Conserved Protein Domain Family
CBM20_alpha_MTH

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cd05810: CBM20_alpha_MTH 
Glucan 1,4-alpha-maltotetraohydrolase (alpha-MTH), C-terminal CBM20 (carbohydrate-binding module, family 20) domain. Alpha-MTH, also known as maltotetraose-forming exo-amylase or G4-amylase, is an exo-amylase found in bacteria that degrades starch from its non-reducing end. Most alpha-MTHs have, in addition to the C-terminal CBM20 domain, an N-terminal glycosyl hydrolase family 13 catalytic domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch.
Statistics
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PSSM-Id: 99885
View PSSM: cd05810
Aligned: 13 rows
Threshold Bit Score: 150.251
Threshold Setting Gi: 126659022
Created: 10-Sep-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
starch-bindingstarch-binding
Feature 1:starch-binding site 1 [chemical binding site]
Evidence:
  • Comment:Based on the binding of substrates to the starch binding site 1 of other family members.
  • Comment:Site 1 is small relative to site 2 and exhibits minimal structural changes upon ligand binding. It acts as an initial starch recognition site.
  • Citation:PMID 9195884
  • Citation:PMID 8672460

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                    #                               #               ## 
AAA25707    451 VSVSFRCDNGATQMGDSVYAVGNVSQLGNWSPAAALRLTDtsgYPTWKGSIALPAGQNEEWKCLIRNEanat-qvrQWQG 529  Pseudomonas stu...
YP_528407   467 VETTFSCSNGSTYWGQSVYVIGNQEELGNWNPAHAVKLDAs-aYPTWTGTIELTQNTDVEWKCLKRDEqtps-ngvVWQG 544  Saccharophagus ...
ZP_01893399 473 ATVTFQCQNGTTTMGQSVYVTGNDSALGNWSPADAVKLDPt-aYPTWTGSIDLPAQTSIEWKCLKRSEtdpg-aqqQWQS 550  Marinobacter al...
ZP_01463880 561 VAVTFTCNNGQTVAGQSVYVVGSLAALGTWAPASAIKLTPs-sYPTWTGTVTLPASTGLEWKCLKRNEtdat-qgvQWQG 638  Stigmatella aur...
YP_562807   534 VAVTFNCNNGVTYAGQDVYVVGNQANLGNWNTAAAIKLSPt-gYPSWSGTINLPMSKAIEWKCIKKSAte-----vVWQG 607  Shewanella deni...
YP_435286   484 VDVSFTCDNGQTQWGQSVYAVGDLPELGGWSPAGAVKLEPt-aYPVWSGVISVPMGTQIEWKCLKRDEnnpg-agiEWQP 561  Hahella chejuen...
ZP_01730163 576 VDVSFSCQNGHTELGTSVYVAGNIKELGNWSVKDASQKLAptdYPTWTGSLTLPAKTNLEWKCVKALEssl--kitQWQG 653  Cyanothece sp. ...
YP_525790   549 ELVQFNCDNGFTQLGQSVYVVGSSPELGSWSVENAVLLAPv-nYPLWSTTISIPINTQMEWKCVKRDEqnpa-vnvEWQA 626  Saccharophagus ...
P22963      455 VNVNFRCDNGVTQMGDSVYAVGNVSQLGNWSPASAVRLTDtssYPTWKGSIALPDGQNVEWKCLIRNEadat-lvrQWQS 533  Pelomonas sacch...
P08704      560 QSINFTCNNGYTISGQSVYIIGNIPQLGGWDLTKAVKISPt-qYPQWSASLELPSDLNVEWKCVKRNEtnpt-anvEWQS 637  Klebsiella oxytoca
ZP_01951284 562 KSVTFTCYNGYTNLGQSVYAVGSVAQLGNWMPVDAVKLAPt-qYPTWSATIRVPATQSIEWKCLKRNEtnpa-lnvVWQS 639  Vibrio cholerae...
ZP_01078148 473 AFISFSCNNASTYLGQSVYVVGDIAALGNWQISQAIKLDAa-nYPNWQASISVPANTQVQWKCVKREEqdas-agvEWQA 550  Marinomonas sp....
BAD34980    865 VWATFSCENATTTFGQSVYVVGNVPQLGNWSPADAVKLEPsayPTWTGVVRNLPPSSTVEWKCIKRQEaglpntadAWEP 944  Arthrobacter gl...
Feature 1              #                  
AAA25707    530 G-----ANNSL-TPSe--GATTVGRL 547  Pseudomonas stutzeri
YP_528407   545 G-----ANNQFnTGTt--NAYPTGSF 563  Saccharophagus degradans 2-40
ZP_01893399 551 G-----GNNQVqTGGegtSSSTSGSF 571  Marinobacter algicola DG893
ZP_01463880 639 G-----GNNALtTPAs-gTASASASF 658  Stigmatella aurantiaca DW4/3-1
YP_562807   608 G-----GNNTYtTPSs-gVGSTSASF 627  Shewanella denitrificans OS217
YP_435286   562 G-----SNTVF-NSDl--INATRGGW 579  Hahella chejuensis KCTC 2396
ZP_01730163 654 NtktksGNNEVfVGNs--NVSVTGGF 677  Cyanothece sp. CCY 0110
YP_525790   627 G-----ANNVLdTGV---TAFTQASF 644  Saccharophagus degradans 2-40
P22963      534 G-----GNNQV-QAAa--GASTSGSF 551  Pelomonas saccharophila
P08704      638 G-----ANNQF-NSNd--TQTTNGSF 655  Klebsiella oxytoca
ZP_01951284 640 G-----ANNQL-HSS---SSASQGSF 656  Vibrio cholerae 1587
ZP_01078148 551 G-----ANNQVtSGNlgqTVQSSGSF 571  Marinomonas sp. MED121
BAD34980    945 G-----GNNILsTPPsgsAGITTGAF 965  Arthrobacter globiformis

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