1TZS


Conserved Protein Domain Family
Cathespin_E

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cd05486: Cathespin_E 
Click on image for an interactive view with Cn3D
Cathepsin E, non-lysosomal aspartic protease.
Cathepsin E is an intracellular, non-lysosomal aspartic protease expressed in a variety of cells and tissues. The protease has proposed physiological roles in antigen presentation by the MHC class II system, in the biogenesis of the vasoconstrictor peptide endothelin, and in neurodegeneration associated with brain ischemia and aging. Cathepsin E is the only A1 aspartic protease that exists as a homodimer with a disulfide bridge linking the two monomers. Like many other aspartic proteases, it is synthesized as a zymogen which is catalytically inactive towards its natural substrates at neutral pH and which auto-activates in an acidic environment. The overall structure follows the general fold of aspartic proteases of the A1 family, it is composed of two structurally similar beta barrel lobes, each lobe contributing an aspartic acid residue to form a catalytic dyad that acts to cleave the substrate peptide bond. The catalytic Asp residues are contained in an Asp-Thr-Gly-Ser/thr motif in both N- and C-terminal lobes of the enzyme. The aspartic acid residues act together to allow a water molecule to attack the peptide bond. One aspartic acid residue (in its deprotonated form) activates the attacking water molecule, whereas the other aspartic acid residue (in its protonated form) polarizes the peptide carbonyl, increasing its susceptibility to attack. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133153
View PSSM: cd05486
Aligned: 4 rows
Threshold Bit Score: 596.481
Threshold Setting Gi: 46395759
Created: 9-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:The two ASPs at the active site plays key catalytic roles in the pepsin family and conserved for all family members.
  • Comment:Predicted based on homologous proteins woth structures.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #                                                             
1TZS_A        25 YFGTISIGSPPQNFTVIFDTGSSNLWVPSVYCTSPACKTHSRFQPsqSSTYSQPGQSFSIQYGTGSLSGIIGaDQVSVEG 104 human
XP_001235024  79 YFGQISIGTPPQNFTVVFDTGSSNLWVPSIYCTSKACTKHARFQPshSSTYQPLGIPVSIQYGTGSLTGIIGsDQVTVEG 158 chicken
Q805F3        74 YFGEISVGTPPQNFTVIFDTGSSNLWVPSVYCISQACAQHDRFQPqlSSTYESNGNNFSLQYGTGSLSGVIGiDAVTVEG 153 African clawed ...
Q800A0        74 YFGQISIGTPPQQFTVIFDTGSSNLWVPSIYCTSQACTKHNRYRPseSTTYVSNGEAFFIQYGTGNLTGILGiDQVTVQG 153 bullfrog
Feature 1                                                                                        
1TZS_A       105 lTVVGQQFGESVTEPGqTFVDAEFDGILGLGYPSLAVggvTPVFDNMMAQNLVDLPMFSVYMSSNPeggaGSELIFGGYD 184 human
XP_001235024 159 mTVYNQPFAESVSEPGkTFQDSEFDGILGLAYPSLAVdgvTPVFDNMMAQDLVEMPIFSVYMSANPdsslGGEVLFGGFD 238 chicken
Q805F3       154 iLVQNQQFGESVSEPGsTFVDAEFDGILGLGYPSIAVgdcTPVFDNMIAQNLVELPMFSVYMSRNPnsavGGELVFGGFD 233 African clawed ...
Q800A0       154 iTVQSQTFAESVSEPGsTFQDSNFDGILGLAYPNLAVdncIPVFDNMIAQNLVELPLFGVYMNRDPnsadGGELVLGGFD 233 bullfrog
Feature 1                                                   #                                    
1TZS_A       185 HSHFsGSLNWVPVTkqaYWQIALDNIQVGGTVMFCseGCQAIVDTGTSLITGPSDKIKQLQNAIGAapvDGEYAVECANL 264 human
XP_001235024 239 PSRFlGTLHWVPVTqqgYWQIQLDNVQVGGTVAFCadGCQAIVDTGTSLLTGPTKDIKEMQRYIGAtamDGEYIVDCGRL 318 chicken
Q805F3       234 ASRFsGQLNWVPVTnqgYWQIQLDNVQINGEVLFCsgGCQAIVDTGTSLITGPSSDIVQLQNIIGAsaaNGDYEVDCSVL 313 African clawed ...
Q800A0       234 TSRFsGQLNWVPITvqgYWQIQVDSIQVAGQVIFCsdGCQAIVDTGTSLITGPSGDIEQLQNYIGVtntNGEYGVSCSTL 313 bullfrog
Feature 1                                                                                    
1TZS_A       265 NVMPDVTFTINGVPYTLSPTAYtlldfvdgmqfCSSGFQGLDIHPPAGPLWILGDVFIRQFYSVFDRGNNRVGLAP 340 human
XP_001235024 319 SSMPIVTFTINGIPYVLSAQAYtlmeqsdgvdiCLSGFQGMDVPPPAGPLWILGDVFIRQYYSVFDRGNNRVGFAP 394 chicken
Q805F3       314 NEMPTVTFTINGIGYQMTPQQYtlqd---gggvCSSGFQGLDIPPPAGPLWILGDVFIGQYYSVFDRGNNRVGLAP 386 African clawed frog
Q800A0       314 SLMPSVTFTINGLDYSLTPEQYmled---gggyCSSGFQGLDISPPSGPLWILGDVFIGQYYSVFDRGNNRVGFAP 386 bullfrog

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