1HTR


Conserved Protein Domain Family
gastricsin

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cd05477: gastricsin 
Click on image for an interactive view with Cn3D
Gastricsins, asparate proteases produced in gastric mucosa.
Gastricsin is also called pepsinogen C. Gastricsins are produced in gastric mucosa of mammals. It is synthesized by the chief cells in the stomach as an inactive zymogen. It is self-converted to a mature enzyme under acidic conditions. Human gastricsin is distributed throughout all parts of the stomach. Gastricsin is synthesized as an inactive progastricsin that has an approximately 40 residue prosequence. It is self-converting to a mature enzyme being triggered by a drop in pH from neutrality to acidic conditions. Like other aspartic proteases, gastricsin are characterized by two catalytic aspartic residues at the active site, and display optimal activity at acidic pH. Mature enzyme has a pseudo-2-fold symmetry that passes through the active site between the catalytic aspartate residues. Structurally, aspartic proteases are bilobal enzymes, each lobe contributing a catalytic aspartate residue, with an extended active site cleft localized between the two lobes of the molecule. One lobe may be evolved from the other through ancient gene-duplication event. Although the three-dimensional structures of the two lobes are very similar, the amino acid sequences are more divergent, except for the conserved catalytic site motif. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133144
View PSSM: cd05477
Aligned: 4 rows
Threshold Bit Score: 561.817
Threshold Setting Gi: 999902
Created: 14-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Citation:PMID 7714902
  • Comment:The two ASPs at the active site plays key catalytic roles in the pepsin family and conserved for all family members.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #                                                          
1HTR_B     11 DAAYFGEISIGTPPQNFLVLFDTGSSNLWVPSVYCQsQACTSHSRFNPSeSSTYSTNGQTFSLQYGSGSLTGFFGYDTLT 90  human
BAA76892   70 DMSYYGEISIGTPPQNFLVLFDTGSSNLWVPSTLCQsQACANHNEFDPNeSSTFSTQDEFFSLQYGSGSLTGIFGFDTVT 149 chicken
BAB20797   64 DMSYYGEISIGTPPQNFLVLFDTGSSNLWVASTYCQsQACTNHPLFNPSqSSTYSSNQQQFSLQYGTGSLTGILGYDTVT 143 African clawed frog
AAG35646   66 DTTYYGAITIGTPPQSFQVLFDTGSANLWVDSVLCNtQACNTHTKFNPQqSSTYSANGETFYLPYGAGSLSGVFGYDTVN 145 brook trout
Feature 1                                                                                     
1HTR_B     91 VqsiqvpNQEFGLSENEPGtnfvyaQFDGIMGLAYPalsvdeaTTAMQGMVQEGALtspVFSVYLsnqqgssGGAVVFGG 170 human
BAA76892  150 IqgisitNQEFGLSETEPGtsflysPFDGILGLAFPsisaggaTTVMQKMLQENLLdfpVFSFYLsgqegsqGGELVFGG 229 chicken
BAB20797  144 IqnvaisQQEFGLSETEPGtnfvyaQFDGILGLAYPsiavggaTTVMQGMMQQNLLnqpIFGFYLsgqssqnGGEVAFGG 223 African clawed frog
AAG35646  146 VggiiltNQEIGLSTDEPGqnfvvaQFDGILGLSYPsisagqeTPVMDNMMSQNLLqanIFAFYMtrd-gqqGSELSFGE 224 brook trout
Feature 1                                                   #                                 
1HTR_B    171 VDSslYTGQIYWAPVTqelYWQIGIeefligGQASGWCseGCQAIVDTGTSLLTVPqqymsallqatGAQEDeygqflvn 250 human
BAA76892  230 VDPnlYTGQITWTPVTqttYWQIGIedfavgGQSSGWCsqGCQGIVDTGTSLLTVPnqvftelmqyiGAQADdsgqyvas 309 chicken
BAB20797  224 VDQnyYTGQIYWTPVTsetYWQIGIqgfsinGQATGWCsqGCQAIVDTGTSLLTAPqsvfssliqsiGAQQDqngqyvvs 303 African clawed frog
AAG35646  225 VDNtkYQGQIYWTPVTsqtYWQIGIqgfqinGQETGWCgqGCQAIVDTGTSMLTAPrqimgtlmqsiGAQQDqygqytvn 304 brook trout
Feature 1                                                                                    
1HTR_B    251 cnsiqnLPSLTFIINgVEFPLPPSSYIlsn-ngyCTVGVEPTylssqngqpLWILGDVFLRSYYSVYDLgnNRVGFATA 328 human
BAA76892  310 csnieyMPTITFVISgTSFPLPPSAYMlqsnsdyCTVGIESTylpsqtgqpLWILGDVFLRVYYSIYDMgnNQVGFATA 388 chicken
BAB20797  304 csniqnLPTISFTISgVSFPLPPSAYVlqqssgyCTIGIMPTylpsqngqpLWILGDVFLREYYSVYDLgnNQVGFATA 382 African clawed frog
AAG35646  305 cnqinsLPTLTFTINgINFPLPPSAYIqqn-nqvCSVGITPTylpsqngqpLWILGDVFLMQYYSVYDRtsNQVGFAPA 382 brook trout

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