Conserved Protein Domain Family
CBM20_water_dikinase

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cd05818: CBM20_water_dikinase 
Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99892
View PSSM: cd05818
Aligned: 5 rows
Threshold Bit Score: 176.155
Threshold Setting Gi: 125579082
Created: 18-Jan-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                   #                           #              ##   #  
NP_198009   73 KVRLNVRLDHQVNFGDHVAMFGSAKEIGSWKKKSPLNWSENGWVCELELDGGQVLEYKFVIV---KNDGslSWESGDNRV 149  thale cress
CAO45258     9 KVKLSILLKHQVKFGEHVVMLGSTKELGSWKKNVPMNWTENGWVCKLELRGDESIEYKFVIV---KRDKsmTWEGANNRV 85   Vitis vinifera
EAZ20228    36 LVHLQVCLEHQVKFGEHVGIIGSTKELGSWEEQVELEWTTNGWVCQLKLPGETLVEFKFVIFlvgGKDK--IWEDGNNRV 113  Japanese rice
EDQ82900    12 KVKVRVRLDHQVQFGETHAILGSAKSIGAWQEKVPMTWTEAGWVVDLEGDSGETVEYKHIIV---THDGgvIWEEGPNRT 88   Physcomitrella p...
ABK26307    83 KVQLHFRLDHQVRFGENHAILGSSKALGAWKKKLMMSWSESGWILDLETGGGEKVEYKFLIV---SRDGkiIWEGGCNRV 159  Sitka spruce
Feature 1                     
NP_198009  150 LKVPNSGNFSVVCHW 164  thale cress
CAO45258    86 LKLPKGGSFGVVCLW 100  Vitis vinifera
EAZ20228   114 VELPKDGKFDIVCHW 128  Japanese rice
EDQ82900    89 ISLPDEGDFELVTHW 103  Physcomitrella patens subsp. patens
ABK26307   160 LELPKEGNFEIVCHW 174  Sitka spruce

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