Conserved Protein Domain Family
PTS_IIB_bgl_like

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cd05568: PTS_IIB_bgl_like 
PTS_IIB_bgl_like: the PTS (phosphotransferase system) IIB domain of a family of sensory systems composed of a membrane-bound sugar-sensor (similar to BglF) and a transcription antiterminator (similar to BglG) which regulate expression of genes involved in sugar utilization. The domain architecture of the IIB-containing protein includes a region N-terminal to the IIB domain which is homologous to the BglG transcription antiterminator with an RNA-binding domain followed by two homologous domains, PRD1 and PRD2 (PTS Regulation Domains). C-terminal to the IIB domain is a domain similar to the PTS IIA domain. In this system, the BglG-like region and the IIB and IIA-like domains are all expressed together as a single multidomain protein. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include this sensory system with similarity to the bacterial bgl system, chitobiose/lichenan, ascorbate, lactose, galactitol, mannitol, and fructose systems.
Statistics
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PSSM-Id: 99910
View PSSM: cd05568
Aligned: 155 rows
Threshold Bit Score: 55.9727
Threshold Setting Gi: 26553899
Created: 11-May-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit, in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              # ##  ##                                                                  
NP_756350    436 KRVLLVCSTGIGTSHLLKSRILRAFPEWTIv-DVISAANLSqv---lpDNIELIISTINLptVTMPVAYVTAFFNDADIK 511 Escherichia col...
YP_001449737 393 NDIIIVCNEGISTSQMLNSRLQRVFAFNIL--GTFSKREALawq--qtHHADLIITTVDIpeARFPTIKVNALLLSEDIE 468 Streptococcus g...
NP_816628    396 MTAYFLFQGEPAWKAFLQQELAAYLGTRVKl-QAIEYVELSql---tlNEADIIISNFPLdhLDLPVFYLSLIPTKNELR 471 Enterococcus fa...
ZP_02077015  402 IYCFFLCENYHQVKQYLYNKLRKILPDTIQl-IQGTFENLQn------QSVDLVITTDNIvhLPIPVIEVSPFLTLADID 474 Eubacterium dol...
ZP_01802022  399 KNVVLVCGSGYGSSRLLEVQLNKVFHDKINilQCLSFNQFLas---elSDVDIIISTIPLnhDSIPIVLVDLKLLKKDIE 475 Clostridium dif...
NP_466306    404 QKVLIVCASGLGTSQLLESKVRKEFENTLEivGVYSFKEYEqs----gTMCDFVISTVPLrmKLHPFIQVSPFLTKADIR 479 Listeria monocy...
ZP_02025595  402 KNVILVCGSGHATTRMLEARLNVVFPDKINivKCVSYNEYSnytkenvKDIDFVITTVVLksNLLPSIMVDFALNNKDVE 481 Eubacterium ven...
ZP_01129326  393 LTAALVCPNYHDLHVILRERIETVLGDELHl-RVVITRTDVdl---aeLTTDLVLTTIDLrsFADNVVLIQPFLTEADVD 468 marine actinoba...
ZP_00604043  400 YQTVLVSEYSKAMATFLEARLNRELPELTIm-DLVEYKPELkrq--dfPSVDFILATLPFkhISIPVIEISPMITETDLA 476 Enterococcus fa...
NP_053208    397 KKVLLVTGESESWAEYLAATLAKRFGDKIQisTVFFAKKSDh-----dVNADFIISTIPLdlGSTPIICINSIPTERDYT 471 anthrax
Feature 1                 
NP_756350    512 RVTEMVITE 520 Escherichia coli CFT073
YP_001449737 469 RIQKISNQS 477 Streptococcus gordonii str. Challis substr. CH1
NP_816628    472 RLAELTLHS 480 Enterococcus faecalis V583
ZP_02077015  475 KINKVISNL 483 Eubacterium dolichum DSM 3991
ZP_01802022  476 NISKSITNN 484 Clostridium difficile QCD-32g58
NP_466306    480 NITALMKQD 488 Listeria monocytogenes EGD-e
ZP_02025595  482 SINRYLSKL 490 Eubacterium ventriosum ATCC 27560
ZP_01129326  469 SIRKTIGRV 477 marine actinobacterium PHSC20C1
ZP_00604043  477 YLTKYMLEH 485 Enterococcus faecium DO
NP_053208    472 NIQYYLDLQ 480 anthrax

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