWPMVQNASPFGDVWRGLLPIIEGaK 95  Gemmata obscuri...
YP_001296089   3 FTAIDFETATA--FHPCSVGIVTVQNGVIVDEFVTLIKPPnnQYSPFTIAVHGIRPRDTVNAKSFLEVYPEIKKRLQN-K 79  Flavobacterium ...
Feature 1            ## ###                                                         #    #       
YP_001516725  88 FVAAHNaNFDRSVLNACCQAAELDPM----PLPFLCTVKLAREVWNi-RPTKLPNVCEFLDIPLQ-HHQALSDAEACAKI 161 Acaryochloris m...
ZP_02033116   83 PLVAHNsSFDEGCLRAAHEAYDLAYP----KYQFYCTCRLSRRMYPflANHQLQTVAAHCGYDLTyHHHAMADAYACAHI 158 Parabacteroides...
NP_904554     83 PLVAHNkPFDEGCLKAVFRMYRMDYP----DYPFFCTLQAARRQLRhlSDHRLPTVAQCCGYTLAqHHQALADAEACAAI 158 Porphyromonas g...
ZP_01876597   82 LVVAHNeSFDRSVMTRSMEYYDLDYAdlgvADRWVCTVKLYKAQGF--KPAKLNILCDHFGIPLK-HHDALSDARACAEL 158 Lentisphaera ar...
ZP_01085720  104 FIAAHNaSFDSSVMRACCDTYRIVPP----GAPYLCTVKLARQTWQl-RPANLPSVCAYLGLHLE-HHNALSDAEACANI 177 Synechococcus s...
CAM75606      81 FLAAHNaPFDKGVLAECCRAHGHGAP----LTPFVCTVRVARDTWNl-RPTKLPDVCRFLAQPLN-HHDALSDAVACAEI 154 Magnetospirillu...
ZP_01290732   82 FLAAHNaSFDRGVLNACCHAHGLAPP----SLPFVCTVQLARQRWNi-RPTKLSDVCRRLDIALN-HHQADSDAEACARI 155 delta proteobac...
YP_676643     82 TLVAHNaAFDVGALRSILTTHEVSFP----KTDYFCSCSVSRKVFKeiENHKLSTVCRHIDVPLN-HHEAESDAIGAAQI 156 Cytophaga hutch...
ZP_02734849   96 FLAAHNaSFDRRVLHACCIAAGLDVP----ALPFVCTVQIARRKWAl-KPANLPAVCRRLGIGLI-HHHAGSDAEACARI 169 Gemmata obscuri...
YP_001296089  80 TVVAHNeSFDRNVLAKSMALYGLDYEtlniSNRWECTLKIYKAKGF--KPTKLSDCCQEMNIKLN-HHEALSDARACAKL 156 Flavobacterium ...
Feature 1           
YP_001516725 162 VIA 164 Acaryochloris marina MBIC11017
ZP_02033116  159 AAT 161 Parabacteroides merdae ATCC 43184
NP_904554    159 ALK 161 Porphyromonas gingivalis W83
ZP_01876597  159 YKR 161 Lentisphaera araneosa HTCC2155
ZP_01085720  178 VRA 180 Synechococcus sp. WH 5701
CAM75606     155 MLA 157 Magnetospirillum gryphiswaldense MSR-1
ZP_01290732  156 VIA 158 delta proteobacterium MLMS-1
YP_676643    157 IIK 159 Cytophaga hutchinsonii ATCC 33406
ZP_02734849  170 ILA 172 Gemmata obscuriglobus UQM 2246
YP_001296089 157 YLL 159 Flavobacterium psychrophilum JIP02/86

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