1V3V,1ZSV,2J3H,2J3J,2W4Q,2ZB4


Conserved Protein Domain Family
PGDH

?
cd05288: PGDH 
Click on image for an interactive view with Cn3D
Prostaglandin dehydrogenases
Prostaglandins and related eicosanoids are metabolized by the oxidation of the 15(S)-hydroxyl group of the NAD+-dependent (type I 15-PGDH) 15-prostaglandin dehydrogenase (15-PGDH) followed by reduction by NADPH/NADH-dependent (type II 15-PGDH) delta-13 15-prostaglandin reductase (13-PGR) to 15-keto-13,14,-dihydroprostaglandins. 13-PGR is a bifunctional enzyme, since it also has leukotriene B(4) 12-hydroxydehydrogenase activity. These 15-PGDH and related enzymes are members of the medium chain dehydrogenase/reductase family. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES.
Statistics
?
PSSM-Id: 176190
View PSSM: cd05288
Aligned: 173 rows
Threshold Bit Score: 208.875
Threshold Setting Gi: 242211005
Created: 1-Aug-2005
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:1V3V: Cavia porcellus leukotriene B(4) 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase binds NADP, contacts at 4A
    View structure with Cn3D
  • Structure:2W4Q: Human zinc-binding alcohol dehydrogenadase binds NADP, contacts at 4A
    View structure with Cn3D
  • Structure:2ZB4: Human 15-ketoprostaglandin delta-13-reductase binds NADP, contacts at 4A
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                     #                  
1V3V_B         7 KAKSWTLKKHFqg------------kpTQSDFELKTVelpp----lknGEVLLEALFLSVDPYMRIASKRlk-------- 62  domestic guinea...
EAK82792       3 TNHQVLFNKTQptg----------lpkPGETTKPGSAeldid-nvalnGGVLTRNIAVSLDPTMRNRMKGnngyfs---- 67  Ustilago maydis...
XP_001398781   3 PNKSLILTNIPtgl-----------ptPHKDLTPLPRpldittpppnpHSLLTKLLYASLDPYMRGRMRPahiksy---s 68  Aspergillus nig...
XP_001413487   5 SNKAIIFKKAPegf-----------pkPGEDLVIEDRplel---vapeGGVVAQVLYASFDPYQRGRLRKpevksy---f 67  Magnaporthe gri...
XP_001835369 389 TNGRAIFNSIPegf-----------pePGKTIVYDESqkidvdtvqlnGGFLIKVLYLSVDPYMRGRMRDpskksy---s 454 Coprinopsis cin...
XP_002471343 244 KNGRVLFNETPtgh-----------pvPGRTTVYDGSqtidpdmvplnGGFLVKTLVVSIEPLILRKMNLldnreclfiq 312 Postia placenta...
XP_002474777   5 TNARLIFNEIPsvavysppsessglpePGKTTIYDTSetidlenvplhGGALIKTLVLSVDPYLRGRMRDpsiesy---v 81  Postia placenta...
XP_002475147  83 KNGRVLFNETPtdh-----------pvPGRTTVYDGSqtidpdmvplnGGFLVKTLVVSIEPLILRKMNLly-------- 143 Postia placenta...
EEU37022       5 SNKSFIYKKIPdiy-----------lvPGEHIVVEDRpldle-sapleGGILVKVFYTSLDPYIRDRTRDpsvedf---i 69  Nectria haemato...
EEY16283       5 SNKSFIYKKVPqgf-----------pvPGQDLVIEDRpidle-naplhGGVLVEVLYTSFDPYMRGRMRDpkvksy---s 69  Verticillium al...
Feature 1                                                                                        
1V3V_B        63 ----eGAVMMGQQVARVVESKns--aFPAGSIVLAQs-GWTTHFISdg------kgLEKLLTewpd----klplSLALGt 125 domestic guinea...
EAK82792      68 apyvvGQPVTAIGIAEVVRSEvn--dFAPGDIVYAWstGIEDYTIHpaaa---aatFRKFDPaqlnn---glsiTTYLGa 139 Ustilago maydis...
XP_001398781  69 ppyplDSPITAYAVAQVVHSTha--sFKTGEIIYARl-PIQEYSILldga---nspVQKIPTplps----gielSQYVGi 138 Aspergillus nig...
XP_001413487  68 pamtiGQPLENTSLLRVLESKse--rFAKGDVLKHSlcPNQQYVVLsaeqa-qqqsIFKVEEsse------felAHHLGv 138 Magnaporthe gri...
XP_001835369 455 paftlGQPMNGHGVGVVVRSEss--sVKVGDHVYGMl-EFQNYIVRpnl-----egLRIVDNkhn------lplSYYIGv 520 Coprinopsis cin...
XP_002471343 313 lvplaFLPTIALSMGALSYGQmstekMRESEVLTPQkrHIPPSAASsgnriaashlAYCLQHaclwitdahprsISNFG- 391 Postia placenta...
XP_002474777  82 paftkGKPLDNFGVGVVLRSEdp--aIKPGDHLYGYf-PFQQYLLSqdls--ipsmTTKLPDdkl-------pwSVYLGv 149 Postia placenta...
XP_002475147 144 ---viGQPISNFGVGVVLRSEds--aFKPGDHVYSGdiLFQEYFVAtdt-----slFQVLENkqn------lpwSVYVGv 207 Postia placenta...
EEU37022      70 saldvDTPIVSAIVGRVIRSEse--kYQTGDLVMAFygDHVEYALIkgdkl-edasLWKISNphg------ldiTYFLGv 140 Nectria haemato...
EEY16283      70 ppfdlDQPIVSASVVKVLRSRha--rVRRSATSSPSfyGQNAHKIH----------NPHNLPa-----------HYFIGi 126 Verticillium al...
Feature 1          #   #                        ###                   ##   #                 #   
1V3V_B       126 IGMPGLTAYFGLLEVCgv-kgGETVLVSAAAGAVGSVVGQIAKlKGC-KVVGAAGSDEKIAYLKQ---IGFDAAFNYKtv 200 domestic guinea...
EAK82792     140 AGMAGMTAYMSLKEIIgdlkpGNKMFVSGAAGAVGQLVVQLCHqAGV-GVIASAGSDEKCEFVRS---LGAEESINYKte 215 Ustilago maydis...
XP_001398781 139 LGMTGLTAYAGLFEIGep-kkGETVFVSSAAGAVGSVVGQLAKrIGC-RVIGSAGGERKRKYVVEe--LGFDECFDYRve 214 Aspergillus nig...
XP_001413487 139 LGMPGLTAYASLFEIGkp-kkGETILVSSAAGAVGQVVGQLAKrEGL-TVIGSVGSDDKVEYCKE---LGFDHVFNYKke 213 Magnaporthe gri...
XP_001835369 521 CGMPGKTAYMAWKEYSqa-kkGETAFVTTGAGPVGSFVIQLAKlDGL-KVIGSAGSDEKVEFMKS---IGADVAFNYKtt 595 Coprinopsis cin...
XP_002471343 392 VGVVLRSEDSAIKPGDhv-ysGDILFQEYFVATDASLFQVLENkENLpWSVYVGSSKRQISPMGSwplAGQTAHHGWRe- 469 Postia placenta...
XP_002474777 150 CGMPGQTAYYGWKEHAkp-npGDVVFVSTGAGVVGSVVIQLAKaEGL-KVIASAGSDEKVAFMKE---LGADVAFNYKte 224 Postia placenta...
XP_002475147 208 CGMPGQTAHHGWREFAha-pkGKVAFVSSGAGPVEATVIQLAKqDGL-KVIASAGSDEKVEFVKS---LGADVAFNYKke 282 Postia placenta...
EEU37022     141 LGMAGTTAFQGLYNIGkp-kkGETIFVSAASGAVGQLVGQLAKaEGL-KVIGSVGSQAKIDFVVNe--LGFDDAFNYKte 216 Nectria haemato...
EEY16283     127 LGIPGTTAYEGLYEIGkp-vaGETIFVSSAAGAVGQLVGQLAKaEGL-KVIGSAGSQEKIDFIINe--LGFDGAFNYKtd 202 Verticillium al...
Feature 1                                ##                               ##### #                
1V3V_B       201 nsLEEALKKas----pdGYDCYFDNVGGe-----------fLNTVLSQMKDFGKIAICGAISVYNRmdqlp--pgpsPES 263 domestic guinea...
EAK82792     216 -dLRAKLKAfaa---dtGIHYYFDNVGGd-----------qLEAYIDNAARYGIIVACGYISGYNAekpedvvypknLFN 280 Ustilago maydis...
XP_001398781 215 -gAKEALGRlaggeggsGVDVYFENVGGe-----------qLDAVLEVMNNGGRIVACGMVSQYNLkpeer-yglknLYN 281 Aspergillus nig...
XP_001413487 214 -sPDAALTRla----pnGIDIYYENVGGe-----------hLEAALKHMNKYGRIPTCGMISEYNVkpedq-kgikgLMN 276 Magnaporthe gri...
XP_001835369 596 -nTLEVLQKe------gPIDVYWDNVGGe-----------tLDAALEAANINARFIECGMIAGYNTggqp----vknLMY 653 Coprinopsis cin...
XP_002471343 470 --FAHAPKGkv-----aFVSSGAGPVGAtviqlakqdglktLEAAINAAAVGARFIECGMVSGYNKetpy---pvknLVL 539 Postia placenta...
XP_002474777 225 -kTSDVLARe------gPINIYWDNVGGe-----------tLEAAIAAAADNAQFIECGYITSYNGedyh----iknLQL 282 Postia placenta...
XP_002475147 283 -qIADVLQRe------gPTDIYWDNVGGe-----------tLEAAINAAAIGGRFIECGMVSGYNKetpy---pvknLML 341 Postia placenta...
EEU37022     217 -eYGEALKRla----peGIDIFWDGIGGp-----------qLDAALLAMKPHGRIVSCGTMVMYHTtpedl-ygvknLWV 279 Nectria haemato...
EEY16283     203 -ePNEALARla----pnGIDIYWDNVGGa-----------qLEAALNAINKNGRIIGCGSIVDYNVkpedr-ygvknLFN 265 Verticillium al...
Feature 1                  ###                                             ## # #      
1V3V_B       264 IIYKQLRIEGFIVYRwqg--dvrEKALRDLMKWvleGKIQYHEHVTkgfENMPAAFIEMLnGANLGKAVV 331 domestic guinea pig
EAK82792     281 VITKELKYQGFIVSSyip--kwlGKFMDEVPKMladGTIKNREDVTfgvYGAEDAFLRLFtGDNSGKVAV 348 Ustilago maydis 521
XP_001398781 282 VVTKRLRMRGFIVGDkdigpkwiKERNEKVTAWlqeGSINAKEDITvgiENGPEAFVAMLrGENLGKAVL 351 Aspergillus niger CBS 513.88
XP_001413487 277 IVSKEITMRGFLYQSiag--pwaAKFKEDVTKGlkdGSIKAKLHVVegiENGPEGFVGMLrGENFGKAVL 344 Magnaporthe grisea 70-15
XP_001835369 654 VVGKSISMHGFIVSRleh--kyeDKFYEEVPALvakGDLKYREQVFdglQSVGDAILAVQkGTNKAKAVI 721 Coprinopsis cinerea okaya...
XP_002471343 540 IVVKELQILGFLSSSlqt--khlANFYREIPPAvarGEIKYFEDRRlglENVGETILDMLtGQIKGKSVI 607 Postia placenta Mad-698-R
XP_002474777 283 IFAKQLTLSGFLINRlrh--kyqDAFYSEMPARvarGEIKYTEDITrglEKAGEAIVDVQtGKNKGKSVI 350 Postia placenta Mad-698-R
XP_002475147 342 IVVKELQILGFLSSSlqt--khlANFYREIPPAvarGEIKYFEDRRlglENVGETILDMLtGQIKGKSVI 409 Postia placenta Mad-698-R
EEU37022     280 LTNKYLKMEGFIVDLaie---kyEAMMEKVAPLiasGQFKVLQDITvgiENAPEALAGVFtGKNFGKAVL 346 Nectria haematococca mpVI...
EEY16283     266 IIGKSLTFKGFIVSLtpe---ryQAFNDKVQPLladGKVKAKVDITegiENAAEGLVGIFhGKNFGKAVL 332 Verticillium albo-atrum V...

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap