4CTS,1A59,1AJ8,1AMZ,1IXE,1NXG,2C6X,2CTS,2H12,6CTS


Conserved Protein Domain Family
CS_ACL-C_CCL

?
cd06099: CS_ACL-C_CCL 
Click on image for an interactive view with Cn3D
Citrate synthase (CS), citryl-CoA lyase (CCL), the C-terminal portion of the single-subunit type ATP-citrate lyase (ACL) and the C-terminal portion of the large subunit of the two-subunit type ACL. CS catalyzes the condensation of acetyl coenzyme A (AcCoA) and oxalacetate (OAA) from citrate and coenzyme A (CoA), the first step in the oxidative citric acid cycle (TCA or Krebs cycle). Peroxisomal CS is involved in the glyoxylate cycle. Some CS proteins function as a 2-methylcitrate synthase (2MCS). 2MCS catalyzes the condensation of propionyl-CoA (PrCoA) and OAA to form 2-methylcitrate and CoA during propionate metabolism. CCL cleaves citryl-CoA (CiCoA) to AcCoA and OAA. ACLs catalyze an ATP- and a CoA- dependant cleavage of citrate to form AcCoA and OAA; they do this in a multistep reaction, the final step of which is likely to involve the cleavage of CiCoA to generate AcCoA and OAA. The overall CS reaction is thought to proceed through three partial reactions and involves both closed and open conformational forms of the enzyme: a) the carbanion or equivalent is generated from AcCoA by base abstraction of a proton, b) the nucleophilic attack of this carbanion on OAA to generate CiCoA, and c) the hydrolysis of CiCoA to produce citrate and CoA. This group contains proteins which functions exclusively as either a CS or a 2MCS, as well as those with relaxed specificity which have dual functions as both a CS and a 2MCS. There are two types of CSs: type I CS and type II CSs. Type I CSs are found in eukarya, gram-positive bacteria, archaea, and in some gram-negative bacteria and are homodimers with both subunits participating in the active site. Type II CSs are unique to gram-negative bacteria and are homohexamers of identical subunits (approximated as a trimer of dimers). Some type II CSs are strongly and specifically inhibited by NADH through an allosteric mechanism. In fungi, yeast, plants, and animals ACL is cytosolic and generates AcCoA for lipogenesis. In several groups of autotrophic prokaryotes and archaea, ACL carries out the citrate-cleavage reaction of the reductive tricarboxylic acid (rTCA) cycle. In the family Aquificaceae this latter reaction in the rTCA cycle is carried out via a two enzyme system the second enzyme of which is CCL.
Statistics
?
PSSM-Id: 99853
View PSSM: cd06099
Aligned: 19 rows
Threshold Bit Score: 229.917
Threshold Setting Gi: 13473292
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic triad [active site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                          #          
4CTS_A    208 DWSHNFTNMlgy---tdaQFTELMRLYLTIHSDHEggNVSAHTSHLVGSALSDPYLSFAAAMNGLaGPLHGLAnQEVLVW 284 pig
1AMZ_A    206 DWSHNFTNMlgy---tdpQFTELMRLYLTIHSDHEggNVSAHTSHLVGSALSDPYLSFAAAMNGLaGPLHGLAnQEVLLW 282 chicken
1IXE_A    152 SHAANFLYMangv-epspEQARLMDAALILHAEHGf-NASTFTAIAAFSTETDLYSAITAAVASLkGPRHGGAnEAVMRM 229 Thermus thermophilus
2C6X_A    145 GHVENYYYMltge-qpseAKKKALETYMILATEHGm-NASTFSARVTLSTESDLVSAVTAALGTMkGPLHGGApSAVTKM 222 Bacillus subtilis
2CTS_A    208 DWSHNFTNMlgy---tdaQFTELMRLYLTIHSDHEggNVSAHTSHLVGSALSDPYLSFAAAMNGLaGPLHGLAnQEVLVW 284 pig
6CTS_A    208 DWSHNFTNMlgy---tdaQFTELMRLYLTIHSDHEggNVSAHTSHLVGSALSDPYLSFAAAMNGLaGPLHGLAnQEVLGW 284 chicken
AAF09126  199 TTAENILRMlrpdchysdMEAKVLDIALILHMEHGggNNSTFTTHVVTSSGTDTYATIAAALSSLkGPKHGGAnIKAAKM 278 Lactococcus lactis...
NP_104859 181 DPVHRRLARgws---vddAGADAIRRALVLLADHEl-NASTFAARVAASTGASPAACLLAGLATLsGPRHGGAgEAVMQL 256 Mesorhizobium loti...
XP_503380 229 DWSYNYAAMmgkg--detGFVDLLRLYFALHGDHEggNVSAHATHLVGSALSDPYLSYSAGLQGLaGPLHGLAaQEVLRF 306 Yarrowia lipolytic...
BAD77021  151 SFAANFLYMltgk-epddIATEAFNKALVLHADHEl-NASTFTARVCVATLSDIYSGITAAIGALkGPLHGGAnEAVMKM 228 Geobacillus kausto...
Feature 1                                             #                                       
4CTS_A    285 LTQLqkevgkd----vsdeklRDYIWNTLns-grVVPGYGHAVLrKTDPRYTCQREFALKhlphd----pmfklVAQLYK 355 pig
1AMZ_A    283 LSQLqkdlgad----asdeklRDYIWNTLns-grVVPGYGHAVLrKTDPRYTCQREFALKhlpsd----pmfklVAQLYK 353 chicken
1IXE_A    230 IQEIgtp-----------eraREWVREKLak-keRIMGMGHRVYkAFDPRAGVLEKLARLvaekhgh-skeyqiLKIVEE 296 Thermus thermophilus
2C6X_A    223 LEDIgek-----------ehaEAYLKEKLek-geRLMGFGHRVYkTKDPRAEALRQKAEEvagndrdldlalhvEAEAIR 290 Bacillus subtilis
2CTS_A    285 LTQLqkevgkd----vsdeklRDYIWNTLns-grVVPGYGHAVLrKTDPRYTCQREFALKhlphd----pmfklVAQLYK 355 pig
6CTS_A    285 LAQLqkaxxxa----gadaslRDYIWNTLns-grVVPGYGHAVLrKTDPRYTCQREFALKhlpgd----pmfklVAQLYK 355 chicken
AAF09126  279 LENIkenisdynndeeikkylHQILNKEVfdkqgLIYGIGHAIYsVSDPRFEVFKSYVESlakekgl-eeefklYEKVAF 357 Lactococcus lactis...
NP_104859 257 AEDAarh------------gaDAAIRRWLgh-drPLPAFGHPLYpDGDPRTTALSAAIPVde-----------tLQQLEK 312 Mesorhizobium loti...
XP_503380 307 ILDMeknvpkg----ysnedlVKYLWSVLks-grVIPGYGHAVLrKPDPRFEALMNFANSrqeird--dpvfqlVSRTSE 379 Yarrowia lipolytic...
BAD77021  229 LTEIgtv-----------dnvEPYIRRKLan-keKIMGFGHRVYrKGDPRAKHLKKMSEKltklvge-phwyemSTKIEE 295 Geobacillus kausto...
Feature 1                         #                                                   
4CTS_A    356 IVPNVlleqg-kaknPWPNVDAHSGVLLQYYgmtemnYYTVLFGVSRALGVLAQLIWSRALgfPLERPKSMS 426 pig
1AMZ_A    354 IVPNVlleqg-kaknPWPNVDAHSGVLLQYYgmtemnYYTVLFGVSRALGVLAQLIWSRALgfPLERPKSMS 424 chicken
1IXE_A    297 EAGKVlnp-----rgIYPNVDFYSGVVYSDLgfs-leFFTPIFAVARISGWVGHILEYQELdnRLLRPGAKY 362 Thermus thermophilus
2C6X_A    291 LLEIYkpg-----rkLYTNVEFYAAAVMRAIdfd-deLFTPTFSASRMVGWCAHVLEQAENn-MIFRPSAQY 355 Bacillus subtilis
2CTS_A    356 IVPNVlleqg-kaknPWPNVDAHSGVLLQYYgmtemnYYTVLFGVSRALGVLAQLIWSRALgfPLERPKSMS 426 pig
6CTS_A    356 IVPNVlleqg-aaanPWPNVDAHSGVLLQYYgmtemnYYTVLFGVSRALGVLAQLIWSRALgfPLERPKSMS 426 chicken
AAF09126  358 LAPKVisenrkiyktICPNVDFYSGFVYKILeip-qeLFTPLFAIARIVGWLAHRMEELINmnKIIRPAYES 428 Lactococcus lactis subsp. ...
NP_104859 313 AAIAMt--------gARPNIDFALAALTRGLglp-rdAPFQLFALGRSVGWAAHMVEQIVSg-SIIRPRGRY 374 Mesorhizobium loti MAFF303099
XP_503380 380 VATGVltehg-ktknPHPNVDSSSGVLFHHYgihetlYYTVTFGVSRALGPLPQLVWDRILglPIERPKSLS 450 Yarrowia lipolytica CLIB122
BAD77021  296 IVTSEk--------aLPPNVDFYSASVYHCLgid-hdLFTPIFAVSRTSGWLAHILEQYDNn-RLIRPRAEY 357 Geobacillus kaustophilus H...

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap