1HYH,1EZ4,1LDN,1LLC


Conserved Protein Domain Family
HicDH_like

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cd05291: HicDH_like 
Click on image for an interactive view with Cn3D
L-2-hydroxyisocapronate dehydrogenases and some bacterial L-lactate dehydrogenases
L-2-hydroxyisocapronate dehydrogenase (HicDH) catalyzes the conversion of a variety of 2-oxo carboxylic acids with medium-sized aliphatic or aromatic side chains. This subfamily is composed of HicDHs and some bacterial L-lactate dehydrogenases (LDH). LDHs catalyze the last step of glycolysis in which pyruvate is converted to L-lactate. Bacterial LDHs can be non-allosteric or may be activated by an allosteric effector such as fructose-1,6-bisphosphate. Members of this subfamily with known structures such as the HicDH of Lactobacillus confusus, the non-allosteric LDH of Lactobacillus pentosus, and the allosteric LDH of Bacillus stearothermophilus, show that they exist as homotetramers. The HicDH-like subfamily is part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.
Statistics
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PSSM-Id: 133427
View PSSM: cd05291
Aligned: 66 rows
Threshold Bit Score: 372.185
Threshold Setting Gi: 71851261
Created: 14-Dec-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:1HYH_C; Weissella confusa Hydroxyisocaproate Dehydrogenase contacts to NAD at 3.5A
    View structure with Cn3D
  • Citation:PMID 7643402
  • Structure:1LDN_A; Geobacillus stearothermophilus allosteric LDH binds NAD; defined at 3.5A contacts.
    View structure with Cn3D
  • Citation:PMID 1731077

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             ###                    ##                                           ##  
1HYH_C      2 RKIGIIGLGNVGAAVAHGLIAQGVaDDYVFIDANeAKVKADQIDFQDAMANle--aHGNIVINDWAALADADVVISTL-- 77  Weissella confusa
AAV27786   27 IKIALIGAGNVGNSFLYAAMNQGLaSEYGIIDINpDFADGNAFDFEDASASlp--fPISVSRYEYKDLKDADFIVITA-- 102 Mycoplasma hyopneu...
Q98PG4      2 KKVVLIGTGNVGVTVVYTMITKGIdAEYVLIDINtEFAKGHAMDMSDAIALns-ttGSKIRTGTYADAKGADLLIVAA-- 78  Mycoplasma pulmonis
O52354      2 KKIAVIGCGFVGSTYILDLLQQGVqADYLLVDKNtNLADGHVRDLRDSKSLks-hnGSTFNVGTYDDLKDADVVAITAsi 80  Mycoplasma gallise...
AAZ43870    2 KKIIVIGMGNVGTTFVNIALARGLqANFVFVDKNeEICQAHVHDFQDMIALmprnnSTFKVGTLLQEAKDADIVIVTAs- 80  Mycoplasma synovia...
Q6KIP9      6 KRVAMVGAGLVGVSVLYSCMNRGLaEQYGIIDINdKLSVGHSLDFEDASAAnn--hNFSVGKIEYSDLKDYDVVVITA-- 81  Mycoplasma mobile
BAE03834    4 HKIVLIGSGYVGSAFAHAIVAKGLvDEMAIIDIDeDKAKADVWDLNHATPFgd--nFVNVHVGQYEDFKDADIVVICA-- 79  Staphylococcus hae...
Q9CGG8      6 KKVVIVGAGAVGSTYAHNLVVDDLaDEIAIINTNkSKASANSLDLLHALPYln-aaPKNIYAADYSDVSDADIVVLSA-- 82  Lactococcus lactis...
Q8CMZ0      6 KKVVLVGDGSVGSSYAFAMVTQGIaDEFVIIDIAkDKVEADVKDLNHGALYss--sPVTVKAGEYEDCKDADLVVITA-- 81  Staphylococcus epi...
YP_805918   5 GNIILIGDGAIGSSYAFNCLTTGVgQSLGIIDVNeKRVQGDVEDLSDALPYt---sQKNIYAASYEDCKYADIIVITA-- 79  Lactobacillus case...
Feature 1      ##                                            ###                      #       
1HYH_C     78 -GNIKL---QQDNPTgdrfAELKFTSSMVQSVGTNLKESGFHGVLVVISNPVDVITALFQHVTGFPAHKVIGTGTLLDTA 153 Weissella confusa
AAV27786  103 -GRPQK---PGETRL----ELVADNIRIIREIALKVKESGFSGISIIVANPVDIITRAYRDASGFSDQKVIGSGTVLDTA 174 Mycoplasma hyopneu...
Q98PG4     79 -GRPQK---QGETRL----EMIADNSKIMKDIALEIKKSGFNGFTIVISNPVDILATVFQKVTNFPKEKVMSSGTFLDTS 150 Mycoplasma pulmonis
O52354     81 pTVPTAdgeVFTDRL----QLMTANVKILNEIALELKRVGFKGLSIIPTNPCDVMAGVYQKVTGFDPHKIISTGCQLETM 156 Mycoplasma gallise...
AAZ43870   81 -IPANK---DFSDRL----KLAEANSKLMKSFGDQLKEAKFKGIVIVAANPCDVMASVFTYASNLPFKKVISTGTLLDSA 152 Mycoplasma synovia...
Q6KIP9     82 -GRPQK---PGETRL----EMVADNAKIMSNIAKNIKKSGFKGVSIVVANPVDVMTFIYQHETGFDKNRVISSGTSLDSA 153 Mycoplasma mobile
BAE03834   80 -SAKLA---KGETRL----KLLEDNVDIFVPMIQRIVDSGFDGYFVLPSNPVDIMSYVVKRVSNFPKNKIIGSGTSLDTA 151 Staphylococcus hae...
Q9CGG8     83 -NAPSAtfgKNPDRL----QLLENKVEMIRDITRKTMDAGFDGIFLVASNPVDVLAQVVAEVSGLPKHRVIGTGTLLETS 157 Lactococcus lactis...
Q8CMZ0     82 -GAPQK---PGETRL----QLVEKNTKIMKSIVTSVMDSGFDGFFLIAANPVDILTRYVKEVTGLPAERVIGSGTVLDSA 153 Staphylococcus epi...
YP_805918  80 -GIAQK---PGQTRL----QLLAINAKIMKEITHNIMASGFNGFILVASNPVDVLAELVLQESGLPRNQVLGSGTALDSA 151 Lactobacillus case...
Feature 1                             #                                                       
1HYH_C    154 RMQRAVGEAFDLDPRSVSGYNLGEHGNSQFVAWSTVRVMGQPIvtla----dagdIDLAAIEEEARKGGFTVLNGKGYTS 229 Weissella confusa
AAV27786  175 RLQFAIAKRAKVSPNSVQAYVMGEHGDSSFVAYSNIKIAGEYFcayskltgidssNYEKELEYPVSRRAYEIINRKRATF 254 Mycoplasma hyopneu...
Q98PG4    151 RFRKFLSEKTGVPTNSVHGFVIGEHGDKSVVVFSRMQIGFSRLddflks-kalteDDLKWISEKTYKEAYEIINRKRSTY 229 Mycoplasma pulmonis
O52354    157 RTRKMISEALGVNSDSVEGFVVGEHGSGAIVPWSVFRVGNVPMkqliae-gkikeEYVKDIFSRVVKEAFEIIKFKKATY 235 Mycoplasma gallise...
AAZ43870  153 RFKKFIAQKFNVNADSVQASILGEHGSSAIPLWSTVKIGDATLenllks-kklksNELNQILKQTIDEAFYIFSRKGNTQ 231 Mycoplasma synovia...
Q6KIP9    154 RLRFEISKKLKVHPKSVQAFVLGEHGDSSVSVYSAATVSGKSFneivke-rgiskKELEDMHTTVYKKAYEIINRKGSTY 232 Mycoplasma mobile
BAE03834  152 RFQFFLSREFDVAPNQVYAPIIGEHGDSQVAVWSHAQIAGEPVldllps-ntnleAFKTSISNRTTQIGYDIYVRKGTTN 230 Staphylococcus hae...
Q9CGG8    158 RMRQIVAEKLQINPKSIHGYVLAEHGKSSFAAWSNVTVGAIPLttwlkkypnpefPTFDEIDQEIREVGLDIFMQKGNTS 237 Lactococcus lactis...
Q8CMZ0    154 RFRYLISKELGVTSSSVHASIIGEHGDSELAVWSQANVGGISVydtlke-etgsdAKANEIYINTRDAAYDIIQAKGSTY 232 Staphylococcus epi...
YP_805918 152 RLRSEIGLRYNVDARIVHGYIMGEHGDSEFPVWDYTNIGGKPIldwip--kdrqdKDLPDISERVKTAAYGIIEKKGATF 229 Lactobacillus case...
Feature 1                                                                                     
1HYH_C    230 YGVATSAIRIAKAVMADAHAELVVSNRRDDm----gMYLSYPAIIGRDGVLa-ETTLDLTTDEQEKLLQSRDYIQQRFDE 304 Weissella confusa
AAV27786  255 YGIGAAIAKIVSNIIKDTKNIMIAGANLRGeygfhgVNIGVPVVLGANGIEk-IIEISLNDKEKEKFAKSVAIIDKIYQD 333 Mycoplasma hyopneu...
Q98PG4    230 FGIGASVAEMAESVLYNQRRIFPIGIYLDEskpgggIYISRPAILGENGWEe-VKNYDLSPAEQKAFDESAINLKKHWDD 308 Mycoplasma pulmonis
O52354    236 FGIAESMSLITRAYIYNLNTVLGVGVQLDDkyvasgIYFTVPAVVGKHGWKl-HSKLQLSQEEQAAFDKSALNIQKVTKD 314 Mycoplasma gallise...
AAZ43870  232 FGIGTSLYEITDAILNNKKQVMNIGVKLPAnyknagIYISIPVIVGKNGYEylSVKPNFNNNELKQFEASTSHMAKVHKD 311 Mycoplasma synovia...
Q6KIP9    233 FGIGSTVAELVEAILTDSHAIFGVGVYLTGqygvkdLYIGVPTVLGSKGVVe-VINFNLTKEEQEKFVSSATILKGNIQK 311 Mycoplasma mobile
BAE03834  231 FGISLSLVRIVEAILFNKNIIMNVSSYVEGeyglsdVYIGVPTVINRNGADr-IIELALSKEELSQLHHSADIILDYQRQ 309 Staphylococcus hae...
Q9CGG8    238 YGIAASLARLTRAIFRNESVILPVSAYLTGeygqfdLYTGSPAIIDRTGVRa-VLELELTQEEQEKFKASTVLLKENFDS 316 Lactococcus lactis...
Q8CMZ0    233 YGIALALLRISKALLNNENSILTVSSQLNGqygfndVYLGLPTLINQNGAVk-IYETPLNDNELQLLEKSVKTLEDTYDS 311 Staphylococcus epi...
YP_805918 230 YGIAASLTRLTSAFLNDDRAAFAMSVHLEGeyglsgVSIGVPVILGANGLEr-IIELDLNPEDHKRLADSAAILKENLKK 308 Lactobacillus case...

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