1ACZ,1AC0


Conserved Protein Domain Family
CBM20_glucoamylase

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cd05811: CBM20_glucoamylase 
Click on image for an interactive view with Cn3D
Glucoamylase (glucan1,4-alpha-glucosidase), C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Glucoamylases are inverting, exo-acting starch hydrolases that hydrolyze starch and related polysaccharides by releasing the nonreducing end glucose. They are mainly active on alpha-1,4-glycosidic bonds but also have some activity towards 1,6-glycosidic bonds occurring in natural oligosaccharides. The ability of glucoamylases to cleave 1-6-glycosidic binds is called "debranching activity" and is of importance in industrial applications, where complete degradation of starch to glucose is needed. Most glucoamylases are multidomain proteins containing an N-terminal catalytic domain, a C-terminal CBM20 domain, and a highly O-glycosylated linker region that connects the two. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99886
View PSSM: cd05811
Aligned: 32 rows
Threshold Bit Score: 127.387
Threshold Setting Gi: 116199623
Created: 10-Sep-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
starch-bindingstarch-binding
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:starch-binding site 1 [chemical binding site]
Evidence:
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Structure:1AC0_A; Aspergillus niger glucoamylase CBM20 domain starch binding site 1 binds beta-cyclodextrin; defined at 4A contacts.
    View structure with Cn3D
  • Citation:PMID 9195884

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                     #    # ####                                         
1ACZ_A          1 CTtp-taVAVTFDLTATTTYGENIYLVGSISQLGDWETSDGIA-LSADKYTSSDPLWYVTVTLPaGESFEYKFIRIESd- 77   Aspergillus n...
1AC0_A          1 CTtp-taVAVTFDLTATTTYGENIYLVGSISQLGDWETSDGIA-LSADKYTSSDPLWYVTVTLPaGESFEYKFIRIESd- 77   Aspergillus n...
XP_001267000  526 CLsprrtVAVRFNVLATTVIGEDIFLVGSIPALGEWDARHQALkLEANEYSSITPLWYGTVMLDaGEEFEYKFIRKRVgd 605  Neosartorya f...
XP_001273135  535 CTvp-taVSVTFDELAATAYGETILIVGSIPELGSWDATKAVA-LSATKYSASNPLWFVTIDLPaGKSFEYKYIRKQTn- 611  Aspergillus c...
ABQ23180      509 CTpp-seVTLTFNALVDTAFGQNIYLVGSIPELGSWDPANALL-MSAKSWTSGNPVWTLSISLPaGTSFEYKFIRKDDgs 586  Thermomyces l...
XP_001270422  278 CKat-gvVPVTFNVQAPTNYGENIFLVGSISQLGSWSTSSAVP-LSATQYTSSNPLWTVTVQLPaGATFEYKFIRKQKd- 354  Aspergillus c...
XP_001544534  449 CVtp-dsILVTFNEVATTTLGQRIFIIGSVPELGSWDIQSAIA-LSADQYTDDNRLWHRTIQLGaGLDFEYKYLRKESge 526  Ajellomyces c...
XP_682173     513 CQvp-slVSVTFELTASTVWGEEIRLVGSSGELGYWVSERGIT-FSTDRYSSSQPIWWATVWLPaGQTVEYKYIRKGEle 590  Aspergillus n...
Q03045        505 CQ-----VSITFNINATTYYGENLYVIGNSSDLGAWNIADAYP-LSASAYTQDRPLWSAAIPLNaGEVISYQYVRQEDc- 577  creosote fungus
XP_001585240  510 CK-----YAVQFYVNATTYYGENIYVIGNTTDLGNWDLNSALP-MNAGMYTTEYPLWYVDTKLTaGDTVSYVFVRQQDc- 582  Sclerotinia s...
Feature 1                  #                           
1ACZ_A         78 -----dSVEWESDPNREYTVPQA--CgtSTATVTDTW 107  Aspergillus niger
1AC0_A         78 -----dSVEWESDPNREYTVPQA--CgtSTATVTDTW 107  Aspergillus niger
XP_001267000  606 -----gKVIWEEGQNRAFVVPNE--CgpSNATINAVW 635  Neosartorya fischeri NRRL 181
XP_001273135  612 -----gNVKWESNPNRSYKVPAT--CntLTDVKNDTW 641  Aspergillus clavatus NRRL 1
ABQ23180      587 -----sDVVWESDPNRSYNVPKD--CgaNTATVNSWW 616  Thermomyces lanuginosus
XP_001270422  355 -----gSVIWESNPNRSYTTPNG--CssSPATKTDRW 384  Aspergillus clavatus NRRL 1
XP_001544534  527 svsnatSYELEGGINAIPETGNPlsQ--MDDASRRSL 561  Ajellomyces capsulatus NAm1
XP_682173     591 -----mSYLRYNNTSMLFTCWYTg-Nf-LGWTIRPWQ 620  Aspergillus nidulans FGSC A4
Q03045        578 -----dQPYIYETVNRTLTVPAC--Gg-AAVTTDDAW 606  creosote fungus
XP_001585240  583 -----gQAPIYESGNRTVTVPECg-Tg-GTKVVRAAW 612  Sclerotinia sclerotiorum 1980

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