Conserved Protein Domain Family
citrate_synt_like_2

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cd06102: citrate_synt_like_2 
Citrate synthase (CS) catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) to form citrate and coenzyme A (CoA), the first step in the oxidative citric acid cycle (TCA or Krebs cycle). Peroxisomal CS is involved in the glyoxylate cycle. This group also includes CS proteins which functions as a 2-methylcitrate synthase (2MCS). 2MCS catalyzes the condensation of propionyl-CoA (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS. The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate citryl-CoA, and c) the hydrolysis of citryl-CoA to produce citrate and CoA. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs are strongly and specifically inhibited by NADH through an allosteric mechanism. This subgroup includes both gram-positive and gram-negative bacteria.
Statistics
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PSSM-Id: 99856
View PSSM: cd06102
Aligned: 31 rows
Threshold Bit Score: 214.047
Threshold Setting Gi: 21224175
Created: 18-Oct-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
  next features
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           #                                                                            
NP_104859     58 RGRSPSA--IAESAIaWGEPSIATAISTVWHGHLIYRGRDAAVLSDNAt-LEETAAVLWGLPEPirfeapapdaprqpgr 134 Mesorhizobium l...
YP_001612374  66 DARAGHGp-VAAEALrWGEPVLESALTAIGPRGPVYRGKEAVALALDDvpFEAVAELLWTGELPaegapaarpgrlsvrp 144 Sorangium cellu...
YP_628678     73 DARAGHAa-VASGALrWGEPVIDSSVSRVGAEGLAYRGHSAVTLAVEGraFEDVAELLWTGTLPeeasrwpspepafpps 151 Myxococcus xant...
ZP_01548913   53 KGRKAADd-YSGARPlTGDPVLETELTLISETGPVYRGRPAVDLAQTSt-LETVATLLWASQEDpfaesaplpppdlpeg 130 Labrenzia aggre...
NP_629954     63 ARRNRRDggINPAPAaGQELTVRTRLTLIENDRFSYRGVDAVELAARHt-YEEVAEWLWTGELRpgaafsapessvavar 141 Streptomyces co...
YP_558300     58 RGREPRVa-TAESTMrWGEPVLCSAITEITTEGPVYRNRPAMELALSGvsFESVAQFLVTGVWQdrigiwpalslpddvm 136 Burkholderia xe...
YP_001626035  24 ARKRRTAs-APAALTgAPLMVLDSPFALLTEADLYYRGRLASELALRHs-FEQVASGLWSADSAslteqpikfsrpefde 101 Renibacterium s...
YP_559287     62 QIKSGIA--PASARDtAPGLTGSSAITLMTEAGSFYRGVSAIDLAEKAt-LEDVARLLWDAGDFdpfecpatalppqsda 138 Burkholderia xe...
ZP_01463917  100 DARAGHAa-VASGALrWGEPVIDSSVTRIDARGVAYRGHLAVDLVAQGcsFEAVAELLWTGQLPasqvrwpspplpfpas 178 Stigmatella aur...
YP_645285     64 EHRRDPAr-AAEGALdWGMPVLESGISLIADGRLYYRGRDAVALSGERs-LEQVAQLIWTGSMApgdraavpglfraaar 141 Rubrobacter xyl...
Feature 1                                                                                        
NP_104859    135 asafa--------------------------qlallagqarpslgrsaaslcADAARAIgvlagal------gaeagpDP 182 Mesorhizobium l...
YP_001612374 145 aklaallpegappfvalalvvpalgahdatrhvgapsgdgriptgdaeraraRALVRSLaalvglgrdarratdaleaDG 224 Sorangium cellu...
YP_628678    152 elakllprgtppv----------aalsalvpllgardsarfasppeqeraraRRLLRHLsawvcvanapgrvthalnePT 221 Myxococcus xant...
ZP_01548913  131 lapldrlmv-------------------alaawplqdkaaytqapkllqvkgAALLRYAvaalln--------adpvdVP 183 Labrenzia aggre...
