1ZAP,2H6T,1J71,1EAG,2H6S


Conserved Protein Domain Family
SAP_like

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cd05474: SAP_like 
Click on image for an interactive view with Cn3D
SAPs, pepsin-like proteinases secreted from pathogens to degrade host proteins.
SAPs (Secreted aspartic proteinases) are secreted from a group of pathogenic fungi, predominantly Candida species. They are secreted from the pathogen to degrade host proteins. SAP is one of the most significant extracellular hydrolytic enzymes produced by C. albicans. SAP proteins, encoded by a family of 10 SAP genes. All 10 SAP genes of C. albicans encode preproenzymes, approximately 60 amino acid longer than the mature enzyme, which are processed when transported via the secretory pathway. The mature enzymes contain sequence motifs typical for all aspartyl proteinases, including the two conserved aspartate residues other active site and conserved cysteine residues implicated in the maintenance of the three-dimensional structure. Most Sap proteins contain putative N-glycosylation sites, but it remains to be determined which Sap proteins are glycosylated. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA). The overall structure of Sap protein conforms to the classical aspartic proteinase fold typified by pepsin. SAP is a bilobal enzyme, each lobe contributing a catalytic Asp residue, with an extended active site cleft localized between the two lobes of the molecule. One lobe may be evolved from the other through ancient gene-duplication event. More recently evolved enzymes have similar three-dimensional structures, however their amino acid sequences are more divergent except for the conserved catalytic site motif. This family of aspartate proteases is classified by MEROPS as the peptidase family A1 (pepsin A, clan AA).
Statistics
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PSSM-Id: 133141
View PSSM: cd05474
Aligned: 93 rows
Threshold Bit Score: 226.678
Threshold Setting Gi: 50550411
Created: 7-Sep-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic residue [active site]
Evidence:
  • Comment:The two ASPs at the active site plays key catalytic roles in the pepsin family and conserved for all family members.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #                                                           
1ZAP_A     12 VTYAADITVGsnnqKLNVIVDTGSSDLWVPdvnidcqvtysdqtadfckqkgtydpsgssasqdlntPFSIGYGDgSSSQ 91  Candida albicans
XP_500342  59 YWYSVALQLGspaqNFNVLLDTGSSDLWVYasndtqdcennacaft-----gtfnadqsstyqflndDFSIHYVS-GSSV 132 Yarrowia lipolytic...
XP_501124  57 YYYSVALSLGtpaqDFNVILDTGSSDLWVFasnatqscenhacdft-----gqfnaldsssyhfignNYSIEYVSgDNAT 131 Yarrowia lipolytic...
2H6T_A     12 VSYASDITVGsnkqKLTVVIDTGSSDLWVPdsqvscqagqgqdpnfck-negtyspssssssqnlnsPFSIEYGDgTTSQ 90  Candida albicans
XP_502045  60 QYYSIDLTLGtpaqKFNVLLDTGSSDLWVYnvsdttdcaggacknt-----gtfdssksstyhsiddKYFIQYVS-GNAS 133 Yarrowia lipolytic...
XP_501619  47 TYYRAKVSLGtpaqVFNVIFDTGSSDLWVPnydskass----------------------sykylnsDFNVSYTAvNGIL 104 Yarrowia lipolytic...
XP_503918  47 VYISTYISVGtppqSFEVSFDTGSSDLWIPavgddqtggpglf-----------npqasstfkslgtSFNAAYVG-GTAQ 114 Yarrowia lipolytic...
XP_501592  43 RYLYTNITLGtppqKFEVTFDTGSADLWVPtapdqqcsgqcpatag------afdadasstysllnnNFDVGYIGdPNNR 116 Yarrowia lipolytic...
XP_502672 409 LYYFVDAYLGtpgqKNSLLVDTGSSDLWVIdeangghfnkn----------------dsstwkhlnwDFKNAYQA-GQIS 471 Yarrowia lipolytic...
XP_504725  58 MAYNVDIKVGg--qKVTVQIDTGSSDLWVFssdsiycendpslctky----gsynpkksqnskdtgeHFDIQYGI-GDAS 130 Yarrowia lipolytic...
Feature 1                                                                                     
1ZAP_A     92 GTLyKDTVGFGgvsIKNQVLADvds-----tsiDQGILGVGYKtnea-----ggsYDNVPVTLKKQGVIAKNAYSLYLNs 161 Candida albicans
XP_500342 133 GNWgTDTLHIGgvsLTDFQFAAaas-----aggGQGVLGISLQgsesv--ppgnqYENFPLKLKSDGYIDRAVFSLYLDd 205 Yarrowia lipolytic...
XP_501124 132 GNWgTDTLSIGsvkLENLQFAAvth-----tsaQSGILGISLQgsesvv-fvkneYPNFPVQLVNQGWIDRTVYSLYLNs 205 Yarrowia lipolytic...
2H6T_A     91 GTWyKDTIGFGgisITKQQFADvts-----tsvDQGILGIGYKthea-----egnYDNVPVTLKNQGIISKNAYSLYLNs 160 Candida albicans
XP_502045 134 GNWgTDTLSLEgvsLPDFRFATidn-----aqgNTGILGVSLPgsesvr-kgestYDNFPIALQKAGYIDRRVYSLYLNd 207 Yarrowia lipolytic...
XP_501619 105 GDWaTETLKLGpvtLENFQFGNvkg-----nhaGGGVFGVAFRaqemv--kpgdyYDNFPYAVKKAGYINKAAYSVFLDd 177 Yarrowia lipolytic...
XP_503918 115 GYWvTDNVNAAglsLSNLQLGAvsnm----qdqKQGILGVSFKqvemsvrngqpqYYNFPFSAKEQGFIDHVLYSLHFDg 190 Yarrowia lipolytic...
XP_501592 117 GNWvSDTLTVGsvtLPQFSFGTvq-------teDGGIFGVSFEeqessa-ddgetYPNFVFASKQQGYIDRAVFSIFSPs 188 Yarrowia lipolytic...
XP_502672 472 GEWgTDSVTIDnatVHDLVMGVantsaltsgdnVMGLLGLGFEglegtqqtdigqYKNYPRRLVDSGLTDVAAYSVWLNs 551 Yarrowia lipolytic...
XP_504725 131 GNYyKDDASLGeakVTDFQFGVstgd---kssgDVSVFGIGPLadea----trshYPNFPALLKEQGVIKKNVYGMAFGl 203 Yarrowia lipolytic...
Feature 1                                                                          #          
1ZAP_A    162 pd-saTGQIIFGGVDnAKYSGSLIALPVTSD--------RELRISLGSVEvsgkti----ntdnvDVLLDSGTTITYLQQ 228 Candida albicans
XP_500342 206 ls-ssTGNLLFGGVDkAKYEGDLAVLPLTSS--------AAFQVAYSGISvgqns------ygggNAVLDSGTSYTYIPD 270 Yarrowia lipolytic...
XP_501124 206 in-atEGTLLLGGIDtAKYSGHLEVLDLADH--------DDFSVPYSDVSwngns-----ygrasAAVFDSGTSYIYMPN 271 Yarrowia lipolytic...
2H6T_A    161 rq-atSGQIIFGGVDnAKYSGTLIALPVTSD--------NELRIHLNTVKvagqs-----inadvDVLLDSGTTITYLQQ 226 Candida albicans
XP_502045 208 tn-afNGTFLLGGIDhAKFNGSLTVLPLPSP--------DEFAVKYENIIldgkp-----iakpgYTVLDSGTSFSYIPE 273 Yarrowia lipolytic...
XP_501619 178 pd-tnEATFLLAGVDhAKFEGDLSWLNVSDPa-------AGALVQLKDISlgskh-----mtvdaPVVLDTGTIFTSVPD 244 Yarrowia lipolytic...
XP_503918 191 ph-spDGTFLLGGIDhAKYSGDLHYYNVANPs-------AGPQINFKSLVldghe-----ldfnlPVTLDSGSLAIAFPD 257 Yarrowia lipolytic...
XP_501592 189 as-ssEVTFLLGGIDhAKFSGDLAWNKVPDPsrgasilvDDFSVNGVNFSly------------sSFAVDTGSVATLIPD 255 Yarrowia lipolytic...
