1PPL,3APR


Conserved Protein Domain Family
Aspergillopepsin_like

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cd06097: Aspergillopepsin_like 
Click on image for an interactive view with Cn3D
Aspergillopepsin_like, aspartic proteases of fungal origin.
The members of this family are aspartic proteases of fungal origin, including aspergillopepsin, rhizopuspepsin, endothiapepsin, and rodosporapepsin. The various fungal species in this family may be the most economically important genus of fungi. They may serve as virulence factors or as industrial aids. For example, Aspergillopepsin from A. fumigatus is involved in invasive aspergillosis owing to its elastolytic activity and Aspergillopepsins from the mold A. saitoi are used in fermentation industry. Aspartic proteinases are a group of proteolytic enzymes in which the scissile peptide bond is attacked by a nucleophilic water molecule activated by two aspartic residues in a DT(S)G motif at the active site. They have a similar fold composed of two beta-barrel domains. Between the N-terminal and C-terminal domains, each of which contributes one catalytic aspartic residue, there is an extended active-site cleft capable of interacting with multiple residues of a substrate. Although members of the aspartic protease family of enzymes have very similar three-dimensional structures and catalytic mechanisms, each has unique substrate specificity. The members of this family has an optimal acidic pH (5.5) and cleaves protein substrates with similar specificity to that of porcine pepsin A, preferring hydrophobic residues at P1 and P1' in the cleave site. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133161
View PSSM: cd06097
Aligned: 23 rows
Threshold Bit Score: 249.91
Threshold Setting Gi: 34500105
Created: 18-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic motif [active site]
Evidence:
  • Comment:Catalytic motif in both N- and C-terminal lobes of this family.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          ####                                                          
1PPL_E        17 YITPVTIGg--tTLNLNFDTGSADLWVFstelpasqqsghs------vynpsatgkelsgYTWSISYGDGSSASGNVf-t 87  Penicillium jan...
XP_370150     93 YLSPIMVGepaqMVWMNLDTGSSDTWMYasttgeghkgdqkf----yapevsktsthvpnTSWWIEYGDGTYASGDVw-k 167 Magnaporthe gri...
EAT76745      89 FYVPTVIGn--qTFKMLYDTGSADLWVIsedgpqwqadeghp-----lyhpsssaellpnHNWSIKYAYGQSASGVVy-l 160 Phaeosphaeria n...
CAC28744     106 YLCEIGIGtpqqKVKLDFDTGSADLWVRctdssllhhadkkf----dpkksdtfqesktdQTWKIQYGDGSTASGTVg-t 180 Neurospora crassa
XP_001270205  90 YLARVSIGtpeqRFNVAVDSAVGGLWVWstnlsaetiaghragtgaifdgeksstfsggeGVWRVEYGDGASAGGIVg-r 168 Aspergillus cla...
XP_390994     87 FIADIKVGtppqTLKVAIDTGSADLPNAsktah-----------------------rvrgAEWEVEYIDGTHADGIVy-l 142 Gibberella zeae...
BAE54715      89 FFMPVSIGtpaqTFNLVLDTASVDFWVRsselpadtlpkgeeeshtfdpeksstfkniedSPWKVTYVDGSSVSGSIg-t 167 Aspergillus oryzae
XP_001909842  93 YLSRVGFGtpfqYLNVDLDTGSADVWVYssetktktrredif-----eleksstaelvngSEWRITYGDSSYAWGHVy-h 166 Podospora anser...
XP_365499     91 WLNQVQVGtppqNLTVQLDTGSAEFWVFadthptakqagrgv----yspsnsstaalipgITWSKGYVGGDSASGDAvyn 166 Magnaporthe gri...
XP_368263    106 YLCEVKIGtpaqKLELQLDTASSDIWVFssgssrgvnrtnf------dawksssfscqsdKTWVMKRPDGSSISGEVg-t 178 Magnaporthe gri...
Feature 1                                                                                        
1PPL_E        88 DSVTVGgvt---ahgQAVQAaqqisaqfqqdtnNDGLLGLAFSSintvqp----qsqttFFDTVKSsla----qpLFAVA 156 Penicillium jan...
XP_370150    168 DTLSIGdve---iknMTIQTalmasvamvtdvnMSGLAGLCPNHpstvmps--qptlleKLEPVLDe-------fVFAAD 235 Magnaporthe gri...
EAT76745     161 DTVKAGplt---atkQAIEAatqis----fetaTDGILGLAPGIinqvqp----erqktFFETLAPtlq----rnLFAAN 225 Phaeosphaeria n...
CAC28744     181 DVITVGglq---iknQAIELakkvss-afssgeADGLLGLAFSTintiesdgkpdpqptPVENMISqedipkeaeLFTSA 256 Neurospora crassa
XP_001270205 169 EKIVLGemt---iqkQEFGLadvlsa-qfargtADGVLGLGFGSlgpdgpr-glveamcRTGDVSSsv------eLFTVR 237 Aspergillus cla...
XP_390994    143 DTVRLGsfelkntpiQSAQIiaarf---esetgVTGIMGLAKTLpsnvepp--tpslldKLRPLLDk-------aVFTVD 210 Gibberella zeae...
BAE54715     168 EDITIGgva---fktQPVQLaktmsp-mftqfsADGVLGLAFGHinhpqq---dikalaERLNTRDdreq--sakLFTAK 238 Aspergillus oryzae
XP_001909842 167 DSIDIGgip---lhnAVVQSavdvsqslssdkdIDGIFGLAYDLhsqvr-----pkqptVLSTLKShld----kpVFTAD 234 Podospora anser...
XP_365499    167 DTVRVGdlv---sqsQTILPataasa-syvnssYDGILGLDLADytatlpi-efkgppnFFEAIKPslp----apLFGVD 237 Magnaporthe gri...
XP_368263    179 DTVVVGgle---vpdQLLGLgkvvggtstermvADGTLGLSMPQtryiqrngvndpqtsFVARMAEakilpedaqLFTTA 255 Magnaporthe gri...
Feature 1                                                                                    ####
1PPL_E       157 Lkh------qqPGVYDFGFIDssk---ytgSLTYTGVdns------qgFWSFNVdsytags-----qsgdgfSGIADTGT 216 Penicillium jan...
XP_370150    236 Lry------qdTGRFRFGHVPksd---yegEIHWARMnkt------skFWQFDInsvhvggt--nillqstwSFIADTGT 298 Magnaporthe gri...
EAT76745     226 Hrv------dgKGTWDFGYIDesk---ytgDITWAPVsgt------qkHWQVDVgeyavgqg--sfssepvgEVIVDSGS 288 Phaeosphaeria n...
CAC28744     257 FysarddkseeKSFYTFGWVDedlvkasgkDITWTPIdns------egFWKFPSesatvdgd---nvsvsgnTAIADTGT 327 Neurospora crassa
XP_001270205 238 Lgssg--aeaeESFCTFGFVDeetvkifggEVYYTPIdds------qgFWMFDSgsavvngk---tvaregnKAIVDSGT 306 Aspergillus cla...
XP_390994    211 Lrr------naTGRFDFGYVDesr---akdKISWVSTrnd------shHWDVTFdmtawtgar-rvwmsrsfEATIDTGT 274 Gibberella zeae...
BAE54715     239 Lgswgd-sdlkKSFCTFGSIDedavrycghDVHHTPIdns------rgYWMFESpsatvggk---pmsrsenKAVVDTDA 308 Aspergillus oryzae
XP_001909842 235 Lryq-----sdEGAYTFGYIDhhr---higEINYTPLlpn------stFWEFNFtglhvvgh--nywyisqwRVIADTGT 298 Podospora anser...
XP_365499    238 Lkq------dkESFFDFGVIPpnr---yrgDVGWTAInrfrvkdmdftRWNMTVsaysignvqtaplktrnmTGVADTGT 308 Magnaporthe gri...
XP_368263    256 Lcrptq-ggkqESFCTFGYVDedm---vdgEISWAKVdsg------kgMWMFPSasttindd---kisrsgnMAVVDSST 322 Magnaporthe gri...
Feature 1                                                                                        
1PPL_E       217 TLLLLDDSvvsqyysqvs-gaqqdsnagGYVFDCStn---lpDFSVSIsgytatvpgslinygps--------gdgstcl 284 Penicillium jan...
XP_370150    299 TLMLLPMDltkmyydqvp-gaeynewydSYTFPCNetk-nlpSWDFQIaglngtvpghyiaytnvt-------eklcygg 369 Magnaporthe gri...
EAT76745     289 VLIYLPEAvvkdyygqi--pgyvptqggSNTFPCNst---mpDLNFKVgsgvltvpgrevnygsyd-------kakgtcv 356 Phaeosphaeria n...
CAC28744     328 TLALVSDTvckalyakip-gskysyryqGYLIPSTitadqlpQLSVAVggeqfviqnedlllapa---------dddhwy 397 Neurospora crassa
XP_001270205 307 MLTLVDDEtcqriydaip-gayydeesqGYLFPSDtaanelpVVSLAVgerqfvvrkedlgfaea---------kpgyay 376 Aspergillus cla...
XP_390994    275 TLIFLPADlagaywndvpnmrinpdlgdAFTFPCEfad-elpDLLFKVpktehvlkipgrylrystv----dddpeycwg 349 Gibberella zeae...
BAE54715     309 ALTLLDDQtcqaiydsip-gafydsetqGFLIPSDinvdqlpVVQLAVgeksfamskeslmfaea---------kpgyiy 378 Aspergillus oryzae
XP_001909842 299 TLMLLSPDivnmyydavpnatsdrsfggLWHYPCNtt---lpDFEIGFangwvarvpgrymnytty------ddlpgrcm 369 Podospora anser...
XP_365499    309 TLMYLDKPvvqeyysqvp-gsrydstsaGWVFPCDggp--mpDFSFGVgpmesatvitvpgiylnwtvndqakrecfggl 385 Magnaporthe gri...
XP_368263    323 PLILLSDEaceafykkie-gakysrlrqGWLVPGGetq-gmpELRVAVgetefgistse--------------------y 380 Magnaporthe gri...
Feature 1                                                 
1PPL_E       285 ggiqsnsgigFSIFGDiFLKSQYVVFDSdg----pQLGFAP 321 Penicillium janthinellum
XP_370150    370 iqpwsaetygFGILGDvFLKAVYAVFDVqn----kTVGFAN 406 Magnaporthe grisea 70-15
EAT76745     357 gaitmqlnmkYSVLGSiWMRNYYVIHSNedg--alKMGFAL 395 Phaeosphaeria nodorum SN15
CAC28744     398 ggvqsrgtmpFDILGDtFLKSIYAIWDQgn----nRFGAVP 434 Neurospora crassa
XP_001270205 377 ggiqsrgsmkMDVLGGtFLMGLYAIFDVgn----lRFGAVQ 413 Aspergillus clavatus NRRL 1
XP_390994    350 gmqsadglggVTIIGDmAIKAWYVAFDLea----nKVGFAN 386 Gibberella zeae PH-1
BAE54715     379 ggvqsrgsleFDVLGGtFLDGLYAIFDFg------SLRFGA 413 Aspergillus oryzae
XP_001909842 370 gglqpfmseeFGILGDiFLKAVYAVFDIgg----gKVGFAD 406 Podospora anserina DSM 980
XP_365499    386 qeainfqgasISLFGTvAMKAAYVIFEDrrpdadpRIGWAV 426 Magnaporthe grisea 70-15
XP_368263    381 afspadgdmfFGVVQS-RGNNEFDVLGVpf----lRSVYAP 416 Magnaporthe grisea 70-15

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