NP_629954    142 ravdalpehag--------------padrlrvaaiaaavtdplrfdlseeavLGTARVLiptlvaalp-pvlrdghdrDP 206 Streptomyces co...
YP_558300    137 llvdayaghlaaadv-------galfgmlafalgmqgrgatevadggtmrgaRQLIQAMagcmgflgpqrrfvtpnedES 209 Burkholderia xe...
YP_001626035 102 qkaaapsalgsdargtd--fmlqalliasshdpfkrdlspqsvaklgmhaiaYMIEALPskfanl-------apigstAS 172 Renibacterium s...
YP_559287    139 llkacgnfsald------------raimllpaieaanprahdlgkhgflrsgADVLRWFaawmlg-------qssparLP 199 Burkholderia xe...
ZP_01463917  179 algallpsetppl----------rvlaavvpllgatdavrfdaspgqewlraRRLLRLMggwvgvghglhrvsaaleqDS 248 Stigmatella aur...
YP_645285    142 svagcprlplggarp------vldelglalqaaasrdpaaydlrpaavvrtgARILRLLvgaatg--------eqdagET 207 Rubrobacter xyl...
Feature 1                                       #  #                             #### #  #       
NP_104859    183 VHRRLARGWSVDd---AGADAIRRALVLLADHELNASTFAARVAASTGASPAACLLAGLATLSGPRHGGAGEAVMQLAED 259 Mesorhizobium l...
YP_001612374 225 AARRLLIALGANps-kKAERAVNRALLVSADHELNASTFAVRVAASTGADLYACVSAGLAALSGPRHGGMTDRVEALVAE 303 Sorangium cellu...
YP_628678    222 VAQSLARAWNTRv--kRAPELLNRALVLCADHELNVSTFTARVAASSSADLYACLSAALAALSGPKHGGACDRVEALLVE 299 Myxococcus xant...
ZP_01548913  184 IHTQIANAFGVSs---SAKNVIRAALILCADHELNTSAFATRCAASTRAPLHAALISGLGAFAGPRHGAASDRTTAWLSD 260 Labrenzia aggre...
NP_629954    207 IARRLWTRLSGQepdePTLQALDTALGLLVDHDLAASTLAVRVAASARAHAYAAVSAGLGVIEGPLHGAAGGLAHRMLLE 286 Streptomyces co...
YP_558300    210 IAAFLLRAAGGGqsrpDSTRIMNSALVLLADHELAPATFAARIAASTNADLFNCVATAIGSQEGSSTGTSTRNIETLLLP 289 Burkholderia xe...
YP_001626035 173 VSEVLWFKLTGRlaqaNEAALLNMVLVLLLDHDLAESTFAGRVAASARAHPYAALISALSTLDSSLRGSVSVAARELIVA 252 Renibacterium s...
YP_559287    200 LHRYIGATTKCGp---KLEDAVRRVLVLSADQALHPATYVARAAASTGATPYRCVIAGLSAATGKRLPAVRVGAFSRFLA 276 Burkholderia xe...
ZP_01463917  249 LSAGLAQAWGVAv--rSAPALLDRALVLCADHELNVSTFAARVAASSGADLYACVSAALAALSGPRHGGSCDRIEALMRD 326 Stigmatella aur...
YP_645285    208 AARALQRRWVPRd--eGAAEILDRALVLCADHELNVSSFTARCVASAGATPYAAVVAGLAALGGVRHGGGTERVEAFLAE 285 Rubrobacter xyl...
Feature 1                            ########  #    #                                 #  # #     
NP_104859    260 Aarh--gaDAAIRRWLGhdrPLPAFGHPLYPDGDPRTTALSAAIPV-------DETLQQLEKAAIAMtGARPNIDFALAA 330 Mesorhizobium l...
