2V9Q,2VRA


Conserved Protein Domain Family
IgI_2_Robo

?
cd05724: IgI_2_Robo 
Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors; member of the I-set of Ig superfamily (IgSF) domains
The members here are composed of the second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors. Robo receptors play a role in the development of the central nervous system (CNS), and are receptors of the Slit protein. Slit is a repellant secreted by the neural cells in the midline. Slit acts through Robo to prevent most neurons from crossing the midline from either side. Three mammalian Robo homologs (Robo1, Robo2, and Robo3), and three mammalian Slit homologs (Slit-1,Slit-2, Slit-3), have been identified. Commissural axons, which cross the midline, express low levels of Robo; longitudinal axons, which avoid the midline, express high levels of Robo. Robo1, Robo2, and Robo3 are expressed by commissural neurons in the vertebrate spinal cord and Slit-1, Slit-2, Slit-3 are expressed at the ventral midline. Robo-3 is a divergent member of the Robo family which instead of being a positive regulator of Slit responsiveness, antagonizes Slit responsiveness in precrossing axons. The Slit-Robo interaction is mediated by the second leucine-rich repeat (LRR) domain of Slit and the two N-terminal Ig domains of Robo, Ig1 and Ig2. The primary Robo binding site for Slit-2 has been shown by surface plasmon resonance experiments and mutational analysis to be the Ig1 domain, while the Ig2 domain has been proposed to harbor a weak secondary binding site. This group belongs to the I-set of IgSF domains, having A-B-E-D strands in one beta-sheet and A'-G-F-C-C' in the other. Like the V-set Ig domains, members of the I-set have a discontinuous A strand, but lack a C" strand.
Statistics
?
PSSM-Id: 409389
View PSSM: cd05724
Aligned: 8 rows
Threshold Bit Score: 152.555
Threshold Setting Gi: 178847385
Created: 19-Jul-2007
Updated: 17-Aug-2020
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Ig strand A [structural motif]
Evidence:
  • None

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1         ####                                                                            
2V9Q_A        115 RQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLddk-------deRITIRg--GKLMITYTRKSDAGKYVCVGT 185  human
2VRA_A        112 RVEPKDTRVAKGETALLECGPPKGIPEPTLIWIKDGVPLddlkamsfgassRVRIVd-gGNLLISNVEPIDEGNYKCIAQ 190  fruit fly
NP_001024990  136 RVRPRTVQALGGEMAVLECSPPRGFPEPVVSWRKDDKELriq-------dmPRYTLhsdGNLIIDPVDRSDSGTYQCVAN 208  nematode
XP_785004     247 RQEPSDTMALLGEAVVLECSPPRGHPEPEVSWERNGQTVved-------giRVRILt-dGNLVISQPKSSDIGNYVCVAT 318  purple urchin
NP_061928     140 QIQPRDMVAVVGEQFTLECGPPWGHPEPTVSWWKDGKPLal--------qpGRHTVs-gGSLLMARAEKSDEGTYMCVAT 210  human
AAV36901       69 RLEPANTRVAQGEVALMECGAPRGSPEPQISWRKNGQTLnlv------gnkRIRIVd-gGNLAIQEARQSDDGRYQCVVK 141  fruit fly
CAM60093      176 RVQPSDVEVAIGEMATINCSPPVGHPEPNVTWRKDGILInss-------neHYTELk--GKLIIAPAQKNDSGVYSCIAS 246  zebrafish
XP_001194423  123 REEPVSDQLISGDSAVLECTPPRGYPTPEVTWLKDDQPIild-------nnRVKIVe-dGNLLLKELRKDDEGTYKCKAQ 194  purple urchin
Feature 1                        
2V9Q_A        186 NMVGERESEVAELTV 200  human
2VRA_A        191 NLVGTRESSYAKLIV 205  fruit fly
NP_001024990  209 NMVGERVSNPARLSV 223  nematode
XP_785004     319 NSEGAKRSAAGRLII 333  purple urchin
NP_061928     211 NSAGHRESRAARVSI 225  human
AAV36901      142 NVVGTRESATAFLKV 156  fruit fly
CAM60093      247 NMIGVRESRAARLSV 261  zebrafish
XP_001194423  195 NVAGERISKDAYLTV 209  purple urchin

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap