1HRD,1BGV,2BMA


Conserved Protein Domain Family
NAD_bind_2_Glu_DH

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cd05313: NAD_bind_2_Glu_DH 
Click on image for an interactive view with Cn3D
NAD(P) binding domain of glutamate dehydrogenase, subgroup 2
Amino acid dehydrogenase (DH) is a widely distributed family of enzymes that catalyzes the oxidative deamination of an amino acid to its keto acid and ammonia with concomitant reduction of NADP+. Glutamate DH is a multidomain enzyme that catalyzes the reaction from glutamate to 2-oxyoglutarate and ammonia in the presence of NAD or NADP. It is present in all organisms. Enzymes involved in ammonia asimilation are typically NADP+-dependent, while those involved in glutamate catabolism are generally NAD+-dependent. Amino acid DH-like NAD(P)-binding domains are members of the Rossmann fold superfamily and include glutamate, leucine, and phenylalanine DHs, methylene tetrahydrofolate DH, methylene-tetrahydromethanopterin DH, methylene-tetrahydropholate DH/cyclohydrolase, Shikimate DH-like proteins, malate oxidoreductases, and glutamyl tRNA reductase. Amino acid DHs catalyze the deamination of amino acids to keto acids with NAD(P)+ as a cofactor. The NAD(P)-binding Rossmann fold superfamily includes a wide variety of protein families including NAD(P)- binding domains of alcohol DHs, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate DH, lactate/malate DHs, formate/glycerate DHs, siroheme synthases, 6-phosphogluconate DH, amino acid DHs, repressor rex, NAD-binding potassium channel domain, CoA-binding, and ornithine cyclodeaminase-like domains. These domains have an alpha -beta-alpha configuration. NAD binding involves numerous hydrogen and van der Waals contacts.
Statistics
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PSSM-Id: 133455
View PSSM: cd05313
Aligned: 27 rows
Threshold Bit Score: 369.253
Threshold Setting Gi: 30695050
Created: 21-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                    ###                   ##         
1HRD_A    193 TGKARSFGGSLVRPEATGYGSVYYVEAVMKHen-dtLVGKTVALAGFGNVAWGAAKKLAELGAKAVTLSGPdGYIYDpeg 271 Clostridium symbiosum
2BMA_F    215 TGKNVKWGGSNLRVEATGYGLVYFVLEVLKSln-ipVEKQTAVVSGSGNVALYCVQKLLHLNVKVLTLSDSnGYVYEpng 293 malaria parasite P...
P28998    269 TGKGQEYGGSEIRPEATGYGAVLFVENVLKDkg-esLKGKRCLVSGAGNVAQYCAELLLEKGAIVLSLSDSqGYVYEpng 347 Chlorella sorokiniana
NP_345769 196 TGKPLGFGGSLIRPEATGYGLVYYTEEMLKAng-nsFAGKKVVISGSGNVAQYALQKATELGATVISVSDSnGYVIDen- 273 Streptococcus pneu...
P31026    196 TGKGLTWGGSLVRTEATGYGCVYFVSEMIKAkg-esISGQKIIVSGSGNVATYAIEKAQELGATVIGFSDSsGWVHTpn- 273 Corynebacterium gl...
NP_175583 380 TGPRIYWAASSLRTEASGYGVVYFARLILADmn-keIKGLRCVVSGCGKIAMHVVEKLIACGAHPVTVSDSkGYLVDddg 458 thale cress
AAP83848  222 TGKGPTFGGSLIRPESTGYGVVMFVREMLKVkg-dtLEGKVVVISGSGNVAQYATQKVNALGGKVIALSDSkGVLVDeeg 300 Porphyra yezoensis
YP_448430 193 TGKGLTFGGSLARTEATGYGLLYFTNEMLKAnn-etLKDKTATVSGSGNVAIYAIEKAQQLGAKVVTASDStGWVYDpk- 270 Methanosphaera sta...
YP_590019 206 TGKGLNWGGSLIRPEATGYGCVYFAEEMLKTvk-qtLEGKVCLVSGSGNVSQYTIEKLLDLGAKPIACSDSnGIIHDpag 284 Acidobacteria bact...
CAJ20457  232 TGKDKNWGGSAMRPEATGYGCVYFVLELLKAqnkegIKGMRCAISGSGNVALYAGEKLATLGAKVMTFSDSsGYIVNekg 311 Toxoplasma gondii RH
Feature 1                                                                           ##        