YP_001612374 304 Agrp-eraAAAVHERLRrgePIAGVGHRLYPEGDPRAQLLLDTARAlapqsaaLRTVLALVDALRDAgLDPPSLDLGLVA 382 Sorangium cellu...
YP_628678    300 Vgrp-eraAEVIRERLRrgeAVPGFGHQLYPDGDPRTPPLVEAARSvrpetpnVRIASAVLEAMRAAgHPPPSVDFGLVM 378 Myxococcus xant...
ZP_01548913  261 Vgpd-tdiETMLSDRLSrgePLPGFGHTIYRGPDPRAACLLDVLYRsepahpfVQRLPGLITVAHDLfGEAPNIDLALAA 339 Labrenzia aggre...
NP_629954    287 Vlel-gsaAPVVAEELRagrRIPGLGHRLYPGEDPRARALFALLEDvpraapaIAAARDVQATAARHtPLHANVDLALAV 365 Streptomyces co...
YP_558300    290 Rvvt-tdqERRLDLVREygaSLFGFNYPLYPAGDPRARLLLELISGlgspsdeTASLLEFLELARSGlEAYPGVAIALVA 368 Burkholderia xe...
YP_001626035 253 Vmag-qspERAISAKLNngyGIPGFGHRIYQRRGPRAATLLAALRElppyreaLNAAAAIIAVVRARtESPVNIDFALAV 331 Renibacterium s...
YP_559287    277 EidnaqdsTEPIRIRMReseDIPGFGYSPFNAPDPRSAALWASLRVlla--tdRNFARFERAIDLAIdLTGQQPDFALLA 354 Burkholderia xe...
ZP_01463917  327 Vgrp-araASVVHERLRrgeSIPGFGHRLYPAGDPRTPPLLEVAWKvrperaaVRVARAVAEAMREEgHPPPTVDFGLVA 405 Stigmatella aur...
YP_645285    286 Veaa-gdpREVVASRLRrgeAVPGFGHRLYPEGDPRAAALLAALAEgypgseaLGLAEGAAEAAGALvGERPNVDFALAV 364 Rubrobacter xyl...
Feature 1                       #   #                #  #       
NP_104859    331 LTRGLGLPrDAPFQLFALGRSVGWAAHMVEQIVS-GSIIRPRGRYEG 376 Mesorhizobium loti MAFF303099
YP_001612374 383 IAAALGLPaGTAAALFAVGRAAGWIAHAFEQREA-GYMLRPRARYTG 428 Sorangium cellulosum 'So ce 56'
YP_628678    379 LTGALGLPsGAGATLFAVGRAAGWVAHVLEQREQ-GHLLRPRARYVE 424 Myxococcus xanthus DK 1622
ZP_01548913  340 TQKTYGLPkDAGKIIFCAARMTGWIAHALEQYAA-PEQIRPRAAYVG 385 Labrenzia aggregata IAM 12614
NP_629954    366 LTVSSGMAsTAAETIFAVARTAGWIAHALEEYGErPLRMRPSGLYSG 412 Streptomyces coelicolor A3(2)
YP_558300    369 LTRALGLPdGAAIAIWIISRTAGWAAHVLEQRTQ-AFLLRPRAKHIE 414 Burkholderia xenovorans LB400
YP_001626035 332 LIIGAEMPaDGGQTIFAVARSAGWLAHIMDEYRQaPLRLRPIGEYTG 378 Renibacterium salmoninarum ATCC 33209
YP_559287    355 AYVNRRIGaEPDANLVRLGRIAGWVAHAWEQQNE-MPLVRWKVNYNG 400 Burkholderia xenovorans LB400
ZP_01463917  406 LAAALGLPpGAATALFAVGRAAGWVAHVWEQREQ-GHVLRPRARYVE 451 Stigmatella aurantiaca DW4/3-1
YP_645285    365 LARVLGLPpGGALALFALGRAVGWVGHAIEQYAS-GAPIRPRARYVG 410 Rubrobacter xylanophilus DSM 9941

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