Conserved Protein Domain Family
citrate_synt_like_1_2

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cd06113: citrate_synt_like_1_2 
Citrate synthase (CS) catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the oxidative citric acid cycle (TCA or Krebs cycle). Peroxisomal CS is involved in the glyoxylate cycle. This group also includes CS proteins which functions as a 2-methylcitrate synthase (2MCS). 2MCS catalyzes the condensation of propionyl-CoA (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS. The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) a carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) hydrolysis of citryl-CoA to produce citrate and CoA. CSs are found in two structural types: type I (homodimeric) and type II CSs (homohexameric). Type II CSs are unique to gram-negative bacteria. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria. Type I CS is active as a homodimer, both subunits participating in the active site. Type II CS is a hexamer of identical subunits (approximated as a trimer of dimers). Some type II CSs are strongly and specifically inhibited by NADH through an allosteric mechanism. This subgroup includes both gram-positive and gram-negative bacteria.
Statistics
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PSSM-Id: 99866
View PSSM: cd06113
Aligned: 16 rows
Threshold Bit Score: 694.012
Threshold Setting Gi: 158439952
Created: 18-Oct-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #                                                                            
AAF09126     28 KGLRDINGKGVLAGLTNISAIHSFdkEGNQi-----pGVLEYRAYNIKDIVSDLrsknRFGFEEMTYLLLFGELPSAKEL 102 Lactococcus lact...
ZP_01573988  34 KGLRNSDGSGVLVGLTEIGEVHGYf-ISEGerk-edeGHLVYRGIDVYEMVRGFqkekRFGFEEVCYLLLFAKLPNRDEL 111 Clostridium cell...
BAD50495     32 RGLRNEDGTGVLVGLTKIGNVVGYe-RIPGgglkpipGKLFYRGYDLEDLAHAIlkekRFGFEEVAYLLLSGSLPDKEEL 110 Bacteroides frag...
EDP17700     32 KGLRNEDGTGVRIGLTRVADVVGYe-ETDGgik-aipGRLLYRGVDVLDLVKGKke-sHFGYEETCFLLLFGYLPKKAEL 108 Clostridium bolt...
EDO62691     34 RGLRNPDGTGVMAGLTHICNVHGYl-IADGeki-pdeGRLTYRGINVKDIVDGCtrenRFGYEEVAWLLLFGTQPTREQL 111 Clostridium lept...
ZP_02037465  38 RGLRNADGTGVLAGITQIGNVLGYy-VQDGerf-pmeGKLIYRGIDLNDLVNGFmsenRFGFEETAYLLLFGALPTREQL 115 Bacteroides capi...
ZP_01772350  57 RGLRNADGSGVVAGLTRISDVHGYn-KVDGkvv-pdqGKLTLRGYSIEDLVNNAqaenRYGYEEVAYLLITGSLPNKEEL 134 Collinsella aero...
EDP10568     30 KGLRNEDGTGVRVGLTRIADVVGYe-IRNEekc-dvaGKLYYRGIELRELINGYskaqTYGYEEICFLLLFGYLPNKAQM 107 Eubacterium doli...
ZP_02444684  42 RGLRNADGTGVLAGITQICNVHGYl-LSEGekv-pidGELTYRGYSIRDLIAGAqaggRYCFEETAWLLLLGFLPDGMQL 119 Anaerotruncus co...
ZP_02422249  30 RGLRNNDGSGVVVGLTNICCVHGYv-IDEGekr-papGQLIYRGYDICDLISGVekdgRYGFEETAYLLLFGNLPDKEHL 107 Eubacterium sira...
Feature 1                                                                                       
AAF09126    103 QEFQILLASKrtLPEFFVRETIltnPSSDVMNSMSRCLLALASYDkkasDISIENvLEQSLALIADFPLLAIYSYQAYIH 182 Lactococcus lact...
ZP_01573988 112 SEFTELLGGLraLPVGFTEDMIlkaPSCDIMNKLARSVLASYSYDdepdGIDPKNlLRQSIELIARFPTMAAYGFQAKSH 191 Clostridium cell...
BAD50495    111 ASFRELINDNmpLEQKTKMNIIe-lEGNNIMNILARSVLEMYRFDpnpdDTSRDNlMRQSIDLISKFPTIIAYAFNMLRH 189 Bacteroides frag...
EDP17700    109 EKFCNVVRHCytIPDEFVEMNLlrlPGKNLMNKLQDAVLTLYNYDddpdNIDVYQtLVKGVNLLAKLPSIACYAYQSKVH 188 Clostridium bolt...
EDO62691    112 EKFNRILSSYreLPEYFAEDMIikaPSKDIMNKLGRSVLALYSYDsnpdETSLENiMRQSISLIARMPTIMTYAYQVKRR 191 Clostridium lept...
ZP_02037465 116 TLFQRALSVHqpLPPMFTEDMIlraPSGDVMNKLARSVLSLYSYDdapeDRSLESeMGKAIALIARLPTIAAHAYAAKRH 195 Bacteroides capi...
ZP_01772350 135 DGFCERLGAFrhLSDEYVKEFPmttVSSSIMNVLQRAVLLLYAFDaepdVITPEHeIDVAISMLARLPRIAAFAHMASVA 214 Collinsella aero...
EDP10568    108 KEFCELLRSHyeLPKDFLETKFlqmPGKNIMNRIQQAVLSLYDYDdkadATDISNtLLQGIDILAKLPSILVYAYQSKLH 187 Eubacterium doli...
ZP_02444684 120 RRFFDILSDAraLPDNFTEDMImkaASPNIMNMLARSVLALYTYDpapeDTSIENvMLQSIQLIARMPSIMAAAYQVKKR 199 Anaerotruncus co...
ZP_02422249 108 EDFKKIVTHYrtLPPYFAEDVLmrcPSSNIMNKMAQSVLALYTYDkepeNKSMESeMLKAISVIARLPAIMVGAYQVMRR 187 Eubacterium sira...
Feature 1                                                        #   #                          
AAF09126    183 YFkkeSLYIHFPDPElTTAENILRMLRpdchYSDMEAKVLDIALILHMEHGGGNNSTFTTHVVTSSGTDTYATIAAALSS 262 Lactococcus lact...
ZP_01573988 192 YYggqSLYIHSPLPQlSTAENILHMIRpdnkYTQTEAEILDLALVLHAEHGGGNNSTFAARVVSSTETDTYSAIAAAVGS 271 Clostridium cell...
BAD50495    190 ATfgrSLHIRHPQENlSIAENFLYMLKr--dYTELDARTLDLLLVLQAEHGGGNNSTFTVRVTSSTGTDTYSAIAAGIGS 267 Bacteroides frag...
EDP17700    189 YYdreSLFIHYANPEySIAENFLSLLRkdqkFTSKEANLLDTILMLHADHGGGNNSTFTNVVIASTGTDIYSTVCGGIGS 268 Clostridium bolt...
EDO62691    192 HYdkkSMYFHPIKPEhSTAESILRSIRpdkkFNDEEAKLLDLLMIIHAEHGGGNNSTFTTRVLSSSGTDTYAAISAAIGS 271 Clostridium lept...
ZP_02037465 196 YFdneSLYLHRPQEGlGVAENFLYSVRhdnqFTEEEAKLLDLCLVLHAEHGGGNNSAFACRVLSSSGTDIYSAIAAAIGS 275 Bacteroides capi...
ZP_01772350 215 KLrgsEVHVPHPTPGlSTAETILQVLRggmaFTRDEAMLLDVMLMLHAEHGGGNNSTFACRVLSSSATDPYSAYAAAIGS 294 Collinsella aero...
EDP10568    188 RYhqeSLVIHLVQHNlSIAENLLYMLRkdnrYTKLEAQLLDMLLIVHADHGGGNNSTFTNVVIASTGTDIYAAMVGAIGA 267 Eubacterium doli...
ZP_02444684 200 VYdgrSMYLHNPNPEfSTAQNILRMLRsdkqFTEDEAHLLDLCLTLHSEHGGGNNSTFAVRVLTSSRTDTYSAISAGIGA 279 Anaerotruncus co...
ZP_02422249 188 YLynsSMVMHPINLDeSLAESILSTLRdnreYTKEEAKLLDLCLMLHAEHGGGNNSTFTCRCVTSTGTDAYAAYASAISA 267 Eubacterium sira...
Feature 1           ### #  #                                         ########  #    #           
AAF09126    263 LKGPKHGGANIKAAKMLENIken--isDYNNDeei-kkYLHQILNKEVFDkQGLIYGIGHAIYSVSDPRFEVFKSYVESL 339 Lactococcus lact...
ZP_01573988 272 LKGPKHGGANIKAMNMLSDIkeh--vkDWTDDdev-rnYLIKIINKEAFDkAGLIYGMGHAVYTLSDPRTILLKEKAHQL 348 Clostridium cell...
BAD50495    268 LKGPLHGGANIQVADMFHHLqen--iqDWTNVdei-dtYFTRMLNKEVYNkTGLIYGIGHAVYTISDPRAVLLKELARDL 344 Bacteroides frag...
EDP17700    269 LKGPRHGGANIKVAEMMDAVlaetggyDADDKki--rqAVEKLMAGELYDrSGLVYGFGHAVYTVSDPRAEVLRACCMEV 346 Clostridium bolt...
EDO62691    272 LKGPKHGGANHRVMQMMECVkek--vkDWKDEdev-eaFLEKIVRKEEGDhSGLIYGQGHAIYTLSDPRAVILKTHAQRL 348 Clostridium lept...
ZP_02037465 276 LKGPRHGGANKKVMEMFGYIedg--vkDWRDDdei-rsYLSRILHKEAGDgSGLIYGMGHAIYTLSDPRAVLLKKFAKSL 352 Bacteroides capi...
ZP_01772350 295 LKGPRHGGANAKVVSMHEDIrah--vsNWEDEdev-aaYLGKILDKQAFDgTGLIYGMGHAVYTLSDPRAEVCRRYARSL 371 Collinsella aero...
EDP10568    268 MKGPRHGGANMKVRAMLEAVve---kiGYCEEeevicnLINQILDKRFFDgSGLVYGMGHAIYTLSDPRSEVLQSYIEIL 344 Eubacterium doli...
ZP_02444684 280 LKGPRHGGANFKVIEQLEYLkan--vrNWDDDgei-rdYLRRVVRREAGDrSGLIYGMGHAVYTKSDPRERLLKENALRL 356 Anaerotruncus co...
ZP_02422249 268 LKGPRHGGANIKVMEMLNCIeng--vkNWENEgev-adFLTKILNKEENDhSGLIYGMGHAVYTLSDPRAVQLKKSALKL 344 Eubacterium sira...
Feature 1                                      # #  # #                    #   #                
AAF09126    340 AKEKGleeEFKLYEKVAFLAPKVisenrkiyKTICPNVDFYSGFVYKILEIPQELFTPLFAIARIVGWLAHRMEELINm- 418 Lactococcus lact...
ZP_01573988 349 AIEKGrekEFLLYDAVERIAPDVfaqakgttKNICANVDLYSGFVYDALGIPTELFTPLFAVARIVGWCAHRIEEVVSm- 427 Clostridium cell...
BAD50495    345 AHEKGrerEFAFLELLEERAIATfgkiknngKTVSSNVDFYSGFVYEMIGLPQEIYTPLFAMARIVGWCAHRNEELNFeg 424 Bacteroides frag...
EDP17700    347 AKEQKhtrELDFYMRFERIAKEVmee--ikgKALPTNVDFYSGFAYGMLRIPRDLYTPLFVCSRVVGWVAHNIENKLYd- 423 Clostridium bolt...
EDO62691    349 AKEKGmddEFALYQLVEKLAPKAflnvkgdkKVISANVDFYSGLVYKMLGLPEDLYTPMFAVSRIAGWCAHRIEEVETs- 427 Clostridium lept...
ZP_02037465 353 AAKKGmldEFELYEAVERLTPEVlsas-gqrKVVCANVDLYSGLVYKMMGIPQELYTPLFAVARVVGWCAHRIEEVYNpg 431 Bacteroides capi...
ZP_01772350 372 AAKKDlgeEFALIERIERLAPQVmrdh-gmtKPICANIDLYTGFIYKMLGVPETLFTPLFAVARMAGWSAHRMEELFCa- 449 Collinsella aero...
EDP10568    345 AKEKQrmsEYAFYKRFEACAKKVmwe--rkhIRISSNVDFYSGFVYDMLGIPQELFTPLFVLARTVGWLAHNIENKLYd- 421 Eubacterium doli...
ZP_02444684 357 ARGTEfeaEFCLLDAIERLAPDVlhekqggdKPICANIDLYSGLVYRMLGIPDELHTPLFACARTAGWCAHRLEELSTg- 435 Anaerotruncus co...
ZP_02422249 345 AKGTEveaEFRLLDTIERLTPQLfaknkgdiKTMCANVDMYSGLVYKLLGIPVEMYTALFACARMPGWCAHRMEEIENg- 423 Eubacterium sira...
Feature 1        #  #       
AAF09126    419 NKIIRPAYESIL 430 Lactococcus lactis subsp. lactis
ZP_01573988 428 QKIIRPAYKSIS 439 Clostridium cellulolyticum H10
BAD50495    425 KRIIRPAYKNVL 436 Bacteroides fragilis YCH46
EDP17700    424 GRIMRPATKYVG 435 Clostridium bolteae ATCC BAA-613
EDO62691    428 NRIIRPAYKSLV 439 Clostridium leptum DSM 753
ZP_02037465 432 NKIIRPAYKAVA 443 Bacteroides capillosus ATCC 29799
ZP_01772350 450 HRIIRPAYRPVI 461 Collinsella aerofaciens ATCC 25986
EDP10568    422 GRIVRPATKYVG 433 Eubacterium dolichum DSM 3991
ZP_02444684 436 GKIVRPAFKSIV 447 Anaerotruncus colihominis DSM 17241
ZP_02422249 424 KRIMRPAYRTLI 435 Eubacterium siraeum DSM 15702

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