1HRD_A    272 itteEKINYMLEMRASGRnk------------vqDYADKfg-------vqFFPGEKPWGQK--VDIIMPCATQNDVDLEQ 330 Clostridium symbiosum
2BMA_F    294 -fthENLEFLIDLKEEKKgr------------ikEYLNHsst------akYFPNEKPWGVP--CTLAFPCATQNDVDLDQ 352 malaria parasite P...
P28998    348 -ftrEQLQAVQDMKKKNNsar-----------isEYKSDta-------vyVGDRRKPWELDcqVDIAFPCATQNEIDEHD 408 Chlorella sorokiniana
NP_345769 274 ---gIDFDLLVDVKEKRRar------------ltEYAAEka-------taTYHEGTVWTYAgnYDIALPCATQNEINGEA 331 Streptococcus pneu...
P31026    274 ---gVDVAKLREIKEVRRar------------vsVYADEveg------atYHTDGSIWDLK--CDIALPCATQNELNGEN 330 Corynebacterium gl...
NP_175583 459 -fdyMKLAFLRDIKSQQRsl-------------rDYSKTyar------akYFDELKPWNER--CDVAFPCASQNEVDQAD 516 thale cress
AAP83848  301 -itdKKLAEVMDIKNVRRtslkaftekfstakwtDFSEQyt-------deDEKSNPLWEIK--CDVALPCATQNELNADG 370 Porphyra yezoensis
YP_448430 271 ---gIDVALLKEIKEVQRgr------------msDYAAKres------aeYHEGRGVWQIK--SDVALPCATQNELTLDD 327 Methanosphaera sta...
YP_590019 285 -idrEKLAYILDLKNTKRgr------------isDYAEKfktakyipvdpKLDHNPLWDIK--ADCAFPSATQNEINAKD 349 Acidobacteria bact...
CAJ20457  312 -fplGQIQRLKEMKETRSstr-----------vsEFAAKyst------vkFVPDKRAWEVP--CDIAFPCATQNEISEED 371 Toxoplasma gondii RH
Feature 1                   ###                                                               
1HRD_A    331 AKKIVANNVKYYIEVANMPTTNEALRFLMQQpnMVVAPSKAVNAGGVLVSGFEMSQNSERLSWTAEEVDSKLHQVMTDIH 410 Clostridium symbiosum
2BMA_F    353 AKLLQKNGCILVGEGANMPSTVDAINLFKSNn-IIYCPSKAANAGGVAISGLEMSQNFQFSHWTRETVDEKLKEIMRNIF 431 malaria parasite P...
P28998    409 AELLIKHGCQYVVEGANMPSTNEAIHKYNKAg-IIYCPGKAANAGGVAVSGLEMTQNRMSLNWTREEVRDKLERIMKDIY 487 Chlorella sorokiniana
NP_345769 332 AKRLVAQGVICVSEGANMPSDLDAIKVYKENg-IFYGPAKAANAGGVAVSALEMSQNSLRLSWTREEVDGRLKDIMTNIF 410 Streptococcus pneu...
P31026    331 AKTLADNGCRFVAEGANMPSTPEAVEVFRERd-IRFGPGKAANAGGVATSALEMQQNASRDSWSFEYTDERLQVIMKNIF 409 Corynebacterium gl...
NP_175583 517 AINLVNAGCRLLVEGSNMPCTAEAVDVFRKAn-VLIAPAIAAGAGGVAAGEIEVLRESNSMQWSAEDFESRLQEALKQTY 595 thale cress
AAP83848  371 ARALVDNGVSIVAEGANMPSTPGAIEVLRSAk-VAFAPGKASNAGGVATSGLEMSQNSQRMAWSAEKVEEELDGIMTRIH 449 Porphyra yezoensis
YP_448430 328 AKLIVENDTLAVSEGANMPTTLDATKYLQDNg-ILFGPAKAANAGGVAVSALEMSQNSERLYWTFDEVDNMLKDIMTNIF 406 Methanosphaera sta...
YP_590019 350 AKNLIKNGCKVVSEGANMPSTLEATNLFLEAg-VLYGPAKAANAGGVATSGLEMAQNSARLAWTREEVDHRLHNIMIAIH 428 Acidobacteria bact...
CAJ20457  372 AQLLIDNGCRIVAEGANMPTTRDAIRLFKQHg-VILCPGKAANAGGVAVSGLEMSQNAMRIEWTREVVDEKLRQIMASIF 450 Toxoplasma gondii RH
Feature 1                                              
1HRD_A    411 DGSAAAAERYgl----gyNLVAGANIVGFQKIADAMMAQGI 447 Clostridium symbiosum
2BMA_F    432 IACSENALKYtkn---kyDLQAGANIAGFLKVAESYIEQGC 469 malaria parasite P. falciparum
P28998    488 DSAMGPSREYn------vDLAAGANIAGFTKVADAVKAQGA 522 Chlorella sorokiniana
NP_345769 411 NTAKTTSETYgl----dkDYLAGANIAAFENVANAMIAQGI 447 Streptococcus pneumoniae TIGR4
P31026    410 KTCAETAAEYgh----enDYVVGANIAGFKKVADAMLAQGV 446 Corynebacterium glutamicum
NP_175583 596 EKALKAANDFgyqkespeALLHGATIAAFLNIAQAMTDQGC 636 thale cress
AAP83848  450 ADCVETAKAYdr----agDYVFGANVTGFLKVAEAMLAQGL 486 Porphyra yezoensis
YP_448430 407 SKLNTTAQKYeq----dkNYVLGANVAGFERVVEAMQSQGI 443 Methanosphaera stadtmanae DSM 3091
YP_590019 429 KNCFETAEKYgk----pgNLVVGANIGGFLKIADSMLDQGL 465 Acidobacteria bacterium Ellin345
CAJ20457  451 SKCRSYAAKFgen---efDLVTGANVAGFVKVADCVIDQGY 488 Toxoplasma gondii RH

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