Conserved Protein Domain Family
BSuCS-II_like

?
cd06110: BSuCS-II_like 
Bacillus subtilis (Bs) citrate synthase (CS)-II_like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). 2MCS catalyzes the condensation of propionyl-coenzyme A (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. The overall CS reaction is thought to proceed through three partial reactions: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. This group contains proteins similar to BsCS-II, the major CS of the gram-positive bacterium Bacillus subtilis. A mutation in the gene which encodes BsCS-II (citZ gene) has been described which resulted in a significant loss of CS activity, partial glutamate auxotrophy, and a sporulation deficiency, all of which are characteristic of strains defective in the Krebs cycle. Streptococcus mutans CS, found in this group, may participate in a pathway for the anaerobic biosynthesis of glutamate. This group also contains functionally uncharacterized CSs of various gram-negative bacteria. Some of the gram-negative species represented in this group have a second CS isozyme found in another group. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS.
Statistics
?
PSSM-Id: 99863
View PSSM: cd06110
Aligned: 18 rows
Threshold Bit Score: 564.978
Threshold Setting Gi: 159898555
Created: 18-Oct-2007
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1           #                                                                            
BAD77021       5 RGLEGVVATTSSISSIIDDt--LTYVGYDIDDLAENAs-FEEVVYLLWHRELPTKEQLEELKKQLAENaeIPNEIIEHFk 81  Geobacillus kau...
Q59939         5 NGLKDMIACDTRISAIKDNk--LSYAGYDIADLMDNKarFEEVIYLLWNLHLPTAIELKQFEEKLRKNyaISDAIEQCIl 82  Streptococcus m...
BAB42785       6 RGLEGVIAAETKISSIIESq--LTYAGYDIDDLAENAq-FEEVIFLLWNYRLPNEEELAHLKGKLNQYmtLNPRVYTHFe 82  Staphylococcus ...
ZP_01857072   15 KGLKGIIAADSSICLVNGTigkLLYRGYNIDDLAENAt-FEDVSFLLLNGELPTATQLAAYQAELKQYrsIPPELIDALk 93  Planctomyces ma...
NP_967540     13 KGLEGVVACTTKVSFIVGDs--LNFRGYTIDDLAANSt-FEEVTYLLWNDKLPNAQELAAFSTQLRNEmaLSPDFIKVLk 89  Bdellovibrio ba...
YP_846223     10 IGLRGVTVADTKISYIDGEngiLIYRGFRIEELAERSs-FMETAYLLLNGVLPSAEELREFTAEVDAArtVPEFVIDSMr 88  Syntrophobacter...
ABK77435      83 IGLRGIPVADTRISNIDGKqgrLIYRGYDISDLSSKSn-FEEVAYLLLHDGLPTKDRLSAFNSRLVEArwIPKQMQINMg 161 Cenarchaeum sym...
YP_001544802 105 RGLAGVTFDQSQITLIDGKqgrLQYRGYSIHDLVEQTt-FEETAFLLLNGQLPTTTELDQFKHQLVAAreIPATILELIq 183 Herpetosiphon a...
ZP_02147881    9 RGLKGIYFERSGVSDIDGAkgeLSYRGYSIHDLAAHSt-FEEVCYLLIHGELPDADQLAEFDTALKAArvLPAPVLDIIr 87  Phaeobacter gal...
YP_845656     10 IGLRGITVADTKISYIDGVrgiLIYRGFRIEDLAEISs-FEETAYLLLNGGLPDKEGLAAFRDALTAAgrLPDEISESFk 88  Syntrophobacter...
Feature 1                                                                                        
BAD77021      82 lypidkVHPMAALRTAVSLL-GLYDeeadvmtkeanYRKAIRLQAKIPTIVTAFARVRKGLEPVAPRKDLSFAANFLYML 160 Geobacillus kau...
Q59939        83 iqsrqhLHPMSVLRSTVSLL-GVYNlkaeersveatYDQSIQLMAKIPTIIATFARLRQGLSPIAPRKDLGFAANFLYML 161 Streptococcus m...
BAB42785      83 eyvtdhVHPMTALRTSLSYI-AHFDpdaenesdenrYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYML 161 Staphylococcus ...
ZP_01857072   94 kl-pvsAPPMALLRSITSLA-GVYDpnaeietfenrLQISIKLISQIPTIVAAIHRVRQGLEPVEPDPELCHAGNFMYMI 171 Planctomyces ma...
NP_967540     90 ai-ptnVHPMGWLRTAVSLM-AHWDsdandngaeanLRKSVRLTAKMGTLLCAFDAIRKGKEPVAPKADKSMAWNMMYML 167 Bdellovibrio ba...
YP_846223     89 ri-preADPMDVLQGVVPFL-GVADpelmvetreanLRKAVRLIARVPAVVAAWNRIRNGLEPLPPDEGLPHAANFLWQL 166 Syntrophobacter...
ABK77435     162 nw-rkdADPMDMLQAFVAALaGYYDeefan--keasYDRAINLIGKVPTIVASWHRIRNGEDLIDPDPALSHAANFLHMM 238 Cenarchaeum sym...
YP_001544802 184 ll--kdGHPTEVLRTCLSAL-ATFDaerldgsvtatKARSIRLIAQMPCLIAAHHAIRQGKTPIQPNPTLDHAANFLYML 260 Herpetosiphon a...
ZP_02147881   88 at--kdGHPMDVLRTAVSAL-AALEpnsqqvgedafVANGIRLISQVPIIIAAHDAIRNGREVVAPDMTLSHAGNWLWML 164 Phaeobacter gal...
YP_845656     89 lw-prrSDPMDIIQASVPIL-AMIDpelaaesreanLRKAIRVIARLPEIITTWHRMRNGLQPLPHDPKLGHAGNFLWKL 166 Syntrophobacter...
Feature 1                               #  #                              ### #  #               
BAD77021     161 t-GKEPddIATEAFNKALVLHADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEAVMKMLTEI----GTV 235 Geobacillus kau...
Q59939       162 n-GRLPseLEILAMNRALVLHAEHELNASTFAARVCASTLSDIYSCVTTAIGTLKGPLHGGANERVFDMLREIr---EYG 237 Streptococcus m...
BAB42785     162 r-GELPtdIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEI----GSI 236 Staphylococcus ...
ZP_01857072  172 n-GEKPseDATRAMDLILTLHAEHGLNASTFTGRVIIATLPDIYSAVTGAIGALKGKLHGGANTEVLKTLFEI----GSV 246 Planctomyces ma...
NP_967540    168 ggGAEPkaEHVKVMDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAIGALKGPLHGGANEQVILMLQKI----GTM 243 Bdellovibrio ba...
YP_846223    167 t-GNKPddQIARDLDVCLVLHADHTFNASTFACREVVSTQAHMYAGVSAGVGALSGSLHGGANARVMKMLFEVqkevKSE 245 Syntrophobacter...
ABK77435     239 t-GKVPdkEMERIFDTCLILHADHTFNASTFAARQVASTRAHMYSAASAAIGALSGELHGGANYEVMRMLLDM----GSV 313 Cenarchaeum sym...
YP_001544802 261 q-GTIPsqQAQQIVNQILILHADHSANASTFAARVVAGTRSDWYAALTAAIAAFAGPLHGGAIEQVIAMIQAI----GTP 335 Herpetosiphon a...
ZP_02147881  165 k-GEKPtpEATRLADVDFILHAEHGANASSFAARVTIGTETNLHGGIVTALSTLAGPAHGGAAEDVMKMVHEI----GTP 239 Phaeobacter gal...
YP_845656    167 t-GKKPeeEMARAFEVSLILQADHAFSASTFACREVVSTRAHLYAGVTAGVGALSGILHGGSNALVMRVMFDMidqgMTT 245 Syntrophobacter...
Feature 1                       ########  #    #                                        # #  # # 
BAD77021     236 DnvEPYIRRKLANKEKIMGFGHRVYRKGDPRAKHLKKMSEKLtklvGEPHWYEMSTKIEEIVTSE------KALPPNVDF 309 Geobacillus kau...
Q59939       238 Dv-DSYLQEKLNSKEKIMGFGHRVYQTQDPREKYLREMARELtkgtEHDIWYQLSKKVEICMKQK------KNLIPNVDF 310 Streptococcus m...
BAB42785     237 EnvDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSRQItkdaGREELFEMSVKMEKRMAEE------KGLIPNVDF 310 Staphylococcus ...
ZP_01857072  247 EnvAPYVKKVREAKGKFMGFGHAVYQVEDPRAKHLKELSRRLgeetGEPKWYEMSIEMEKLVYEE------IKRNCNVDF 320 Planctomyces ma...
NP_967540    244 EkaQQFVKDALAAKEKVMGIGHRVYKNGDPRARILRGMSDKLtkdaGIHHMYEMSTLIDDTMFNE------KGLMPNVDF 317 Bdellovibrio ba...
YP_846223    246 DevGDWVRMRMERGEKIMGMGHAVYKTFDPRSKILKEMSLRLgvrtGHEDWYRLLTRIEQEALKEfekkgkDNIKSNVDF 325 Syntrophobacter...
ABK77435     314 EavVPWIRKRMDDGQRIMGMGHAVYKTYDPRATVLKELSRRLaa-kTGQPWFAITEKVEQATADEmkkrkgVEIYPNVDL 392 Cenarchaeum sym...
YP_001544802 336 EraADYVANLQANNQPVMGFGHRVYQTEDPRARHLRKAAQALsa-qSDNNYYAILEAVVQAMRPYm----aKGIDVNVDF 410 Herpetosiphon a...
ZP_02147881  240 DnaAAYVKTKRAAKEAVTGFGHRVYRKEDPRARHMRDGVRQLgaemGAPEWYEILQAVVEAMQPYa----rHGLNVNVDF 315 Phaeobacter gal...
YP_845656    246 KdiGAWVKGKLDRREKIMGMGHAVYKTTDPRAEILKGLCENVaiktEREEWFNFLRAIETECIREfekrgkRKIKANVDF 325 Syntrophobacter...
Feature 1                           #   #                    #  #       
BAD77021     310 YSASVYHCLGIDHDLFTPIFAVSRTSGWLAHILEQYDN-----NRLIRPRAEYTG 359 Geobacillus kaustophilus HTA426
Q59939       311 YSATVYHVLGIDSSIFTLIFAMSRVSGWIAHIQEQQKN-----NKLIRPRSHYTG 360 Streptococcus mutans
BAB42785     311 YSASVYHCMEIPHDLFTPIFAVSRSAGWIAHILEQYKD-----NRIMRPRAKYIG 360 Staphylococcus aureus subsp. aureus N315
ZP_01857072  321 YSASLQHYMGIPGDLFTCIFAASRIAGWCAHILEQLDG-----NKIIRPKANYIG 370 Planctomyces maris DSM 8797
NP_967540    318 YSATVYFSMGIPTDLFTPIFAASRISGWCAHAFEQYAN-----NRIYRPRGKWAG 367 Bdellovibrio bacteriovorus HD100
YP_846223    326 YSGSVYSMMGIPVDIMTPVFAISRISGWCSHVIEEKFAeaqdkPALYRPKAEYVG 380 Syntrophobacter fumaroxidans MPOB
ABK77435     393 YSASVYYMLDIPMDLNTPIFAISRVAGWAAHIIEEKFAeaapkPALYRPKAVYVG 447 Cenarchaeum symbiosum A
YP_001544802 411 YASVIYHLLGIPYDLFVPAFIVGRTVGWLAQIQEQYAN-----NILIRPLLAYVG 460 Herpetosiphon aurantiacus ATCC 23779
ZP_02147881  316 YSGVIYQLHGIAMDLYVPIFAIGRMPGWIIQCIEQQRG-----NILIRPLTLYNG 365 Phaeobacter gallaeciensis 2.10
YP_845656    326 YSGLLCAMLGIPLDIMTPVFAMAIAVGWCSHIIEEKFGdaqgkPVLYRPESKYVG 380 Syntrophobacter fumaroxidans MPOB

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap