Conserved Protein Domain Family
PTS_IIB_ascorbate

?
cd05563: PTS_IIB_ascorbate 
PTS_IIB_ascorbate: subunit IIB of enzyme II (EII) of the L-ascorbate-specific phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). In this system, EII is an L-ascorbate-specific permease with two cytoplasmic subunits (IIA and IIB) and a transmembrane channel IIC subunit. Subunits IIA, IIB, and IIC are encoded by the sgaA, sgaB, and sgaT genes of the E. coli sgaTBA operon. In some bacteria, the IIB (SgaB) domain is fused C-terminal to the IIA (SgaT) domain. The IIB domain fold includes a central four-stranded parallel open twisted beta-sheet flanked by alpha-helices on both sides. The seven major PTS systems with this IIB fold include ascorbate, chitobiose/lichenan, lactose, galactitol, mannitol, fructose, and a sensory system with similarity to the bacterial bgl system.
Statistics
?
PSSM-Id: 99905
View PSSM: cd05563
Aligned: 54 rows
Threshold Bit Score: 63.6993
Threshold Setting Gi: 72080884
Created: 11-May-2007
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Feature 1:active site [active site]
Evidence:
  • Comment:most of the active site residues are contained within a P-loop which forms part of a binding pocket for the phosphoryl group
  • Comment:for the E coli chitobiose transporter IIB subunit,(not included in this IIB subgroup) in addition to the P-loop residues, two conserved residues are likely to be important in catalysis: a tyrosine whose side-chain points towards the phosphorylation site and a Gln residue whose side-chain lies close to the sulfur atom of the catalytic cysteine.

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1             # ##  ##                                                                   
P69822         4 RILAVCGNGQGSSMIMKMKVDQFLTQSNId---HTVNSCAVGEyks--elSGADIIIASTHIag--eiTVTGNKYVVGVR 76  Escherichia col...
ZP_00348270  386 RILAVCGSGQGSSMMMKMKIKQYLDKRGVp---NIMDSCAVTDhkg--kvGSVDIIVCSKHLqn--eiVANEHISVLGVQ 458 Actinobacillus ...
EDK31053     510 RVLVCCGSGQGSSMMCSKKIKEFLDKKGIpattFNSAITDYKSh-----lGSFDIIVTSVALaek-itNLPEGKQQLAVK 583 Vibrionales bac...
NP_814858      2 RILVSCANGSGTSLMMMRSVEKAMKELGVp--iTKIHHCAISEgks--saSQYDVVFTPVNFlqmfqqAEKRGVTVIGIK 77  Enterococcus fa...
YP_807892      2 KILVSCANGSGTSLMMMRSVQKAFKRLNIp--iTQIEHTNLAEgks--taKQYDMVFTTTNFvdmfkdAQSKGVQVIGVK 77  Lactobacillus c...
YP_278800      2 KILCICGSGMGTSMIIKMKANQALKELNLe---GSVESIGLGQgkt--vaINFDVIFCTQNFvd---eIPKGKTLVYGIN 73  Mycoplasma syno...
NP_720732      3 KVLTACGNGMGSSMVIKMKVENALRQLGV----SDIESASCSVgeakglaSNYDIVVASNHLih--elDGRTNGKLIGLD 76  Streptococcus m...
YP_115898      4 KIVAACGNGMGTSMIIKLKVQKIVKELGId---ATVEALSMGQskg--ltNSVDIIIASKHLvs--efSQKQKAKIVGVT 76  Mycoplasma hyop...
YP_001256776   2 KVLCLCGSGMGTSMIIKLKTEQAMRELGIq---GSVEALGLGMgks--vaNNFDVILCTNNFvs---eVANSKASVHGLK 73  Mycoplasma agal...
YP_001449738   3 KIVTVCGAGVGSSMMERLFAQQILDAENIe---AEIDASDIGSvd----pNSYDIVITTSDFan---qLAHAQSKVIRID 72  Streptococcus g...
Feature 1                        
P69822        77 NMLSPADFGPKLLEVI 92  Escherichia coli K12
ZP_00348270  459 NMLNPNTFGEELLALI 474 Actinobacillus pleuropneumoniae serovar 1 str. 4074
EDK31053     584 NMIMVKTFGDELISLI 599 Vibrionales bacterium SWAT-3
NP_814858     78 NVMSAKEIQEKYMASD 93  Enterococcus faecalis V583
YP_807892     78 NVMSDKEVEQHVREDT 93  Lactobacillus casei ATCC 334
YP_278800     74 NVMDLNEIKSKILEAK 89  Mycoplasma synoviae 53
NP_720732     77 NLMDDNEIKTKLEEAL 92  Streptococcus mutans UA159
YP_115898     77 NLMDENEIKTALTPVL 92  Mycoplasma hyopneumoniae 232
YP_001256776  74 NVMDINEIKAAFKDAL 89  Mycoplasma agalactiae PG2
YP_001449738  73 NLMDKEYLKEQLLATI 88  Streptococcus gordonii str. Challis substr. CH1

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap