1PS0,1UUF,1YQX,2CF5


Conserved Protein Domain Family
CAD1

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cd05283: CAD1 
Click on image for an interactive view with Cn3D
Cinnamyl alcohol dehydrogenases (CAD)
Cinnamyl alcohol dehydrogenases (CAD), members of the medium chain dehydrogenase/reductase family, reduce cinnamaldehydes to cinnamyl alcohols in the last step of monolignal metabolism in plant cells walls. CAD binds 2 zinc ions and is NADPH- dependent. CAD family members are also found in non-plant species, e.g. in yeast where they have an aldehyde reductase activity. The medium chain dehydrogenases/reductase (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH), quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. Active site zinc has a catalytic role, while structural zinc aids in stability. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines.
Statistics
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PSSM-Id: 176186
View PSSM: cd05283
Aligned: 112 rows
Threshold Bit Score: 305.189
Threshold Setting Gi: 195999816
Created: 27-Jul-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:putative NAD(P) binding site [chemical binding site]
Evidence:
  • Comment:MDR family binds NAD(P) as a cofactor
  • Comment:defined by comparison to MDR alcohol dehydrogenase like family

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                     ###  #                             
1PS0_A         8 EGIAIQShed--wknPKKTKYDPKpfy-----dhDIDIKIEACGVCGSDIHCaaghwgnmkMPLVVGHEIVGKVVKLGPK 80  baker's yeast
1YQX_B        18 FGWAARDqs----ghLSPFNFSRRatg-----eeDVRFKVLYCGVCHSDLHSikndwgfsmYPLVPGHEIVGEVTEVGSK 88  quaking aspen
XP_383217     11 TVYRSGDgn----ggFTPTTVKVTtlg-----phDILVRITHSGVCHTDITFcq-----mgAPIALGHEGVGIVEAVGSL 76  Gibberella zeae...
XP_001246363   9 HEFAVFKgtk---sgKVVRNITRRaal----qgnEVLVRVTHSGLCGSDLHYr-------nSGCVLGHEGVGIVSEVGPN 74  Coccidioides im...
XP_001398603   6 KVFCGSSdg----kvVEKLTTRRLgd-------nDVFIETTHSGLCGTDEHFl-------hCDQALGHEGVGVVKHVGPS 67  Aspergillus nig...
EDP55523      11 KGSESGD--------VIPDTIRRTiq------psEVFVEISHAGLCGTDRLFk-------hKGIALGHEGAGTVRDVGSA 69  Aspergillus fum...
XP_002109776  11 TSYQLRDteay-lfnIEKIEVPPLqp-------sDCFVKIECCSVGKLDLLVr--------EGQVPGHEIVGSVVAVGSD 74  Trichoplax adha...
XP_002116488   7 EAYVVDD--------VSKQTFRKAqidvpqlqshDVLVNIECCSVCHTDFFCp--------AGKAPGHEFIGKIVAVGSN 70  Trichoplax adha...
CAP92984      13 EGKIVAD--------KTTRTLQPN----------EVYIETTHSGLCGTDEHYl-------sSGMALGHEGVGIVRQVGRD 67  Penicillium chr...
EEQ35822       6 EGYEFTTfrgtdagvVASQTKRPPlt------rnQVLVSITHSGVCGTDQHYr-------kAGCVLGHEGIGIVQCLGPD 72  Microsporum can...
Feature 1                                                                                        
1PS0_A        81 SNsgLKVGQRVGVGAqv-fSCLECDRCkn--dnEPYCTKfvttysqpyedgyvSQGGYANYVRVhehFVVPIPEnIPSHL 157 baker's yeast
1YQX_B        89 VKk-VNVGDKVGVGClv-gACHSCESCan--dlENYCPKmiltyasiyhdgtiTYGGYSNHMVAnerYIIRFPDnMPLDG 164 quaking aspen
XP_383217     77 VTq-FNVGDRAGGGFhr-dACGHCKYCls--gkDIYCYNrvifg-----egdfDNGTFGKYYIGketYLHKIPEgLASEH 147 Gibberella zeae...
XP_001246363  75 VLk-LREGDRVGWGYlq-nSCAHCKQCin--ghQIHCPDrqtfg-----nhnkDQGSMGHAAIWtepFLFRIPEsITSAD 145 Coccidioides im...
