Conserved Protein Domain Family
CBM20_DSP

?
cd05817: CBM20_DSP 
Dual-specificity phosphatase (DSP), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This CBM20 domain is located at the N-terminus of a protein tyrosine phosphatase of unknown function found in slime molds and ciliated protozoans. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
?
PSSM-Id: 99891
View PSSM: cd05817
Aligned: 6 rows
Threshold Bit Score: 147.621
Threshold Setting Gi: 145478487
Created: 22-Jan-2008
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                   #                                  #               ##
XP_001032179  20 QVAFSVRCPTYFGQAVYISGNCDQLGNWNTEKATRMNWSEGNLWWCDISLpt----dVEVEYKYYVAdyedm-tnevCWE 94  Tetrahymena the...
XP_645373     14 NVTFTIVYNTQPGEILFITGSMNQLGEWDTSKAKRMTWNIGNLWDASVGFpe----sVYIQYKYFVYnein---ktyRWE 86  Dictyostelium d...
XP_001461844   1 MLTFQIQYPLAFGEAMYMCGNFEQLGNWNPVQAKRMQWCEGDNWVLTVDIgvaaaeeQDFEYKYFFGefdr--ptniEWE 78  Paramecium tetr...
XP_001425266  18 NVYFKIHYHTQPGKAIYIVGDCNILGNWVSTKGVRLQWNENDEWTVCVKIdrs--qyVKIEYKFIVNnydyptlndtLWE 95  Paramecium tetr...
XP_001448585   1 MLSFQLHYQLQFGDAIYISGDSDYFGNWSPLQAKRMKWNQGHLWTIDIPI-------HYFQYKYFVSsfnd--pnseEWE 71  Paramecium tetr...
XP_001449111   1 MIHLKIQFKTEFGQAVYISGNSKYLGQWNPKQAIRMNWTENDIWEVEIAY-------HKIEYKYFISqyes--iqtvLWE 71  Paramecium tetr...
Feature 1           #                                 
XP_001032179  95 QISNRVFr-------SQECq-------TKEIQDVFNH 117 Tetrahymena thermophila SB210
XP_645373     87 SIENRQLiv-----nNDELl------iEDAWDVKRNL 112 Dictyostelium discoideum AX4
XP_001461844  79 MGPNRIVrt----teNTIItd-----iWNHRKILFQC 106 Paramecium tetraurelia strain d4-2
XP_001425266  96 PGENRVIt------nHMIE--------NETKSEYFNC 118 Paramecium tetraurelia strain d4-2
XP_001448585  72 FGPNRLMrttepscnHQLNlekgivdvWNHRKVMFQC 108 Paramecium tetraurelia strain d4-2
XP_001449111  72 SGPNRVTt------kHTIDv-------WNYRKVCFQC 95  Paramecium tetraurelia strain d4-2

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap