Conserved Protein Domain Family
STKc_MASTL
?
cd05610: STKc_MASTL 
Catalytic domain of the Serine/Threonine Kinase, Microtubule-associated serine/threonine-like kinase (also called greatwall kinase)
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The MASTL kinases in this group carry only a catalytic domain, which contains a long insertion relative to MAST kinases. MASTL, also called greatwall kinase (Gwl), is involved in the regulation of mitotic entry, which is controlled by the coordinated activities of protein kinases and opposing protein phosphatases (PPs). The cyclin B/CDK1 complex induces entry into M-phase while PP2A-B55 shows anti-mitotic activity. MASTL/Gwl is activated downstream of cyclin B/CDK1 and indirectly inhibits PP2A-B55 by phosphorylating the small protein alpha-endosulfine (Ensa) or the cAMP-regulated phosphoprotein 19 (Arpp19), resulting in M-phase progression. Gwl kinase may also play roles in mRNA stabilization and DNA checkpoint recovery. The human MASTL gene has also been named FLJ14813; a missense mutation in FLJ14813 is associated with autosomal dominant thrombocytopenia. The MASTL kinase subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Links
?
Statistics
?
PSSM-Id: 270761
Aligned: 5 rows
Threshold Bit Score: 413.121
Created: 4-Aug-2006
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
Download Cn3D
 
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the binding of peptide substrates and ATP analogs to the AGC kinases, PKA and PKB.
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1                #####   #             # #                               #            
Q96GX5     30 PSIEEFSIVKPISRGAFGKVYLGQKg---gKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSAnNV 106 human
E1C2I2     25 PSIEEFTIVKPISRGAFGKVYLGRKa---gRLYAVKVMKKADMINKNMVHQVQAERDALALSKSPFIVHLYYSLQSAnNV 101 chicken
Q6DBX4     18 PSIEDFVLVKPISRGAFGKVYLARKkc-nsKLYAIKVVKKAEMVDKNMTEQMRAERDALALSKSPFIVHLFYSLQTAtKV 96  zebrafish
NP_524860  52 PTIKDFVIIKPISRGAFGKVFLGYKnndskRLFAIKVMRKSEMINKNMVSQVITERNALALSRSQFCVSLFYSLQSLsYV 131 fruit fly
Q6NTJ3     27 PSIEEFGIVKPISRGAFGKVYLARRkn-nsKLFAVKVVKKADMINKNMVQQVQAERDALALSKSPFIVHLYYSLQSAnNI 105 African clawed frog

Feature 1        ## #   # #                                    # # ## #         ##  #         
Q96GX5    107 YLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISneGHIKLTDFGLSKVTLNRDI 186 human
E1C2I2    102 YLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEAALALDYLHRHGIIHRDLKPDNMLISnqGHIKLTDFGLSRVTLNREI 181 chicken
Q6DBX4     97 YLVMEYLIGGDVKSLLHIYGYFDEDMSLKYISEVALALDYLHRHSIIHRDLKPDNMLISneGHIKLTDFGLSKVKLDREL 176 zebrafish
NP_524860 132 YLVMEYMVGGDLKSLLAMFGYFDEPTARFYVAEMVMALQYLHQHGIVHRDIKPDNMLLSssGHVKLTDFGLSKIDMRRDL 211 fruit fly
Q6NTJ3    106 YLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVAMALDYLHRHGIIHRDLKPDNMLISnkGHIKLTDFGLSKVTLKREL 185 African clawed frog

Feature 1                                                                                     
Q96GX5    187 NMMDILttpsmakprqdysRTPGQVLSLISSLGFNTPiaeknqdpanilsaclsetsqlsqglvcpmsvdqkdttpyssk 266 human
E1C2I2    182 NMIDILttpsmakpkhdysRTPGQLLSLISSLGFYTPvgmkmpinpnsggasdslhevisplsmiekentplstklfktg 261 chicken
Q6DBX4    177 NLMDILttpslvkptkdyfRTPGQVLSLISSLGLNTPviegkrhsstvlgspmscgkvkqrnrslgsplmkrraeymnsp 256 zebrafish
NP_524860 212 EISDLIncsp-----nlnaRTPGQLLSLTSHLSFGSEkklndfgsvssgqnngmgsvatgtshllqainkhslimelsds 286 fruit fly
Q6NTJ3    186 CMMDILttpsmakpkrdysRTPGQVLSLISSLGFNTPaggrtqgslnqqtegmrgnastpllmkkreslvkgnklmiscp 265 African clawed frog

Feature 1                                                                                     
Q96GX5    267 llkscletvasnpgmpvkcltsnllqsrkrlatssassqshtfissvesechsspkwekdcqesdealgptmmswnavek 346 human
E1C2I2    262 ldtspltpvmpvrsltpallqsrerfgastassqscmylssmeseccssprlekdvkqtedemcstgtsnsrpplpssre 341 chicken
Q6DBX4    257 vctsralasnsvfspvllarsltprllksgkrldtmsvgsthscmlpsttdsencvspmwedeqnlhdvenipqlngrev 336 zebrafish
NP_524860 287 egdtslndaektsdskisgvspffsaeeanesithtcttnvnpqdsssscsfhtcnsadlskcsppleskdgaaagnaip 366 fruit fly
Q6NTJ3    266 easlsspsipvkcltpnllkcrtqfatsstssqsriclsslesecgsprwencsqdaeappyfnssrvkdssseqarskk 345 African clawed frog

Feature 1                                                                                     
Q96GX5    347 lcaksanaietkgfnkkdlelalspihnssalpttgrscvnlakkcfsgevsweaveldvnninmdtdtsqlgfhqsnqw 426 human
E1C2I2    342 vlnskdpkvlkkelesaispissndcgsrqklgterseitdtpvttldtkgivrkclsenkiweeklvarremtnemlet 421 chicken
Q6DBX4    337 dnrksrnvpltpvekrktlparaqdhrpvftplnnqvtnsrnikpdlsakrlqfgatdnsatplektvphqsvgnglikp 416 zebrafish
NP_524860 367 skrrvefvldaapcqgcklaeqdssnmatndgkhlpkidnaieasfefsmvrrrsvdernriskgpedsgvssrkgddys 446 fruit fly
Q6NTJ3    346 ptgssasqnlkrlefafspivdrrtgkkagfqdetgelsdtplatlnakgvirkclyenkaqekpkdfdktgqgelgkft 425 African clawed frog

Feature 1                                                                                     
Q96GX5    427 avdsggiseehlgkrslkrnfelvdsspckkiiqnkktcveykh--------nemtncytnqntgltvevqdlklsvhks 498 human
E1C2I2    422 assqqsplflkdpvqpvkeeeifekpgvkrsfelvdtspcqelnyvkk-tnaeykrgcwiselsaskstgltteiqslml 500 chicken
Q6DBX4    417 eplkelkssvkrafeevekspeqaeilfkkndaayersfq-----------------ipektsrahtgltgifsivgldd 479 zebrafish
NP_524860 447 schlnlnsestassiek--------------------------------------------------------------n 464 fruit fly
Q6NTJ3    426 sspdsppwlangsvapiqfndeektekmgvkrnydlvekspeqellqdkktntdykrgcaitdypvsqstgltmeinslf 505 African clawed frog

Feature 1                                                                                     
Q96GX5    499 qqndcankenivnsftdkqqtpeklpipmiaknlmceldedceknskrdylsssflcsdddrasknismnsdssfpgisi 578 human
E1C2I2    501 sgeiceskeimrcidrqqtekplvptvaknllcdldadhekdkeymnssllcaddekplgalsadsdlsfpetsvseshl 580 chicken
Q6DBX4    480 vksapkcqqfnertgpkqsspiavaknlfceledqgeeggkaepnsssstspgdernirrslslesdvsahemslvantp 559 zebrafish
NP_524860 465 vdnlsqskedfscsdysrsynvtngnemsginmnspfrnlskhfkrpdflrgmkrkinlvnrsdnmssmdtdgcsssngs 544 fruit fly
Q6NTJ3    506 lselrnsankyasdrksedkyisaprtlekldsgnpvaknllcelddncerdgevsstsegedrkerlnqdssstgmsvt 585 African clawed frog

Feature 1                                                                                     
Q96GX5    579 mesplesqpldsdrsikessfeesniedplivtpdcqektspkgvenpavqesnqkmlgpplevlktlaskrnavafrsf 658 human
E1C2I2    581 ekqlvdldkgvkdlsfeepkaedlltmspncqeasrngveadvvqnctmlcceqdnhqkhteetdtisspsekmtetvhl 660 chicken
Q6DBX4    560 qklsdakqevlsssfeeldeneisavtpmarptvatpkhssakqrrgecerslldhphglsdsmikspgflkpknvvafr 639 zebrafish
NP_524860 545 tntgltqeieilnigsstpkkrkarsspirgvlkvrslsddempinhllgpeanvanvvfstpvssqklprrdggllgkl 624 fruit fly
Q6NTJ3    586 enqidrdlshvdksikelsfeesqsenseeitpdnkgipfmaendervqskyepntsilpdslqnvlaspapasamtnpr 665 African clawed frog

Feature 1                                                                                    #
Q96GX5    659 nshinasnnsepsrmnmtsldamdiscaysgsypmaitptqkrrscmphqqtpnqiksgTPYRTPKSVRRGvapVDDGRI 738 human
E1C2I2    661 frknnvvfrsynspinvsnvsdpcsmasldimdlspacsgsyptaitplqktprqgdagTPYRTPKSVRRGaapVEGERI 740 chicken
Q6DBX4    640 sycssinrsctshlslasfdamemsasasfhnavtpvqkkrpslsnslyqtpqqmvvshTPYRTPKSVRRGperVEGAPI 719 zebrafish
NP_524860 625 katrfalplsienkkrehatadkmsgiqyhlklsddptmspinhgagnlpktpknvninTPFRTPKSVRRGa-rVSNERI 703 fruit fly
Q6NTJ3    666 rkpmvafrsynspinvsnvsepskismnsadkihfslectgsfpmavtpaqnkvqglieTPYRTPKSVRRGgiqVDHERI 745 African clawed frog

Feature 1     #####                          #     #  #                                       
Q96GX5    739 LGTPDYLAPELLLGRaHGPAVDWWALGVCLFEFLTGIPPFNDETPQQVFQNILKRDIPWPEGEEk-LSDNAQSAVEILLT 817 human
E1C2I2    741 LGTPDYLAPELLLTKpHGSAVDWWALGVCLFEFLTGIPPFNDETPAQVFQNILKRDIPWPEGEEk-LSDNAQNAIDILLT 819 chicken
Q6DBX4    720 LGTPDYLAPELLLGKpHDFMVDWWALGVCLFEFLTGVPPFNDETPQLVFQNILNRDIPWPDGEEe-LTLNSRNAIEILLT 798 zebrafish
NP_524860 704 LGTPDYLAPELLLKQgHGPAVDWWALGVCFYEFMTGIPPFNDETPQKVFDNILNKNIEWPEGDEa-LSVESMEAVELLLT 782 fruit fly
Q6NTJ3    746 LGTPDYLAPELLLRKsHGPAVDWWALGVCLFEFLTGIPPFNDETPSQVFQNILNRDIPWPEEEEevLSVNAQSAIEILLT 825 African clawed frog

Feature 1                                                          
Q96GX5    818 IDDTKRAGMKELKRHPLFSDVDWENLqhqTMPFIPQPDDETDTSYFEARNTAQ 870 human
E1C2I2    820 FDSTKRAGLKELKHHPLFHGVDWDNLqnqPMPFIPQPDDETDTSYFEARNNAQ 872 chicken
Q6DBX4    799 MDTLKRAGLKELKDHPFFDGVDWENLhhqTMPFIPQPDNETDTSYFEARNTAQ 851 zebrafish
NP_524860 783 MDPNERPAAKEVQQMRHFACIDWENIgntEPPFVPTPDNPTDTGYFDARNNLQ 835 fruit fly
Q6NTJ3    826 IDPTKRAGLKDLKAHPLFHGMEWEELqyqPMSFIPQPDDETDTTYFEARNNAQ 878 African clawed frog

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap