1NXG,1K3P,2H12


Conserved Protein Domain Family
EcCS_like

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cd06114: EcCS_like 
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Escherichia coli (Ec) citrate synthase (CS) GltA_like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs including EcCS are strongly and specifically inhibited by NADH through an allosteric mechanism. Included in this group is an NADH-insensitive type II Acetobacter acetii CS which has retained many of the residues used by EcCS for NADH binding.
Statistics
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PSSM-Id: 99867
View PSSM: cd06114
Aligned: 33 rows
Threshold Bit Score: 666.206
Threshold Setting Gi: 15553143
Created: 1-Nov-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                             #                                                
1NXG_A              18 LDVLKGTLGQDVIDIRTLgs-kGVFTFDPGFTSTASCESKITFIDGdeGILLHRGFPIDQLATDSn-YLEVCYILLNGEK 95  Escherich...
2H12_A              24 MPVLSGTLGPDVIDIRKLpaqlGVFTFDPGYGETAACNSKITFIDGdkGVLLHRGYPIAQLAENAs-YEEVIYLLLNGEL 102 Acetobact...
NP_298824           18 LPVLKPTLGNGCVDIAKLtketGLFTYDSGFTATASCKSAITYIDGekGVLLYRGYPIEQLAEHSs-FLEVAYLLMNGEL 96  Xylella f...
XP_001433892        46 LPVYKGTKGPPMIDITQLysktGYFTLDPGFSSTGACMSTMTYIDGdkGELLYRGYPIETLAKYSs-YIEVCYLFIYGHL 124 Parameciu...
YP_996181           24 FPVLTPTNGPEMLDIGDLhkstGVCTLDIGLMNTGICKSAITYVNGdeGILRYRGYSIDDLVDNCd-YLDVAWLLLNGEL 102 Vermineph...
EDN36925            23 LPVYSPSLGNDCIDVSSLvk-hGIFTYDPGFMSTAACESKITYIDGgkGVLLHRGYPIEEWTQKSn-YRTLCYALIYGEL 100 Francisel...
YP_001359688        19 YDIMDGTRGPSVVDLRSFfadtGMFTYDPGYTSTASCSSQITFIDGakGELRYRGIPIEKLATEHs-YLETCYLLLNGEL 97  Sulfurovu...
ABF58102            16 LPILYDTEGNEVLDITSLykntGVLTYDPGFMSTAACESKITFIDGnkGILRYRGYDIADLVYNNksFIEVVYLLFYGVF 95  heartwate...
REF_mpdneb:Wbm0735  17 LPVLSGTMGYDVLNIKDLykvtGLLTYDPGFVSTASCSSAITFIDGdeGVLRYRGHDIADLAQSNd-FTAVIYLLLYGKL 95  Wolbachia...
AAL01655            16 LPVLRGTDGTPVVDITTLyrenNVLTYDPGFMSTAACRSEITFIDGnkGTLRYRGIDIENLIGTPnsFSSIVYLLLNGTL 95  agent of ...
Feature 1                                                                                              
1NXG_A              96 PTQEQYDEFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYhdsldvnnprhrEIAAFRLLSKMPTMAAM 175 Escherich...
2H12_A             103 PNKAQYDTFTNTLTNHTLLHEQIRNFFNGFRRDAHPMAILCGTVGALSAFYpdandiaipanrDLAAMRLIAKIPTIAAW 182 Acetobact...
NP_298824           97 PRKDEFAKFDDEITHHTMMHESLKNFLGGFHYDAHPMAMLAASVASLSAFYhdtldlndleqrRLAAIRLIAKVPTLAAA 176 Xylella f...
XP_001433892       125 PSQKELQLFEETMVSEMMINEKLIEFYKGFASDSHPMAIMVGVVGALSAFMhkdfdvndprdrEYIAIKLVAKMPTLAAY 204 Parameciu...
YP_996181          103 PTPSQKAEFQYNITYHSMVNEQMAYFLRGFRRDAHPMAVMCGMTGALSAFYhdeldifnaehrKIVAHRLIAKMPTIAAM 182 Vermineph...
EDN36925           101 PTDEQVKSFRQEIINKMPVCEHVKAAIAAMPQHTHPMSSLIAGVNVLAAEHihngq---kesqDEVAKNIVAKIATIAAM 177 Francisel...
YP_001359688        98 PSKEALQDFDLELRHRAYIHENLRLLFDAFPNNAHPMATLSATITALASFHkehltldndadyHTMASRLLAKMPTIAAF 177 Sulfurovu...
ABF58102            96 PNAEQLQEFILHISQEYVLPQMVRDVITSFPQNSHPMAILIASFSSLAAYYcdqk-----tdgELECKLAVAKVASIVAL 170 heartwate...
REF_mpdneb:Wbm0735  96 PNLEQHKKFFLKIQELSKVPEQVVNVIKAFPKTAHPMSILIACFATLSALYherygnnvsnenLDFGISAIAQVPAIIAM 175 Wolbachia...
AAL01655            96 PSETEHEEFARILGAEYDVPEQVMNVIRSFPRDSHPMAILIASFSALAANYhasr-----idpLTGAIIAIAKVPGIVAS 170 agent of ...
Feature 1                                                                            #  #              
1NXG_A             176 CYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPcep--------yeVNPILERAMDRILILHADHEQNASTSTVRTAGSSGA 247 Escherich...
2H12_A             183 AYKYTQGEAFIYPRNDLNYAENFLSMMFARMsep--------ykVNPVLARAMNRILILHADHEQNASTSTVRLAGSTGA 254 Acetobact...
NP_298824          177 AHRYSIGWPIRYPRNNLGYVERFLHMMFEVPsep--------lqLNPVVTKALDLLFILHADHEQNASTSTVRLVGSTGA 248 Xylella f...
XP_001433892       205 AYRTALGLPIIYPNKKYSFIENFLYMMFSTPmnd--------fnVDKVIVKALDTIFMLHADHEQNASTSTVRIAGSSLA 276 Parameciu...
YP_996181          183 AYKYSIGQPLVYPRNDLDFVSNFMQMLFAVPcep--------fkLNPAGVRAFSAVMIVQADHEQNASTFTVRSVGSSRA 254 Vermineph...
EDN36925           178 AYRHNHGKKFLEPKMEYGYAENFLYMMFADDes---------ykPDELHIKAMDTIFMLHADHEQNASTSTVRLSGSTGN 248 Francisel...
YP_001359688       178 AYRHSLGIPFIQPDIDLSFTKNFLTMMRAYPggkmhlgpngheeIKEVEVRALDTIFTLHADHEQNASTTVVRGVASTGA 257 Sulfurovu...
ABF58102           171 IYRYITNQDFIQADVGLSYSKNFIHMMFDISsy----------kFTEIVDKALDVIFVLHADHEQNASTATVRMTGSSGP 240 heartwate...
REF_mpdneb:Wbm0735 176 VYRHTNDQEFIDTDNELNYSKSFLRMMFGNAld---------ndKSTLFAKALDKIFTLHADHEQNASTAAVRVVGSAGS 246 Wolbachia...
AAL01655           171 IYRHTANLDFIQADANLEYTHHFIRMMFGDMdd----------aHRDIMHKALDAIFIMHADHEQNASTSTVRMTGSSGA 240 agent of ...
Feature 1                              ### #  #                              ########  #    #          
1NXG_A             248 NPFACIAAGIASLWGPAHGGANEAALKMLEEIssvkhIPEFFRRAKDKnd--sfRLMGFGHRVYKNYDPRATVMRETCHE 325 Escherich...
2H12_A             255 NPFACIAAGIAALWGPAHGGANEAVLKMLARIgkkenIPAFIAQVKDKns--gvKLMGFGHRVYKNFDPRAKIMQQTCHE 332 Acetobact...
NP_298824          249 NPYASVAAGITALWGPAHGGANEAVLKMLEEIgdaknVDLVIAKAKTKdknspfRLMGFGHRVYKHFDPRAKIIREMAHT 328 Xylella f...
XP_001433892       277 NPFACIAAGITALWGPYHGGANEAVLNMLKEIgnkenIPKYLQKAKSKdn--sfRLMGFGHRVYKNYDPRAKVMQEMCYN 354 Parameciu...
YP_996181          255 NPYACVAAGIASLWGPLHGGANEGVLHTLNEIgsienIPAIIKRAKDKnd--pyRLMGFGHRVYKTYDPRAKVLQRYCYE 332 Vermineph...
EDN36925           249 SPYAAIIAGITALWGPAHGGANEAVLKMLSEIgstenIDKYIAKAKDKdd--pfRLMGFGHRVYKNTDPRATAMKKNCEE 326 Francisel...
