Conserved Protein Domain Family
CBM20_DPE2_repeat2

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cd05816: CBM20_DPE2_repeat2 
Disproportionating enzyme 2 (DPE2), N-terminal CBM20 (carbohydrate-binding module, family 20) domain, repeat 2. DPE2 is a transglucosidase that is essential for the cytosolic metabolism of maltose in plant leaves at night. Maltose is an intermediate on the pathway from starch to sucrose and DPE2 is thought to metabolize the maltose that is exported from the chloroplast. DPE2 has two N-terminal CBM20 domains as well as a C-terminal amylomaltase (4-alpha-glucanotransferase) catalytic domain. DPE1, the plastid version of this enzyme, has a transglucosidase domain that is similar to that of DPE2 but lacks the N-terminal CBM20 domains. Included in this group are PDE2-like proteins from Dictyostelium, Entamoeba, and Bacteroides. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99890
View PSSM: cd05816
Aligned: 16 rows
Threshold Bit Score: 110.879
Threshold Setting Gi: 162669392
Created: 14-Jan-2008
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
starch bindingstarch binding
Feature 1:starch binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                      #                               #             ##   
AAR99599      154 ILQFRICCp-YLEEGTSIYVLGSSLNLGQWKiqDGLKLVYAGDSLWQAACVMGKDDFPLKYKYCKHska---gtSVECGa 229  potato
XP_001302508  156 RVYFSANCp-YVRAHQDVYISGSVPELGNWDakKGVKLADGKFPIWSGYVDFEKTSFPFDYKYVIAnkd--gtfIWETDg 232  Trichomonas v...
XP_001310739  151 QTFFSTNCp-YVRPKQVVRIVGNVPELGNWNpkNGLVMRDGCFPIWTANKEFAKSSFPFEYKYVIEnted-gtfLWEDCe 228  Trichomonas v...
NP_811059     126 GLVIKAYAp-RINKDYCLAICGNQKALGNWDpdKAIPMSDANFPEWQIELDASKLKFPLEYKFILYhkeekkadCWENNp 204  Bacteroides t...
XP_001580493  173 QVRFRLLVl-QLPPNYRVYITGDSSVFENWK--KYVPLVPINNLYWEIAIDMRNDTRPFDYKYLILkdgd-stpKWEHRp 248  Trichomonas v...
XP_001304350  251 KTHFQVNCp-YVHSTQSVVVVGSIPELGEWNplHGLKLEDGKFPIWANSIDLKATSFPFEYKYVLSnnd--gtfIWEEQn 327  Trichomonas v...
YP_001298698  127 SITFRALCpcLHHKRQVLAISGDCPALGNWDiqKTVLMEEIQPNEWTITLNVSTLEFPLSYKFVACnadskqveEWENHd 206  Bacteroides v...
YP_001301420  131 KLVIKISAp-RVEKNQCVAITGNQECLGNWHpdKALLLSCDTFPEWHIDLDAAEIRYPLEYKFLVWdndsrqplYWESDe 209  Parabacteroid...
XP_638682     141 IIHFQVKSt-YVPPNHSIYIVGSNTQLGNWRtdRAVKLSDESYPIWKVDLEFSKDQLPFSYKYIIAdkik-svvHWEYGn 218  Dictyostelium...
XP_655091     115 VVILRCKWq-SVPSGYSIYVTGSSSEFGKWDtyKALQMKAVGMSWWELSIPIENIREAFEYKYFMKkt----evVWEDGp 189  Entamoeba his...
Feature 1         #                                
AAR99599      230 SREIs-----VDVt---TGEs----RFVVLSDG 250  potato
XP_001302508  233 NRHCp-----APVsv-sKYP-----LIDYVQEW 254  Trichomonas vaginalis G3
XP_001310739  229 NRVCp-----APVs---VNEn----LIDYVQDW 249  Trichomonas vaginalis G3
NP_811059     205 NRYLa-----DPE----LKTn----ETLVISDR 224  Bacteroides thetaiotaomicron VPI-5482
XP_001580493  249 NHKFy-----FPF----ENP-----SVLIISDW 267  Trichomonas vaginalis G3
XP_001304350  328 NRHCh-----GPSl--sQYDgt-ipMTLFVNEW 352  Trichomonas vaginalis G3
YP_001298698  207 NRMLn-----NPEl--kKGEi---yLTPETEVH 229  Bacteroides vulgatus ATCC 8482
YP_001301420  210 NRILs----lVPQk---QGEtv-viSGLYFRDS 234  Parabacteroides distasonis ATCC 8503
XP_638682     219 DRWFtasathASEysstTNEd----RDFFFNDG 247  Dictyostelium discoideum AX4
XP_655091     190 NRLF------APM----LGPfigckQAVIYQED 212  Entamoeba histolytica HM-1:IMSS

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