1TVM


Conserved Protein Domain Family
PTS_IIB_galactitol

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cd05566: PTS_IIB_galactitol 
Click on image for an interactive view with Cn3D
PTS_IIB_galactitol: subunit IIB of enzyme II (EII) of the galactitol-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is a galactitol-specific permease with two cytoplasmic domains (IIA and IIB) and a transmembrane channel IIC domain that are expressed on three distinct polypeptide chains, in contrast to other PTS sugar transporters. The three genes encoding these subunits (gatA, gatB, and gatC) comprise the gatCBA operon. Galactitol PTS permease takes up exogenous galactitol, releasing the phosphate ester into the cytoplasm in preparation for oxidation and further metabolism via a modified glycolytic pathway called the tagatose-6-phosphate glycolytic pathway. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include galactitol, chitobiose/lichenan, ascorbate, lactose, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.
Statistics
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PSSM-Id: 99908
View PSSM: cd05566
Aligned: 40 rows
Threshold Bit Score: 83.3555
Threshold Setting Gi: 15900549
Created: 11-May-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit, in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              # ##  ##                                                                  
1TVM_A        22 RKIIVACGGAVATSTMAAEEIKELCQSHNI-PVELIQCRVNEiet--ymDGVHLICTTARVdr------sfGDIPLVHGM 92  Escherichia coli
AP_004795      2 KKILVACGTGMSTSTMIAHKLQEFLTEQGI-SATTAQCCLNEipl--ncNGMDLIVTSMRTns-------dYGIPTLNGA 71  Escherichia col...
YP_848549      4 IKVISVCGSGTVTSSMIAGKVKDVLADNGF-RATTVEVNPGGveqelssGNWDIIVHTSPLrg-------kYDIPTINAV 75  Listeria welshi...
YP_001307686   4 VNVLSVCGSGTVTSSMVAGKVKETLKEKGI-SSSTTEARPTEalnlaqsGRFDFIVYTSPLpp------gdYKIPTINAF 76  Clostridium bei...
ZP_02076851    2 KKVIVACGSGVATSQTVASKVNRLLKEKGV-DAVVEAVDLKSvdr--hlVDACAYITITKVsk-------eYDVPVINGI 71  Eubacterium dol...
EDP15294       2 KRIIVACANGVATSQAVASKVNRLLKERKV-DAVVDAVNIKSldr--diKDCVAYITITKTvk-------eYPVPVINGI 71  Clostridium bol...
NP_465621      2 KKVIVACGSGVATSQTVASKVERILKEKGV-RATVEAVDIKSleq--hiKTSDVYVAITKAnk-------eFDIPTLNGI 71  Listeria monocy...
ZP_00604035    3 NRLIVACGSGVATSQTIASKIQSMLEDDGI-AFPVEAVDYKSiqn--elLTAGIYVYVAQPdeevleqakdLGIEVFPGI 79  Enterococcus fa...
ZP_02076852    2 KRIIVACGNGVATSQAVAFRINRMLEKNHLyDVKVEAVNIRElhr--elKGAVAYVEVMKTde-------kCDIPKIDGL 72  Eubacterium dol...
NP_786799      2 AKIIVACGSGVASSELVVKHITDFFEDHHItGVEVEATDFKKlpd--ilANYDVYVWIARPneeiqtivdrYGIASVNGL 79  Lactobacillus p...
Feature 1                           
1TVM_A        93 PFVSGVGIEALQNKILTIL 111 Escherichia coli
AP_004795     72 ALLTGINDDALKQQIKALL 90  Escherichia coli W3110
YP_848549     76 GFLTGMDEEGFIEELLETA 94  Listeria welshimeri serovar 6b str. SLCC5334
YP_001307686  77 ACLTGIGEDKFFEDVFKTV 95  Clostridium beijerinckii NCIMB 8052
ZP_02076851   72 AFLTGMNQNKELERLIEAI 90  Eubacterium dolichum DSM 3991
EDP15294      72 AFLTGVRQEDELKKLIDAV 90  Clostridium bolteae ATCC BAA-613
NP_465621     72 AFLTGMGMDEETEKLLNAL 90  Listeria monocytogenes EGD-e
ZP_00604035   80 PFLTGMGVEPIYDSIKELV 98  Enterococcus faecium DO
ZP_02076852   73 VFLYGNANQKEEEFQRLLD 91  Eubacterium dolichum DSM 3991
NP_786799     80 PIFTGQSPETSYRDIITAL 98  Lactobacillus plantarum WCFS1

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