1NXG,2H12


Conserved Protein Domain Family
EcCS_AthCS-per_like

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cd06107: EcCS_AthCS-per_like 
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Escherichia coli (Ec) citrate synthase (CS) gltA and Arabidopsis thaliana (Ath) peroxisomal (Per) CS_like. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the citric acid cycle (TCA or Krebs cycle). The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs, including EcCS, are strongly and specifically inhibited by NADH through an allosteric mechanism. Included in this group is an NADH-insensitive type II Acetobacter acetii CS which has retained many of the residues used by EcCS for NADH binding. C. aurantiacus is a gram-negative thermophilic green gliding bacterium; its CS belonging to this group may be a type I CS. It is not inhibited by NADH or 2-oxoglutarate and is inhibited by ATP. Both gram-positive and gram-negative bacteria are found in this group. This group also contains three Arabidopsis peroxisomal CS proteins, CYS-1, -2, and -3 which participate in the glyoxylate cycle. AthCYS1, in addition to a peroxisomal targeting sequence, has a predicted secretory signal peptide; it may be targeted to both the secretory pathway and the peroxisomes and perhaps is located in the extracellular matrix. AthCSY1 is expressed only in siliques and specifically in developing seeds. AthCSY2 and 3 are active during seed germination and seedling development and are thought to participate in the beta-oxidation of fatty acids.
Statistics
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PSSM-Id: 99860
View PSSM: cd06107
Aligned: 7 rows
Threshold Bit Score: 613.674
Threshold Setting Gi: 119481929
Created: 31-Oct-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                 #                                                                      
1NXG_A        39 GVFTFDPGFTSTASCESKITFIDGdeGILLHRGFPIDQLATDSnYLEVCYILLNGEKPTQEQYDEFKTTVTRHt-mIHEQ 117 Escherichia coli
2H12_A        46 GVFTFDPGYGETAACNSKITFIDGdkGVLLHRGYPIAQLAENAsYEEVIYLLLNGELPNKAQYDTFTNTLTNHt-lLHEQ 124 Acetobacter aceti
NP_191434     91 GLKLYDPGYLNTAPVRSSICYIDGdeGILRYRGYPIEELAESStFIEVAYLLMYGNLPSQSQLADWEFTVSQHs-aVPQG 169 thale cress
BAE66072      58 GLRVYDPGYSNTAVSHSKITYIDGlkGTIQYRGYSINDIVGRKtFIDTAHLLIWGHWPSTAEAETLQQRLDQVp-vPQDF 136 Aspergillus oryzae
XP_001260993  50 GLRVHDPGLQNTTVVETRISFSDHekGLLLFRGYTLEQLWDSD-FEDMLHLLVWGSYPTALQKKELSRKLSEEmtmVPKS 128 Neosartorya fis...
XP_001389414  87 GLRLIDPGYRNTAVKMSSITYINGneGVILYRGHPLASLIGKS-YEEITHLLIWGSLPTPEQRLRFQRRIAEAmmvVPEN 165 Aspergillus nig...
YP_001637376  44 GLMSYDPAYLNTASCKSSITYIDGdkGILEYRGYPIEQLAEQSsYLEVAYLLLYGELPSKERLAWWEYRISRHl-fLHNS 122 Chloroflexus au...
Feature 1                                                                                        
1NXG_A       118 ITRLFHAFRRDSHPMAVMCGITGALAAFyhdsldv--------nnprhrEIAAFRLLSKMPTMAAMCYKYSIGQPFVYPR 189 Escherichia coli
2H12_A       125 IRNFFNGFRRDAHPMAILCGTVGALSAFypdandi--------aipanrDLAAMRLIAKIPTIAAWAYKYTQGEAFIYPR 196 Acetobacter aceti
NP_191434    170 VLDIIQSMPHDAHPMGVLVSAMSALSIFhpdanpalsg-qdiykskqvrDKQIVRILGKAPTIAAAAYLRTAGRPPVLPS 248 thale cress
BAE66072     137 VFNVIKSFPRDGSLMGMVIAGLSALQSSdmnaipahvgktiylnnpelaDQQIIRVMANMSMLTAAAYCHHIGRDFTPPR 216 Aspergillus oryzae
XP_001260993 129 VHRTIETLPRTTSPLPLMLAGLSACLAYapesipastkpdlyqsnsnvvDRAIIRTVAAYAVVFGLVNCHRRGIPFAQPS 208 Neosartorya fis...
XP_001389414 166 VKQLVATFPRNTPPMVILCAVLTGYLADqpelipahaganlynrrpemvDEQIIRTLAVTAIAGSIAHCHMKGEELRMAD 245 Aspergillus nig...
YP_001637376 123 LVELIQAFRYDAHPMGILISSVAAMSTLypeakni--------hdpavrEKQIWRIIGQIPTIAAFAYRHRIGRPFNLPD 194 Chloroflexus au...
Feature 1                                                #  #                              ### # 
1NXG_A       190 NDLSYAGNFLNMMFstpcepyeVNPILERAMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGAN 269 Escherichia coli
2H12_A       197 NDLNYAENFLSMMFarmsepykVNPVLARAMNRILILHADHEQNASTSTVRLAGSTGANPFACIAAGIAALWGPAHGGAN 276 Acetobacter aceti
NP_191434    249 ANLSYSENFLYMLDsmgnrsykPNPRLARVLDILFILHAEHEMNCSTAAARHLASSGVDVYTACAGAVGALYGPLHGGAN 328 thale cress
BAE66072     217 AGLSYIENFLLMTGhveaatglPNPRYVNAIERLWVLIADHEMTCSTAALLQTASALPDVISCMVSAISALYGPLHGGAI 296 Aspergillus oryzae
XP_001260993 209 RHKSYLENLFQMAGlvdqttgrPDPTKLSCFRRFAMLNADHGMALSVFSALVTTSSLTDPISCLITATGAAFGPLHFGAT 288 Neosartorya fis...
XP_001389414 246 PNLSYIENILWMGRyvdnnpavTREKAAEILTKAWSLYADHEMTNSTSAFLHVSSSLADPLSAMAACCMSGYGLLHGGAI 325 Aspergillus nig...
YP_001637376 195 SSLSYTANLLYMMDymnqreyeVNPVLAKALDVLFILHADHEQNCSTSVMRSVGSSHADPYNALAAAAAALYGPLHGGAN 274 Chloroflexus au...
Feature 1         #                            ########  #    #                                  
1NXG_A       270 EAALKMLEEIssvkhIPEFFRRAKDKndsfRLMGFGHRVYKNYDPRATVMRETCHEVLkelg--tkdDLLEVAMELENIA 347 Escherichia coli
2H12_A       277 EAVLKMLARIgkkenIPAFIAQVKDKnsgvKLMGFGHRVYKNFDPRAKIMQQTCHEVLtelg-ikddPLLDLAVELEKIA 355 Acetobacter aceti
NP_191434    329 EAVLKMLAEIgtaenIPDFIEGVKNRk--rKMSGFGHRVYKNYDPRAKVIKKLADEVFsiv---grdPLIEVAVALEKAA 403 thale cress
BAE66072     297 EVAYKNIESIgsisnVPAKIARVKAGk--eRLYGYGHRVYRVTDPRFVFIREILNELSeev---ekdPLLKVAFEVDRVA 371 Aspergillus oryzae
XP_001260993 289 ESANLALREIgtpekVPKFIEEVKQGk--rRLFGYGHRSYKGLDPRVAPIRSILKDLDts-----snSLIKVAERIEQVA 361 Neosartorya fis...
XP_001389414 326 DAAYRGMREIggpqnVPKLIEKVINKe--cRLSGYGHRIYKQVDPRAKYVREMLDELTrdrdiremdPVLQVAMEIDRIA 403 Aspergillus nig...
YP_001637376 275 EAVLRMLQQIghpknVPAFIERVKKGe--mRLMGFGHRVYKNYDPRAKIIRKIAHEVFaat---aanPLLDVAMELERTA 349 Chloroflexus au...
Feature 1                # #  # #                    #   #                 #  #       
1NXG_A       348 LNDPYFIEKKLYPNVDFYSGIILKAMGipssMFTVIAAMARTVGWIAHWSEMHSDg-mKIARPRQLYTG 415 Escherichia coli
2H12_A       356 LSDDYFVQRKLYPNVDFYSGIILKAMGiptsMFTVLFAVARTTGWVSQWKEMIEEpgqRISRPRQLYIG 424 Acetobacter aceti
NP_191434    404 LSDEYFVKRKLYPNVDFYSGLIYRAMGfppeFFTVLFAVPRMAGYLSHWRESLDDpdtRIMRPQQAYTG 472 thale cress
BAE66072     372 SEDEYFTSRNLRPNADLFAAFVYKALGfppeFILPLSILSRTQGFMAHWREAMGNp-pRIWRPGQIYTG 439 Aspergillus oryzae
XP_001260993 362 SADGYFRSRGLYPNADFYGNFVFTGIGfepeLIPAAMMSQRIMGVMAHWREYMCKslqESVRYRRVSDL 430 Neosartorya fischeri NRRL 181
XP_001389414 404 STHEYFVKRNLQANADLYGSFVYTALGidsqFATVLAATARVSGVMAHWKEQTERa-pDLWRPLQVYVP 471 Aspergillus niger CBS 513.88
YP_001637376 350 LEDEYFISRKLYPNVDFYSGLIYQALRfpieYFPFLFAIPRASGWLAQWIEMLEDpeqKITRPRQVYVG 418 Chloroflexus aurantiacus J...

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