Conserved Protein Domain Family
Ribosomal_L12p

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cd05832: Ribosomal_L12p 
Ribosomal protein L12p. This subfamily includes archaeal L12p, the protein that is functionally equivalent to L7/L12 in bacteria and the P1 and P2 proteins in eukaryotes. L12p is homologous to P1 and P2 but is not homologous to bacterial L7/L12. It is located in the L12 stalk, with proteins L10, L11, and 23S rRNA. L12p is the only protein in the ribosome to occur as multimers, always appearing as sets of dimers. Recent data indicate that most archaeal species contain six copies of L12p (three homodimers), while eukaryotes have four copies (two heterodimers), and bacteria may have four or six copies (two or three homodimers), depending on the species. The organization of proteins within the stalk has been characterized primarily in bacteria, where L7/L12 forms either two or three homodimers and each homodimer binds to the extended C-terminal helix of L10. L7/L12 is attached to the ribosome through L10 and is the only ribosomal protein that does not directly interact with rRNA. Archaeal L12p is believed to function in a similar fashion. However, hybrid ribosomes containing the large subunit from E. coli with an archaeal stalk are able to bind archaeal and eukaryotic elongation factors but not bacterial elongation factors. In several mesophilic and thermophilic archaeal species, the binding of 23S rRNA to protein L11 and to the L10/L12p pentameric complex was found to be temperature-dependent and cooperative.
Statistics
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PSSM-Id: 100110
View PSSM: cd05832
Aligned: 16 rows
Threshold Bit Score: 71.7593
Threshold Setting Gi: 118194947
Created: 2-Aug-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
O28780         1 MEYVYAALLLHSAGKEITEDNVKAVLEAAGVEVDEARVKALVAALEGVNIDEAIqkaam--pmavAAAPAAAAAApaeea 78  Archaeoglobus f...
P96040         1 MEYIYASLLLHSAKKEISEDALKNVLTAAGISVDEVRLKAVVAALKEVNIDEVLkn--------aAAMPVAVAAQpqatq 72  Sulfolobus solf...
133082         1 MEYIYAALLLNSANKEVTEEAVKAVLVAGGIEANDARVKALVAALEGVDIAEAIakaai--apvaAAAPVAAAAAp---- 74  Methanococcus v...
P05768         1 MEYVYAALILNEADEELTEDNITGVLEAAGVDVEESRAKALVAALEDVDIEEAVee--------aAAAPAAAPAAsgsdd 72  Halobacterium s...
NP_393837      1 MEYVYGALLLHAAGKEVDEEKLKKVLEDVGVQVDEARLKTLVSGLKDVNIDEVLknas---vaqvATAPAPAAEEkkkee 77  Thermoplasma ac...
NP_559491      1 MEYIYGALLLHYAKQEINEENLARVLQAAGITPDEVRIKTLVAALKEVNIEEAIksaa---fapvAAAPAAAAPAataaa 77  Pyrobaculum aer...
ABK77865       1 MEYVYAALLLHKLKKEVSEENLTSVVKASGAEVNEAQVKSLVAALADVDIDEAV-----------KAAPVAVAAAaapea 69  Cenarchaeum sym...
NP_148432      6 KAYIHLALAIYYAGGKIDEETLKKAAEAIGMQVDEAKIKMLVASLEEVNLEEVLkqavaapvaaaAAAPAAAPAAeek-- 83  Aeropyrum perni...
YP_001541522   1 MEYVYAALLLHYAKQPITEDNVTKILQSAGIQVDEVKVKALVSAVKEVNIDEAIktaaalpvaapSAAPQAAAPQaaeak 80  Caldivirga maqu...
NP_963494      1 MEYIYAALLLHKAGKEINEENLKKVLEAAGVEVNEGMVKAVVAALEGVNIDEILekas----qvtVAAPVAAPAPeqekk 76  Nanoarchaeum eq...
O28780        79 k----eeakeeEEEEEEVKE----------EEAIEGLGALF 105 Archaeoglobus fulgidus
P96040        73 aq--paaeekkEEKKEEEKKgp------seEEIASGLASLF 105 Sulfolobus solfataricus
133082        75 ----------aEVKKEEKKEdt-------tAAAAAGLGALF 98  Methanococcus vannielii
P05768        73 eaaaddgdddeEADADEAAEaedagddddeEPSGEGLGDLF 113 Halobacterium salinarum
NP_393837     78 ------pkkkeEKKKEEDKEhe-------eEEAMSGLSALF 105 Thermoplasma acidophilum DSM 1728
NP_559491     78 as---apqakaEEKKEEEEKkgp-----seEEIAGSLASLF 110 Pyrobaculum aerophilum str. IM2
ABK77865      70 a----akeeakEEKKDDGKSe---------AQAMEGLSSLF 97  Cenarchaeum symbiosum A
NP_148432     84 --------aeeEKKEEEEEKke-------eEVDLSGLSGMF 109 Aeropyrum pernix K1
YP_001541522  81 p----aeakaeEKKAEEKKE----------EETLESLASLF 107 Caldivirga maquilingensis IC-167
NP_963494     77 -------eekkEEKKEEKKE----------EEALGGLASLF 100 Nanoarchaeum equitans Kin4-M
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