2I8L,2E85


Conserved Protein Domain Family
H2MP_MemB-H2evol

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cd06067: H2MP_MemB-H2evol 
Click on image for an interactive view with Cn3D
Endopeptidases belonging to membrane-bound hydrogen evolving hydrogenase group. In hydrogenase 3 from E coli, the maturation of the large subunit (HycE) requires the cleavage of a C-terminal peptide by the endopeptidase HycI, before the final formation of the [NiFe] metallocenter. HycI protease is a monomer and lacks characteristic signature motifs of serine, zinc, cysteine, or acid proteases and thus its cleavage reaction is not inhibited by conventional inhibitors of serine and metalloproteases. Such hydrogenases as those from Methanosarcina barkeri (EchCE) and Rhodospirillum rubrum (CooLH) also belong to this group of membrane-bound hydrogen evolving hydrogenase. Sequence comparison of the large subunits from related hydrogenase indicates that in contrast to EchE (358 amino acids) and CooH (361 amino acids), the large subunit HycE (569 amino acids) contains an extra carboxy-terminal stretch of 32 amino acids that is cleaved during the maturation process. In the absence of this C-terminal stretch, there is no homolog of endopeptidase HycI found in these two related hydrogenase.
Statistics
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PSSM-Id: 99877
View PSSM: cd06067
Aligned: 30 rows
Threshold Bit Score: 127.668
Threshold Setting Gi: 149194126
Created: 10-Dec-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
nickel binding
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the metal binding residues in HycI by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #                                                #                  
2I8L_A         4 VLLCVGNSMMGDDGAGPLLAEKCAaap---kgnWVVIDGGSAPENDIVAIRelrPTRLLIVDATdmg-lnPGEIRIIDp- 78  Escherichia coli
NP_907948      4 VLLCVGNELRGDDGVAIALGRLVEeqm----peWSVFFGYDTPESEFGKLRelaPDVIVVADAMsg--fkEGEIEFLDl- 76  Wolinella succi...
YP_891333     11 SLLCIGNKMRGDDAVGLYVGELVEeqm----psWRVFYGQDVPENEFGAIRefaPDIIVVVDAMsg--fkDDKIEFFNl- 83  Campylobacter f...
ZP_01871224    4 ALLVVGNPLKGDDSVAIKLGEMIEkl-----egWRVFFGNDTPEDEVFKLKkysPDLVVVADAVig---iEGAEFLSL-- 73  Caminibacter me...
3257393        4 VICCIGNELKGDDAFGILVYEMLKgki---rdeALLINCGNVPENYIGKIIdadPDLVILVDAVhfg-gkPGELVIVDpe 79  Pyrococcus hori...
NP_578346     15 VICGIGNDIRGDDSFGVYIAEKLKrvik--kanILVLNCGEVPENYTGKILnfhPDLIIFIDAVnfg-gkPGEIIITDp- 90  Pyrococcus furi...
YP_184417     15 VICGIGNDVRGDDAFGVLVAERLKelvk--tpdVLILNCGEMPESYVGKIAafkPDLVVFVDAIhfg-geIGEFIIADpl 91  Thermococcus ko...
YP_001041450  27 VIACVGSPLRSDDRLGLLIYDKIKdla---nnnISIIECEYGLENCFTEIVaenPATLLIIDAVyneslnPGTTVLTSi- 102 Staphylothermus...
YP_902453     16 CFMGVGSTDYGDDGFGIRLAEKLEqnlakranaPVVIDAGTMPERYTGRIAdegYDSLVFLDAVdfg-gePGAVLVANs- 93  Pelobacter prop...
YP_425414     16 LVVGAGNPLRGDDGAGPRLVEMLTgk-----vnAALLDVGEIPEAYTRRILetrADTILFVDAAdfg-aqPGDFALLEe- 88  Rhodospirillum ...
Feature 1                    #                                                      
2I8L_A        79 DDIAEMf-mMTTHNMPLNYLIDQLKed--igEVIFLGIQPDIVGFyy---pMTQPIKDAVETVYQRL 139 Escherichia coli
NP_907948     77 SDERTYl--YSTHNLPTPILISYLRgi--csKTIFLGISVLLENVlhfsegLSQGASDSAFVALGRI 139 Wolinella succinogenes DSM 1740
YP_891333     84 ENERDYi--YSTHNLPTPVLLSYLRti--tpKTLFLGISVLLENVlgfeegLSQNAKISAKKAVQKI 146 Campylobacter fetus subsp. f...
ZP_01871224   74 QKDANYl--YSTHNIPLYLIIEFIKef--cdNVLFLGIGVDEKNLgkitfeISNKALNSLNEAFNKL 136 Caminibacter mediatlanticus ...
3257393       80 ETLGEA---FSTHSLPLKVLTKFIRent-raKVLLLGCQPRNVEFlg---vVSEEVKAAAERATNYI 139 Pyrococcus horikoshii OT3
NP_578346     91 ENTEGAg--VSTHSLPLKFLATYLKant-naKTILIGCQPKNIGLfe---dMSEEVKAVAEVLLKFL 151 Pyrococcus furiosus DSM 3638
YP_184417     92 KTLGEA---VSTHGLPLRIVASYIKeqt-gsDIVLIGCQPGSTGLfe---ePSELIKERAERLAELI 151 Thermococcus kodakarensis KOD1
YP_001041450 103 ENIREKislATTHTIPLRMILELIMkktnikNTYILGIRAKNLDIgm---hVSPEIKKVVEDFAKIL 166 Staphylothermus marinus F1
YP_902453     94 EEMASRfpqISTHKISLGMLANWIEasg-ttKAWLIGVQPESLKMgq---gVSPTVQDTLDILEELL 156 Pelobacter propionicus DSM 2379
YP_425414     89 ETIAAGh--AGTHQLPLTLFFQYLResg-ggEIVVLGIQPQSISLgt---pMSAAVSRTLASLSDLL 149 Rhodospirillum rubrum ATCC 1...

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