1GU7,1GYR,1N9G,1N9G,1N9G,3GMS,3IUP


Conserved Protein Domain Family
ETR_like

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cd05282: ETR_like 
Click on image for an interactive view with Cn3D
2-enoyl thioester reductase-like
2-enoyl thioester reductase (ETR) catalyzes the NADPH-dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the alcohol dehydrogenases in this family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes, or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding. Candida tropicalis enoyl thioester reductase (Etr1p) catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis. Etr1p forms homodimers with each subunit containing a nucleotide-binding Rossmann fold domain and a catalytic domain.
Statistics
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PSSM-Id: 176645
View PSSM: cd05282
Aligned: 40 rows
Threshold Bit Score: 224.079
Threshold Setting Gi: 258588716
Created: 26-Jul-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NADP bindingdimer interface
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:NADP binding site [chemical binding site]
Evidence:
  • Structure:1N9G: Candida tropicalis mitochondrial 2-enoyl thioester reductase binds NADP, contacts at 4A
    View structure with Cn3D
  • Structure:3IUP: Ralstonia eutropha putative NADPH:quinone oxidoreductase binds NADP, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                  #                                     
1GU7_A         7 VLYTQHGEPk--dVLFTQSFEIdddnlapnEVIVKTLGSPVNPSDINQi--qGVYPSKPakttgfgttepaaPCGNEGLF 82  Candida tropicalis
1N9G_A        29 VLYTQHGEPk--dVLFTQSFEIdddnlapnEVIVKTLGSPINPSDINQi--qGVYPSKPakttgfgtaepaaPCGNEGLF 104 Candida tropicalis
NP_384990      4 TLVRQFGDPg--qVIELVDAPRpap--gtgEVEVEISLAAINPSDLIPv--tGAYSARTal---------pfVPGFEGVG 68  Sinorhizobium m...
NP_801206      9 VNQFCFGQPktslSLEKVPLKPla----qgKVRVRLEATNINPSDLLSihgvGQYRRAHvp---------prVPGFEAVG 75  Vibrio parahaem...
YP_204361      3 AIIQQFGPAlesvQLEHYSPSEpl----inELQVEMKLSTINPSDLITi--sGAYRSRItl---------pfVPGFEGIG 67  Vibrio fischeri...
YP_764876      9 AVVRKFGPAq---EVVELERAAlppl-rrdQVRVRLLARAINPSDIITi--sGAYSGRTtl---------pfIPGFEAFG 73  Rhizobium legum...
YP_001176636   6 LGYRAFGEPesvlQIELRSPEPlp----agQIRVHMLFSPVNASDLIPm--tGAYRHRTpl---------paVAGYEGVG 70  Enterobacter sp...
YP_001906765   9 AMVREFGNIedilRLEQAELPEla----agQVRVNMSFATINPSDLITi--sGAYRSRIal---------pfVPGFEGVG 73  Erwinia tasmani...
YP_002238829  17 IVYDRYGPPaavlTLKRLPLAGg-------RVRVRMRYAPVNPSDLIPv--tGAYRHRTrl---------paVAGYEGLG 78  Klebsiella pneu...
YP_002263311   3 AIVHQFGLAp--qSITVEPYTSepl--tpfQIQLKMRYSTINPSDLITi--sGAYRSRIpl---------pfIPGFEGLG 67  Vibrio salmonic...
Feature 1                                                                           ##  #        
1GU7_A        83 EVIKVGSNVss-leAGDWVIPShvnfGTWRTHALGndDDFIKLPNpaqskangkpngltinqGATISVNPLTAYLMLTHY 161 Candida tropicalis
1N9G_A       105 EVIKVGSNVss-leAGDWVIPShvnfGTWRTHALGndDDFIKLPNpaqskangkpngltinqGATISVNPLTAYLMLTHY 183 Candida tropicalis
NP_384990     69 IVRRVGPDVqg-fkAGDRVVPIga-sGLWQQFLLRpaEWCFHVPGgie-----------daqAAMSYVNPLTALTLAEAL 135 Sinorhizobium m...
NP_801206     76 RVVEVSAVGqtgfqVGQKVLVAm--sGTWQYYIDAspENLFPLPEsld-----------ngyACQLYINALTAWVITTKV 142 Vibrio parahaem...
YP_204361     68 KVTKCSDSTsi-fsIGDRVLPIgt-aGAWQKYRNTkeEWCFTIPEnls-----------deqAATSYINPMTAWLMLTEA 134 Vibrio fischeri...
YP_764876     74 VVEQCGEEVhg-lsPGTRVLPVrs-aGGWQEFKDTdpGWCLRVPDalt-----------dfeAATSYVNPMTAWLMLHAK 140 Rhizobium legum...
YP_001176636  71 VVTDGPQHL-----LGKRVLPLrg-qGTWQNIVDCpaALAIPVPDdid-----------stlAARAYINPLAAQMMLNLY 133 Enterobacter sp...
YP_001906765  74 SICQSNDPAl---aVGQRVLPVgs-mGAWQNYKDSeaQWCFTLPDfvs-----------drqAATGYVNPMTALLMLTEE 138 Erwinia tasmani...
YP_002238829  79 EVVAAPYGSrl--sAGQRVLPLrg-gGTWQRFIDLdeTWLVPVPPavd-----------dllAARGYINPLTAMLMLKRW 144 Klebsiella pneu...
YP_002263311  68 IIKERYDSHsa-fsIGDRVLPIgs-aGAWQRYRNIdeKWCFTIPNqls-----------deqAATSYINPMTAWLIVSER 134 Vibrio salmonic...
Feature 1                       # ####                   #                                       
1GU7_A       162 vkl--tpgKDWFIQNGGTSAVGKYASQIGKLLNFNSISVIRDrpnldeVVASLKEL-GATQVITEDqnnsreFGPTIKEW 238 Candida tropicalis
1N9G_A       184 vkl--tpgKDWFIQNGGTSAVGKYASQIGKLLNFNSISVIRDrpnldeVVASLKEL-GATQVITEDqnnskeFGPTIKEW 260 Candida tropicalis
NP_384990    136 rehfgsleGMDVGVTAAGSAIGGMLMKLLALEGAVPTAMLRSd----rSRGRLGET-HRILVADSGd----gLMGSQFDA 206 Sinorhizobium m...
NP_801206    143 akl---nkDDVVIINAAGSAIGKIFAQLAHSLGFTLIAVTSKp-----DEYPYDTIpVLDAKQDLH------AQLQTRKL 208 Vibrio parahaem...
YP_204361    135 lni---hsDMSIIVNAANSAIGLMLIRMLNHLGITPIALVRRd----nTIEEFENC-RVHTIINTSnn---aDYQQLLDI 203 Vibrio fischeri...
YP_764876    141 igl---rpGMRIAINAAASSIGAILIGLANAAGVEPVAIVRSe----gSLERLRGR-VEAIIIDREes---dLVAGLAGR 209 Rhizobium legum...
YP_001176636 134 sp-----qGKWILLTAAGSDCAALLGQWAINAGADAVYGIQRsp---aHGERLRAM-GIIPVSQQDta---aISAIAARA 201 Enterobacter sp...
YP_001906765 139 lnf---tpGMRIMINAANSAIGKMLIRIANHRGLEPIAIVRKa----eNLSLFSGY-ATRQLLDSSap---dYPQALAAL 207 Erwinia tasmani...
YP_002238829 145 pv-----aGKHLVLTAASSSCASLLGQWALAMGARSVSGIIRsp---qHRARLEQA-GIYPILDTDr----aLMEKVSQH 211 Klebsiella pneu...
YP_002263311 135 lhr---hkEMTLVINAANSAIGLILIRMLNHLGITPIALVRRd----nTEADFEGC-NVRRIINIQnk--nsIQQLLESK 204 Vibrio salmonic...
Feature 1                     ##                     ## #                                 ###    
1GU7_A       239 ikqsggeaklALNCVGGKSSTGIARKLn-NNGLMLTYGGMSfq------pVTIPTSLYif------knFTSAGFWVtell 305 Candida tropicalis
1N9G_A       261 ikqsggeaklALNCVGGKSSTGIARKLn-NNGLMLTYGGMSfq------pVTIPTSLYif------knFTSAGFWVtell 327 Candida tropicalis
NP_384990    207 ----------VLDAVGGTLAGELIGRSirPGGTFIQYGALSga------pVPQTAISGrp-------dIRFAFLWLrtwv 263 Sinorhizobium m...
NP_801206    209 pqp-----tvALDAIGGEASTDLIRTLk-ENGQYINYGTLSlap----ytPVFFESVKan-------nIDFSTFFLrywe 271 Vibrio parahaem...
YP_204361    204 tknn--kidaVLDFIGGDDALLYTHIIk-EHGQFINYGLLSkqpipadfwIQRPDIQFs--------yFHLRQWIHsaek 272 Vibrio fischeri...
YP_764876    210 hgv-----daVLDCVGGARAATLADALk-PGGHFVHYGLLSg--------QSIPASFWashpdiafsyCHLREWVHsqam 275 Rhizobium legum...
YP_001176636 202 e--------iVYDATGGRLADRILDAMp-ETGLFVCYGLLSg--------QPFRQQSQvr-------wFHIRNYLDalsv 257 Enterobacter sp...
YP_001906765 208 qrsg--gvaaIFDCIGGEESLTLAQALt-PGGQFIHYGLLSg--------QPIPPAFWrsr-----pdIRFSHFHLrmwv 271 Erwinia tasmani...
YP_002238829 212 sd-------lVFDAVGGELANTLLSVLp-ASSTLISYGLLSg--------RPLTQTRGs---------ATVRKFHLreal 266 Klebsiella pneu...
YP_002263311 205 qstg---idaVLDCVGGIEALQLSHLLk-EGGQFINYGLLSg--------KPIPPTFWrer-----pdIDFSYFHLrqwi 267 Vibrio salmonic...
Feature 1                                                                             
1GU7_A       306 kn-----nkelkTSTLNQIIAWYEegkltdaksietlydgtkplheLYQDGVANs------KDGKQLIT 363 Candida tropicalis
1N9G_A       328 kn-----nkelkTSTLNQIIAWYEegkltdaksietlydgtkplheLYQDGVANs------KDGKQLIT 385 Candida tropicalis
NP_384990    264 hsagrealeaafLRSFAGLQSGLFaspiaa-----------typlsRLADALAHqad--prRDGKILLD 319 Sinorhizobium meliloti 1021
NP_801206    272 es----vgkggrKTVFAEMLKHFIandiklav-------ahylpleAFQTAIEQieekslaRSGKIILT 329 Vibrio parahaemolyticus RI...
YP_204361    273 ---------pliQHKLNEVMNLVHqgiadtti-------dryfslkEIHHAIQYiektrtvNKGKVLIT 325 Vibrio fischeri ES114
YP_764876    276 ---------gdvQQAYSEIAAQIVskviatev-------revfpleNVRQALHSalp--frAGGKVLLA 326 Rhizobium leguminosarum bv...
YP_001176636 258 ---------dqwQAEFCEIWPKLQascygdv---------tlfplaEWRSALANyrq--agRLAKPMFA 306 Enterobacter sp. 638
YP_001906765 272 hh----hdkalvQGKIDEVMALIRdgviatei-------vaeyalaEIDKAVAAvrs--geLKGKILLR 327 Erwinia tasmaniensis Et1/99
YP_002238829 267 pt----lsvaawRAAFDEIWQRLPvttqppa---------qrialnDWREAIAAadq--pgRGGKILLD 320 Klebsiella pneumoniae 342
YP_002263311 268 hs----sekniiQEKLNEVMLLVYsgiadtii-------sahfslnDIHKAITYiqdkkktKKGKILIT 325 Vibrio salmonicida LFI1238

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