Conserved Protein Domain Family
H2MP_F420-Reduc

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cd06064: H2MP_F420-Reduc 
Endopeptidases belonging to F420-reducing hydrogenases group. These hydrogenases from methanogens are encoded by the fru, frc, or frh genes. Sequence comparison indicates that fruD and frcD gene products from Methanococcus voltae are similar to HycI protease of Escherichia coli and are putatively involved in the C-terminal processing of large subunits (FruA and FrcA respectively). FrhD (F420 reducing hydrogenase delta subunit) enzyme belongs to the gene cluster of 8-hydroxy-5-deazaflavin (F420) reducing hydrogenase (FRH) from the thermophilic methanogen Methanobacterium thermoautotrophicum delta H. FrhD subunit is putatively involved in the processing of the coenzyme F420 hydrogenase-processing. It is similar to those frhD genes found in Methanomicrobia and Methanobacteria. It is different from the FrhD conserved domain found in methyl viologen-reducing hydrogenase and F420-non-reducing hydrogenase iron-sulfur subunit D.
Statistics
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PSSM-Id: 99875
View PSSM: cd06064
Aligned: 20 rows
Threshold Bit Score: 186.293
Threshold Setting Gi: 124484843
Created: 10-Dec-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
nickel binding
Feature 1:nickel binding site [ion binding site]
Evidence:
  • Comment:based on sequence similarity to HybD structure, 1CFZ_A
  • Comment:Replacement of the homologous metal binding residues in HycI (hydrogenase 3 processing endopeptidase) by site-directed mutagenesis suggest a role in catalysis.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #                                                       #           
CAA43497      10 LVLGCGNILFGDDGFGYHMIKRLNelkeqypilnsekIQLIDAGTGAFSLYFIINRWrt-plKKIVIADIIDYGl-ePGT 87  Methanococcus v...
NP_614907     30 LIVGCGNELFGDDGFGPAVIKEIErrgw-----ehpdVEILDAGAGAPQNVFSLIDEds-kvEYMVVVDAVDVGa-ePGT 102 Methanopyrus ka...
YP_503754      7 VICGCGNPLFADDGFGPAVVEELQkldl------penVKVIDAGLGGPHFLFTLLAQsdepvKHLFIIDIADFGg-kPGE 79  Methanospirillu...
YP_064744      9 MIFGCGNITMGDDGFGPAVIDELHahye-----lpegVQAIDADTGIREYLFDYLLSeedrpDRIIVLDAVDFPdrnPGE 83  Desulfotalea ps...
YP_847635     11 LIFGCGNVLLGDDGFGPAVVEYLLehft-----lpddVYAVDVGTGIRDLLIDYVLMpd-kpKRIFIVDSICRPmrkPGE 84  Syntrophobacter...
EDO70535      13 VVFGCGNILYGDDGFGPAVADYLNahsr-----vpsfACVMDVGTAIRELLFNIIISdv-kpKVIVLVDAMETGl-eVGE 85  Dehalococcoides...
EDQ28872      12 LVLGCGNPLFGDDGFGPAVIEALEnass-----lpegCVVLDAGTSVREILFDICVSpe-apDRVIVVDAHHEPgcePGR 85  Desulfatibacill...
YP_001029459   9 IIAGVGNELFGDDGFGPVVIRALAdyel------pdnVKAMDVGLGGPHLIFSMLDPev--tKKLIIVDAMDFGg-kPGD 79  Methanocorpuscu...
NP_615928      9 VVAGCGTPLYADDGFGPAVVESLKglel------penVTVVDAGLGGPHFVFTLLNPes--tKTLIIVDIADFGg-ePGE 79  Methanosarcina ...
YP_001403639   7 VIVGCGNPLFGDDGFGPAVIEEMEklil------pdnVTIQDGGAGAPHFIFNFLDPev--tKKLVVVDISDFNa-kPGS 77  Candidatus Meth...
Feature 1                            #                                                           
CAA43497      88 LKKLCvEDIPNLPky-hvDAHDMPLAGMLREInddygIEIVVIGCQQKRVtapd---illELSEEVTNSIDDAVKMVVDE 163 Methanococcus v...
NP_614907    103 LLEFGpEDLDPNCrivpvDAHGWSIESALLDLnerigIDFRILGCQVKELpipe---vqpGLSDPVRKAVPKAADRAIEL 179 Methanopyrus ka...
YP_503754     80 IAIISpDDLPPGKy---rDAHSWDLTEPIHQLkn--rVKIDIIGCQPKRVsepe---feiGLSEEVTEAIPRTVQLVLKM 151 Methanospirillu...
YP_064744     84 VFYIApDSIPSQKih-dfSLHQFPTVNMLLELeqytgMEIKILAAQIECIpke----iapGLSEPMSKAVPAACEMISQI 158 Desulfotalea ps...
YP_847635     85 LLEVDlSELGVQKqh-dfAVHQFPSTNLLEELrseggVEVRVLAVQVKALpee----vrpGISDEVAAAVPRACEWLLEQ 159 Syntrophobacter...
EDO70535      86 LGHLTvEQVSPEKin-dfSLHLMPTLNMLKELhadggVKIDVLAVKPERIpee----meqGLTEKVQKAVVETARYITDT 160 Dehalococcoides...
EDQ28872      86 IREIGvEGLAHEKls-dfSMHQFPTMNLLAEFadktgKEVRVFVAQTCGIpdr----mapGMSPAVEAAVGRMVRFLLKL 160 Desulfatibacill...
YP_001029459  80 VTKIPaDLLPEGRgerymDAHSGNLLDPIGRLkg--kMDIVIIGCQPGYIpapdsedfalELSPEVQGAVPKTVTVILQE 157 Methanocorpuscu...
NP_615928     80 LRWLAvDELPVGSy---rDAHSWDLAEPLQYIkd--dIRIRVLACQKKYVstpe---mviGITDEVQRAIPDAVSEILKE 151 Methanosarcina ...
YP_001403639  78 ISKISgDTLKPGAy---iDPHSWDGVDQLCRIsa--kIDTTVVMCQPAKAikkgdadveiGLSEDVKNAIPKAIQVILET 152 Candidatus Meth...
Feature 1         
CAA43497     164 L 164 Methanococcus voltae
NP_614907    180 A 180 Methanopyrus kandleri AV19
YP_503754    152 I 152 Methanospirillum hungatei JF-1
YP_064744    159 L 159 Desulfotalea psychrophila LSv54
YP_847635    160 V 160 Syntrophobacter fumaroxidans MPOB
EDO70535     161 Y 161 Dehalococcoides sp. VS
EDQ28872     161 I 161 Desulfatibacillum alkenivorans AK-01
YP_001029459 158 L 158 Methanocorpusculum labreanum Z
NP_615928    152 I 152 Methanosarcina acetivorans C2A
YP_001403639 153 I 153 Candidatus Methanoregula boonei 6A8

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