XP_001398603  68 VSs-VKVGDRVGFGFir-rVCGRCDNCis--gcDHHCREkrayg-----qhdfDVGSFSHGTVWdadAVYPIPEgYDSAH 138 Aspergillus nig...
EDP55523      70 VTt-FKVGDKVGFGWvr-kVCGHCDFCvs--ghDQYCQQreqyg-----kgntEIGAFATHAVWhesMLVKLPDdLEPEY 140 Aspergillus fum...
XP_002109776  75 VEg-IKPGMRVGMGFfnddYYTKFEQPtstlsyEDSTSDtsan-------skiAYGGFADGVVWdarYVYPIPKtMSSIA 146 Trichoplax adha...
XP_002116488  71 VVk-LKVGQRVGGGWql-gSCQNCNVCnd--gkQHICPEktnf-------inaPIGGFAEYVVWdsrFLYDIPEtMSSAD 139 Trichoplax adha...
CAP92984      68 VTn-VKVGARVGYGYth-yVCGNCDKClt--gwDQYCENkkeyg-----shdhDLGSFSGGTVWdagCVVPIPDgYDSAD 138 Penicillium chr...
EEQ35822      73 VRa-LRIGQRVGWGYv---HGSYDQFCss---rKVYGVTn------------lDQGSMAYAAIWpesSLYAIPEkLSSLD 133 Microsporum can...
Feature 1             #   #                    ######                 ##   #               #     
1PS0_A       158 AAPLLCGGLTVYSPLVRNgc-gPGKKVGIVGLGGIGSMGTLISKAMGAETYVISRSsRKREDAMKMG-ADHYIATleegd 235 baker's yeast
1YQX_B       165 GAPLLCAGITVYSPLKYFgldePGKHIGIVGLGGLGHVAVKFAKAFGSKVTVISTSpSKKEEALKNFgADSFLVSrdqeq 244 quaking aspen
XP_383217    148 AAPLQCAGATVYAALKATv--tPEKRVGILGIGGLGHLAIQYANKMGADVIIYSTStSKETEARQLG-AKEFHLLdnmfd 224 Gibberella zeae...
XP_001246363 146 AAPLMCAGATVFSIFDQYni-rPTDRVGILGLGGLGHIAVQFSKHMGCQTVVFSGTnDKKNEALSLG-ADEFYPTrgvee 223 Coccidioides im...
XP_001398603 139 AAPLLCAGASVWACLTNNgi-rPSDRVGVMGIGGLGHLAIKLARALRYNVVALSSSeKKREEALEFG-ASEFYRFpntqt 216 Aspergillus nig...
EDP55523     141 AAPLMCGGATVWCALTCYdi-kPGERVGIQGIGGLGHMAIQFASKLGCDVVVFSSSaAKKDEAKGLG-ANEFHTLengav 218 Aspergillus fum...
XP_002109776 147 AAPILFTGSIAYSALCDAgi-qPGNRVGVLGIGGIGHLAVQYARAWGCKVIALSTKlEKSQDALKFG-AQEFYDVgdeel 224 Trichoplax adha...
XP_002116488 140 AAPLLCAGITVYAPLANHnv-qPGSRVGILGVGGLGHLGIKFAKAWGCRVVALSTSsNKEKEARAFG-ADEFVVTkdadq 217 Trichoplax adha...
CAP92984     139 AAPLMCAGATVWTCLTEYgv-kPTDRVAIMGIGGLGHLAIKLASAMGCHVVVLSSSeTKREEAFGFG-ASEYHVFrkgee 216 Penicillium chr...
EEQ35822     134 AAPMMCAGASVFGIYEQCgi-rPNDRVGIIGLGGLGHMAVIFGAKLGVETVVFTHSvAKQVDAIVMG-ATEFIPLedlpq 211 Microsporum can...
Feature 1                                                    ## #                              ##
1PS0_A       236 w------------------------------gekyfdTFDLIVVCASSlt------diDFNIMPKAMk---vGGRIVSIS 276 baker's yeast
1YQX_B       245 m-------------------------------qaaagTLDGIIDTVSAv--------hPLLPLFGLLk---sHGKLILVG 282 quaking aspen
XP_383217    225 ---------------------------------dvkaPIDVLVICGTKy--------pDWDRVMAKEfl-arDGVIVPLA 262 Gibberella zeae...