XP_502672 552 rq-tgQGNFLFGGVDtAKYEGELQTIDMPDVvvg--nmrGYLTTHMTNIAinnpngsvsnlpkdlYAMFDTGAPSTLLPD 628 Yarrowia lipolytic...
XP_504725 204 pgdkqNSEITFGAYNsGRYSGSLKTVPITTQ--------GHFGIKCDKAKigdkt-----ilegeDVVLDSGTSLTYLTK 270 Yarrowia lipolytic...
Feature 1                                                                                     
1ZAP_A    229 DLADQIIKAFNgkltqdsn-gnsfyevDCNLsg--dVVFNFSKn------AKISVPASDFAastqgdd-gqpydkCQLLF 298 Candida albicans
XP_500342 271 QDFQQLAQDLNfqetdqs---tglpiiDCDSne--pVTFSFDG-------KDIVVPSSQMViplstlvgddsetqCVFGI 338 Yarrowia lipolytic...
XP_501124 272 DVFYGLSSDINlnettna--dtnlqyiACDAdi--nVVVTFGN-------TDITLGADQLVlhlsellddktqteCVFGI 340 Yarrowia lipolytic...
2H6T_A    227 GVADQVISAFNgqetydan-gnlfylvDCNLsg--sVDFAFDKn------AKISVPASEFTaplyted-gqvydqCQLLF 296 Candida albicans
XP_502045 274 TVYNTIAKKHNlgdvtf----fglpsiDCKAnv--sLDFEFDG-------VTIHAQREQLIidpg-------igfCFFGV 333 Yarrowia lipolytic...
XP_501619 245 PIYQELKKTLNlgkfnkf---lginyiDCDAkm--sVAFNFPGatvladeKSLVVPIGPFYgnd--------dkrCGFGI 311 Yarrowia lipolytic...
XP_503918 258 TQFRAIGSGLNltnynkd---mglyyiDCQAei--sVDFKFDG-------LTISANSSSLVlpmgffsgdttdpsCVLGV 325 Yarrowia lipolytic...
XP_501592 256 DVFQHLTRTVQvgswipd---lgvytiDCNAep--sISFNLPNgtihahpETMVVPISRLSgnqs-------dtgCYFGL 323 Yarrowia lipolytic...
XP_502672 629 DVGLAFKDQFNlsynsa----wglyygDCNQgg--tFTFTLGG-------KDFTMDVKDFVvdwvsa--tipegqCFFGI 693 Yarrowia lipolytic...
XP_504725 271 SNYDAVINQVQaagikltdagqgisalPCSDigklsMEYTFSG-------KTIKVSGKDMTipatyldssdtsglCIFGI 343 Yarrowia lipolytic...
Feature 1                                           
1ZAP_A    299 DVNk------ANILGDNFLRSAYIVYDLDDNEISIAQV 330 Candida albicans
XP_500342 339 QSAast--qdYVLFGDTFLRAAYVVYDLDQEQVGIAQA 374 Yarrowia lipolytica CLIB122
XP_501124 341 QSTdys--sdNILFGDVFLRSVYAVYDLDNYQIGVAQA 376 Yarrowia lipolytica CLIB122
2H6T_A    297 GTSd------YNILGDNFLRSAYIVYDLDDNEISLAQV 328 Candida albicans
XP_502045 334 LPStys--qnITLFGDTFLRNAYVVYDLEDLQIGIAQA 369 Yarrowia lipolytica CLIB122
XP_501619 312 QSTtvf--ggDPIMGDTVMRAAYVVYDLEDHRCGVAQA 347 Yarrowia lipolytica CLIB122
XP_503918 326 QNSalyespnDAVLGEPFLKNAYVVYDLEDYSIGLAPA 363 Yarrowia lipolytica CLIB122
XP_501592 324 VSSety--dgISILGDSFLRNAYAVFDLEDYKLGFAQA 359 Yarrowia lipolytica CLIB122
XP_502672 694 GPTrg----kMAMFGDTFLRSFYAVFDLDNRKASLAKA 727 Yarrowia lipolytica CLIB122
XP_504725 344 TEStgq-lsnLNLFGDTFLRAIYSVYDLDNNQVSIAQA 380 Yarrowia lipolytica CLIB122

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