Conserved Protein Domain Family
H2MP_like-1

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cd06068: H2MP_like-1 
Putative [NiFe] hydrogenase-specific C-terminal protease. Sequence comparison shows similarity to hydrogenase specific C-terminal endopeptidases, also called Hydrogen Maturation Proteases (H2MP). Maturation of [FeNi] hydrogenases includes formation of the nickel metallocenter, proteolytic processing and assembly with other subunits. Hydrogenase maturation endopeptidases are responsible for the proteolytic processing, liberating a short C-terminal peptide by cleaving after a His or an Arg residue, e.g., HycI (E. coli) is involved in processing of HypE (the large subunit of hydrogenases 3). This cleavage is nickel dependent.
Statistics
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PSSM-Id: 99878
View PSSM: cd06068
Aligned: 10 rows
Threshold Bit Score: 167.512
Threshold Setting Gi: 38637735
Created: 27-Dec-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #                                                     #             
YP_001105262   6 LVAGIGNVFLGDDGFGVEVVGRLLeeeh-------pagVRVADFGVRGVHLAYELMNG-YDAVVLVDAVPlseePGTVQV 77  Saccharopolyspo...
ZP_01119569    9 LVAGVGNELKQDDAFGIEFAKKFRdavs------hldhIMVMEVGIGGIHLVQELHAG-YDILILADAVAwgeeAGHIYF 81  Robiginitalea b...
YP_704553      8 LVAGVGNIFFGDDGFGPEVLRGVAtrtl-------ppgVRAVDFGIRGTHLAYELLDG-WGALLLVDAVPgrgePGALRA 79  Rhodococcus sp....
YP_827768      6 LVAGVGNIFLGDDAFGSEVARRMLnekw-------pedVRVVDFGIRGFDLTFALLEG-YDTVILVDATPrggaPGTLYT 77  Solibacter usit...
YP_712614     14 LVAGVGNIFLGDDGFGVEVVRRLDpanl-------pgeVDVADYGIRGLHLAFALLDGrYDTVILVDALPsdepPGTLTV 86  Frankia alni AC...
YP_953229     13 LVAGIGNIFLGDDGFGPEVIRRIPhrla-------gpcVRLKDYGIRGMHLAYDLLGP-WSALVLVDAVPdrgaPGTVHM 84  Mycobacterium v...
NP_942709      5 LVAGIGNVFLGDDGFGVEVVQRLGarqaahalppfpegVVVADFGIRGIDLCYALLDG-VDAAILVDATQrggaPGSLYV 83  Ralstonia eutro...
NP_828548     12 LVAGIGNIFLGDDGFGVETARRLAerql-------pahIEVVDIGVRGVHLAYQLLDG-YDTLILVDATArgeaPGTLYL 83  Streptomyces av...
YP_001506825   4 LVAGVGNILLGDDGFGVQVARHLRrtgp-------pprVTIADYGTSGLHLAFDLCSGgYSTVVLVDALGrgyePGTVSL 76  Frankia sp. EAN...
YP_001070402   2 LIAGIGNIFLGDDGFGPEVLRRAAplit-------dprVRAVDYGIRGMHLAYDLLEP-WDLLVLVDALPdrgsPGTLHV 73  Mycobacterium s...
Feature 1                                          #                                             
YP_001105262  78 VE-----------VDLDAVepaapg-aaapvmDAHGMTPDAVFGLFRALGGsv-----gRALVVGCRPLDCse------r 134 Saccharopolyspo...
ZP_01119569   82 REm----------EKVSDIeempvfekrtflaDMHYTNPIRALMLAKALKVlp-----nEVYILGCEAAQHddf-----a 141 Robiginitalea b...
YP_704553     80 FEvg--------pDSVPAAp-----------lDAHSMDPGAVFGSLRALGGtl-----pRTVVLGCQVANTge------g 129 Rhodococcus sp....
YP_827768     78 IE-----------PELDDGeag--------evATHGMNPMRVLAMAKHMGAew-----kRLLVVGCEPAHDavnd---gg 130 Solibacter usit...
YP_712614     87 LR-----------PEVGDAptgda----aavvDAHGMSPDVVLRLVRDLGGei-----gQVVVVGCRPAVVad------r 140 Frankia alni AC...
YP_953229     85 FEad--------pETLTTPta----------lDAHAMDPGAVFACLNALGGaa-----pRTVVVGCEVETVee------g 135 Mycobacterium v...
NP_942709     84 IElpmdaqdgdgaGDPYASpv---------lmSPHEMNPALVLRALRMMGGtc-----aDVVVIGCEPADFglgegeegr 149 Ralstonia eutro...
NP_828548     84 IEhds-----pggGEPEGApv----------lDGHRMTPDTVLALLDTLCAgtggqpprRTLIVGCEPASVee------g 142 Streptomyces av...
YP_001506825  77 VDl----------TDVRAPse----------vDAHGMRPDVVLDLVDTLGGhl-----dRVLLVGCEPASVdh------q 125 Frankia sp. EAN...
YP_001070402  74 FEad--------hDALCATgg----------lDAHAMDPAAVFASLTALGGap-----pRTVVIGCEAADVte------g 124 Mycobacterium s...
Feature 1                              
YP_001105262 135 mGLSEPVAGAVDRAAGLVRELV 156 Saccharopolyspora erythraea NRRL 2338
ZP_01119569  142 vGMSEAVEDALPKAMRKLQNWL 163 Robiginitalea biformata HTCC2501
YP_704553    130 iGLSPRVEAAVGQAVDLVFDIV 151 Rhodococcus sp. RHA1
YP_827768    131 iGLSEAVRPAVAEAVEVVRRLI 152 Solibacter usitatus Ellin6076
YP_712614    141 mELSGAVRAAVDGAVDLIGDIL 162 Frankia alni ACN14a
YP_953229    136 iGLSETVAAAVPAAVRAVEDVV 157 Mycobacterium vanbaalenii PYR-1
NP_942709    150 lGLSEPVAAAVDRAVAMVEAMV 171 Ralstonia eutropha H16
NP_828548    143 iGLSPPVSDAVPEAVRLIDELL 164 Streptomyces avermitilis MA-4680
YP_001506825 126 vGLSPPVAAAVEPAARLVRSVI 147 Frankia sp. EAN1pec
YP_001070402 125 iGLSPPVAAAVPEAVRAVGAVV 146 Mycobacterium sp. JLS

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