5OPE,5O5G


Conserved Protein Domain Family
IgI_3_Robo

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cd05725: IgI_3_Robo 
Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors; member of the I-set of Ig superfamily (IgSF) domains
The members here are composed of the third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors. Robo receptors play a role in the development of the central nervous system (CNS), and are receptors of Slit protein. Slit is a repellant secreted by the neural cells in the midline. Slit acts through Robo to prevent most neurons from crossing the midline from either side. Three mammalian Robo homologs (Robo1, Robo2, Robo3), and three mammalian Slit homologs (Slit-1,Slit-2, Slit-3), have been identified. Commissural axons, which cross the midline, express low levels of Robo; longitudinal axons, which avoid the midline, express high levels of Robo. Robo1, Robo2, and Robo3 are expressed by commissural neurons in the vertebrate spinal cord and Slit-1, Slit-2, and Slit-3 are expressed at the ventral midline. Robo-3 is a divergent member of the Robo family which instead of being a positive regulator of Slit responsiveness, antagonizes Slit responsiveness in precrossing axons. The Slit-Robo interaction is mediated by the second leucine-rich repeat (LRR) domain of Slit and the two N-terminal Ig domains of Robo, Ig1 and Ig2. The primary Robo binding site for Slit2 has been shown by surface plasmon resonance experiments and mutational analysis to be the Ig1 domain, while the Ig2 domain has been proposed to harbor a weak secondary binding site. This group belongs to the I-set of IgSF domains, having A-B-E-D strands in one beta-sheet and A'-G-F-C-C' in the other. Like the V-set Ig domains, members of the I-set have a discontinuous A strand, but lack a C" strand.
Statistics
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PSSM-Id: 409390
View PSSM: cd05725
Aligned: 12 rows
Threshold Bit Score: 146.386
Threshold Setting Gi: 115934044
Created: 18-Jul-2007
Updated: 17-Aug-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Ig strand A [structural motif]
Evidence:
  • None

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         ####                                                                            
5OPE_A        203 VKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKd--DGELPKSRYEIRD-DHTLKIRKVTAGDMGSYTCVAENMVGKAEA 279  human
5O5G_A        203 VKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKd--DGELPKSRYEIRD-DHTLKIRKVTAGDMGSYTCVAENMVGKAEA 279  human
AAC38849      258 MKEPKDQVMLYGQTATFHCSVGGDPPPKVLWKKe--EGNIPVSRARILHdEKSLEISNITPTDEGTYVCEAHNNVGQISA 335  fruit fly
AAK94293      220 VKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKd--DGELPKARYEIRD-DHTLKIRKVMAGDMGSYTCVAENMVGKAEA 296  chicken
O89026        226 VKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKd--DGELPKSRYEIRD-DHTLKIRKVTAGDMGSYTCVAENMVGKAEA 302  house mouse
AAV36901      163 IRGPQNQTAVVGSSVVFQCRIGGDPLPGVLWRRtasGGNMPLRRVHVLE-DRSLKLDDVTLEDMGEYTCEADNAVGGITA 241  fruit fly
XP_967065     216 NKEPQDVVALLGKRVVFQCAVDGEPVPNVLWRRe--DGKMPIGRARILD-DKSLLIDNVQTSDEGIYICAAENVVGSITA 292  red flour beetle
XP_396192     108 LSTPANQTILADQTAEFACRVGGDPPPEILWRRn--DGKMPIGRAHILD-DKSLRIERVTSQDQGTYICDAENGVGAISA 184  honey bee
XP_001194423  216 INPPQDTITAPGEAAVLRCEVEGDPPPTVSWSRe--IGELPDGRTQQLP-DNSLRIRHTDELDDGVYICTAENIWGTSDA 292  purple urchin
CAM60060      227 LRKPSSQSVLVDQSVEFRCEAQGDPVPTIRWRKe--DGELPKGRYEIWE-DHTLKLKRLTSGDAGTYTCQAENMMGKTEA 303  zebrafish
CAM60061      260 VRRPVNQVVLADDTVDFQCEVQGDPAPTIRWKKe--EGELPRGRFEIRA-DNSLRLTQVKAEDEGSYTCLSENSVGKAEA 336  zebrafish
NP_001084250  228 LRRPSNMAVTVDDSVEFKCEARGDPVPTVRWRKd--DGDLPKSRYEIRD-DHTLKIRKVTAGDMGSYTCVAENMVGKAEA 304  African clawe...
Feature 1               
5OPE_A        280 SATLTV 285  human
5O5G_A        280 SATLTV 285  human
AAC38849      336 RASLIV 341  fruit fly
AAK94293      297 SATLTV 302  chicken
O89026        303 SATLTV 308  house mouse
AAV36901      242 TGILTV 247  fruit fly
XP_967065     293 KASLVV 298  red flour beetle
XP_396192     185 SATLTV 190  honey bee
XP_001194423  293 KARLRV 298  purple urchin
CAM60060      304 SATLTV 309  zebrafish
CAM60061      337 SGSLQV 342  zebrafish
NP_001084250  305 SAVLTV 310  African clawed frog

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