YP_001359688       258 HPYVAISSGIAALWGRAHGGANESVIAQLEMIgsvenVEKYIARAKDPdd--dfRLMGFGHRVYKNFDPRAKILKTLQDE 335 Sulfurovu...
ABF58102           241 NLFACLAAGAATLWGPAHGGANEAVINMLMNIgkpenVKQFIQKVKDSnk--stKLMGFGHRVYKNYDPRARIMCTICKE 318 heartwate...
REF_mpdneb:Wbm0735 247 NLFACLSAGVATLWGPVHGGANESVINMLKEIeqsgnVDKFIEKAKDDkd--pfKLMGFGHRVYKNYDPRARILKDACDE 324 Wolbachia...
AAL01655           241 GLFACLCAGVATLWGPAHGGANEAVIKMLAEIgspenVSSFIDKVKNNkg--ksRLMGFGHRVYKSYDPRARVLRTICKD 318 agent of ...
Feature 1                                             # #  # #                    #   #                
1NXG_A             326 VLkelg-tkdDLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPssMFTVIAAMARTVGWIAHWSEMHSDg- 403 Escherich...
2H12_A             333 VLtelgikddPLLDLAVELEKIALSDDYFVQRKLYPNVDFYSGIILKAMGIPtsMFTVLFAVARTTGWVSQWKEMIEEpg 412 Acetobact...
NP_298824          329 VLgelg-vndPLLEVALKLEEAALKDDYFVQRKLYPNVDFYSGIIYKAINIPleMFTVMFAIARTAGWVSHWLEQQVDpe 407 Xylella f...
XP_001433892       355 VLektk-ranDILELAIQLEQTALKDDYFIQRKLYPNVDFYTGIVYEALSIPtsMFTVMFAVQRSIGWICQWMEMMSEkv 433 Parameciu...
YP_996181          333 VLeaygkkddPLFKMALELERIALNDEFFIERKLYPNVDFYSGLILKTIGIPlsMFTAQFAVARTAGWISHWNEMITDpa 412 Vermineph...
EDN36925           327 ILaklghsdnPLLTVAKKLEEIALQDEFFIERKLFPNVDFYSGIILKAMGIPedMFTAIFALARTSGWISQWIEMVNDpa 406 Francisel...
YP_001359688       336 LKeelq-ldsRLMEIAGKIEEIALRDEYFVSRNLYPNIDFYTGIILTALQIPknMFTPIFVIGRTVGWITQWIEFKKDpq 414 Sulfurovu...
ABF58102           319 ILxdfg-lndPLLEVAVELENIALQDDYFIARNLYPNVDFYSGIVLKTIGIPvkMFTTLFALARTSGWSAQWYEMVSSkn 397 heartwate...
REF_mpdneb:Wbm0735 325 ILnkle-qdsKLLKIAKELEKIALKDEYFVVRKLYPNVDFYSGIIMNAINIPpsMFTPIFALARTTGWVTQWYEMINDke 403 Wolbachia...
AAL01655           319 VLdslg-rdeNLLAVAEELESQALQDEYFIERKLYPNVDFYSGIVLRAMGVPvdMYTAFFALARTSGWASQWYEMIQSde 397 agent of ...
Feature 1                #  #       
1NXG_A             404 -mKIARPRQLYTG 415 Escherichia coli
2H12_A             413 -qRISRPRQLYIG 424 Acetobacter aceti
NP_298824          408 -mKISRPRQIYTG 419 Xylella fastidiosa 9a5c
XP_001433892       434 -qRISRPRQLYVG 445 Paramecium tetraurelia strain d4-2
YP_996181          413 -vKIVRPRQLYVG 424 Verminephrobacter eiseniae EF01-2
EDN36925           407 -qKIGRPRQLYTG 418 Francisella tularensis subsp. novicida GA99-3549
YP_001359688       415 -sKIARPRQLYTG 426 Sulfurovum sp. NBC37-1
ABF58102           398 -yKISRPRQLYTG 409 heartwater rickettsia
REF_mpdneb:Wbm0735 404 -tKICRPRQLYVG 415 Wolbachia endosymbiont strain TRS of Brugia malayi
AAL01655           398 gkRISRPRQLYTG 410 agent of human granulocytic ehrlichiosis

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