XP_001246363 224 --------------------------------fkdiePVDHLIVTTSAq--------vDWALYLPVLa---pRSSVYPLT 260 Coccidioides im...
XP_001398603 217 pnhik-----------------------pvkhlllcgSSDVDYASWVShlpskqnaidHANIYRSLLdlvdtNGTIYHIS 273 Aspergillus nig...
EDP55523     219 --------------------------------gdavrPVKHLLWCGNEp--------pDFSKLVPPSl---sAGDLSMQQ 255 Aspergillus fum...
XP_002109776 225 --------------------------------ltrapKMDCILITSPVal-------kDYDFFISLLq---pGGTLAILG 262 Trichoplax adha...
XP_002116488 218 --------------------------------lkslkPLDMIINTVSAn--------lDYESYINLLa---pRGKFVVIG 254 Trichoplax adha...
CAP92984     217 --------------------------------mkdfkPVNHLLLCGSAn--------vDYPSLIPLMd---vHGSIYPLT 253 Penicillium chr...
EEQ35822     212 iisarrkrrnsdsgiddqerrssmdddtsedetrgirPLDHLIVTTTEd--------vVWSTFFELMa---pRGTVHLLA 280 Microsporum can...
Feature 1                                  ###                                                   
1PS0_A       277 IPeqhe--mlsLKPYgl--kAVSISYSALGSIKELNQLLKLVSEKDIKIWVETLPVg-eAGVHEAFERMEKGdvRYRFTL 351 baker's yeast
1YQX_B       283 APekp----leLPAFsliagRKIVAGSGIGGMKETQEMIDFAAKHNITADIEVIST---DYLNTAMERLAKNdvRYRFVI 355 quaking aspen
XP_383217    263 APvhgplslpaGAMFf---qGYHVFSSLVGSRNVHDEMLEFSARHGIKPMVQIFKHegaETIREVFELVQMNkmRYRAVL 339 Gibberella zeae...
XP_001246363 261 VAfgdf-tipyMPLLr---qGLKIVGSIVASVPVHQRMIEFAARHNIKPTVELFDMd-eEGIERAMDRLAEGkiRYRAVL 335 Coccidioides im...
XP_001398603 274 VTlkp----tpIPLVpfgqkGIRIQGCFITSRRNLQELLEFAARFDIKPTIMTFPLt-rNGLEETIEKLRAGriRYRAVL 348 Aspergillus nig...
EDP55523     256 PPlp------vMPLIs---nGIRVQGSAVASRVSVRKMLRFVSVHRIRPIIMTWPMt-kDGIQAAFQILEQGkmRYRGVI 325 Aspergillus fum...
XP_002109776 263 HPdkpi-iinsSYLIs---eSKRVIGVAVSSPLTVMKALEFAAEKNIVSQTELLEMn-eINCTVALQRVKSSsvRYKTVL 337 Trichoplax adha...
XP_002116488 255 IAskpl--qvnGGLLig--sERSIVGSLVGSPIMMQKMLNFAGMHNISPQLEIYPMd-vDHCHTAFERIDQNlaRYRIVL 329 Trichoplax adha...
CAP92984     254 VDfsh----spVPLLfmnlkGIRIQGSLVASRNSLRTLVKFAADKKITPTTMTFPLt-kDGVESAMQTLRDGkmRYRGVL 328 Penicillium chr...
EEQ35822     281 VSlkddltvpsAALId---sGLRVLGSLVAPRSVMIRMLDFAARNRVKPIVQKFPLt-aDGVNTAMKKLDEGsiRYRGVL 356 Microsporum can...
Feature 1         
1PS0_A       352 V 352 baker's yeast
1YQX_B       356 D 356 quaking aspen
XP_383217    340 E 340 Gibberella zeae PH-1
XP_001246363 336 V 336 Coccidioides immitis RS
XP_001398603 349 E 349 Aspergillus niger CBS 513.88
EDP55523     326 V 326 Aspergillus fumigatus A1163
XP_002109776 338 Y 338 Trichoplax adhaerens
XP_002116488 330 T 330 Trichoplax adhaerens
CAP92984     329 V 329 Penicillium chrysogenum Wisconsin 54-1255
EEQ35822     357 E 357 Microsporum canis CBS 113480

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