3UHA,2QRK,2QRJ,2QRL


Conserved Protein Domain Family
SDH_like
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cd05199: SDH_like 
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Saccharopine Dehydrogenase like proteins
Saccharopine Dehydrogenase (SDH) and related proteins, including bifunctional lysine ketoglutarate reductase/SDH enzymes and N(5)-(carboxyethyl)ornithine synthases. SDH catalyzes the final step in the reversible NAD-dependent oxidative deamination of saccharopine to alpha-ketoglutarate and lysine, in the alpha-aminoadipate pathway of L-lysine biosynthesis. SDH is structurally related to formate dehydrogenase and similar enzymes, having a 2-domain structure in which a Rossmann-fold NAD(P)-binding domain is inserted within the linear sequence of a catalytic domain of related structure. Bifunctional lysine ketoglutarate reductase/SDH protein is a pair of enzymes linked on a single polypeptide chain that catalyze the initial, consecutive steps of lysine degradation. These proteins are related to the 2-domain saccharopine dehydrogenases.
Links
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Statistics
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PSSM-Id: 240623
Aligned: 24 rows
Threshold Bit Score: 358.084
Created: 18-Jul-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:3UHA: Saccharomyces Cervisiae Saccharopine Dehydrogenase bound to NAD; contacts at 4A
  • Structure:2QRK: Saccharomyces cerevisiae Saccharopine Dehydrogenase bound to AMP; contacts at 4A
  • Comment:Saccharomyces Cervisiae Saccharopine Dehydrogenase retains the 3D arangment of the GxGxxG(17-18x)D NAD binding site motif, but has altered spacing between the conserved residues: GxxGxxG(17-20x)D
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                      
3UHA_A       5 TLHLRAETKp-LEARAALTPTTVKKLIAk------GFKIYVEDSPQSTFNINEYRQAGAIIvpagswkTAPRDRIIIGLK 77   Saccharomyces ce...
2QRK_A      26 TLHLRAETKp-LEARAALTPTTVKKLIAk------GFKIYVEDSPQSTFNINEYRQAGAIIvpagswkTAPRDRIIIGLK 98   baker's yeast
2QRJ_A      26 TLHLRAETKp-LEARAALTPTTVKKLIAk------GFKIYVEDSPQSTFNINEYRQAGAIIvpagswkTAPRDRIIIGLK 98   baker's yeast
NP_567914   16 VVGILAETVnkWERRTPLTPSHCARLLHggkdrtgISRIVVQPSAKRIHHDALYEDVGCEIs-----dDLSDCGLILGIK 90   thale cress
AEA45585     5 KLGIIREGKtpPDKRVPLTPKQCKLVEMkf----pQVKVFVQESEVRAFKDEEYSAEGIEVv-----kDLSDCDIIMGVK 75   Fluviicola taffe...
EEG43351     3 SFGIIKERKnpPDKRVVLTPEFCQVIKDey----sNAQIIVEPSDIRVFKDHEYQEKGLLVs-----aSMEDCEVLLGVK 73   Flavobacteria ba...
AEV32513     2 KFGIIREGKtpPDKRVPLTPQQCKALKAky----pEMEILVQPSDVRAFKDKEYEAEGSTIa-----eDLSDCEVLLGVK 72   Owenweeksia hong...
ADF53857     3 KFAIIREEKtpPDRRVVFSPKHLKQAKNqf----pEAHFKVQQSDIRIFRDEEYQNEGFQVs-----qDISDCDVMIGVK 73   Zunongwangia pro...
EHQ44518     2 KIGIIKERKspPDRRVVFTPEILAKALQtf----dTLEAVVEPSPIRIFPDQAYADKNIPLs-----dDLSDCDVLIGVK 72   Myroides odoratu...
AEE12903     7 TIGLPKETKtpVDGRVMFSPDQAAEIMLr-----yPIEIIVESSAVRCFPDRQYQDMGIQVvd---nlTSVDCDIIIGIK 78   Porphyromonas as...

Feature 1                                                                                      
3UHA_A      78 EMPEtDTFPLVHEHIQFAHCYKdqAGWQNVLMRFIkgHGTLYDLEFLENDqGRRVAAFGFYAGFAGAALGVRDWAFKqth 157  Saccharomyces ce...
2QRK_A      99 EMPEtDTFPLVHEHIQFAHCYKdqAGWQNVLMRFIkgHGTLYDLEFLENDqGRRVAAFGFYAGFAGAALGVRDWAFKqth 178  baker's yeast
2QRJ_A      99 EMPEtDTFPLVHEHIQFAHCYKdqAGWQNVLMRFIkgHGTLYDLEFLENDqGRRVAAFGFYAGFAGAALGVRDWAFKqth 178  baker's yeast
NP_567914   91 QPEL-EMILPERAYAFFSHTHKaqKENMPLLDKILseRVTLCDYELIVGDhGKRLLAFGKYAGRAGLVDFLHGLGQRyls 169  thale cress
AEA45585    76 EVNI-SDLIPQKKFLFFSHTIKkqPYNRNLLRAILekKIQLIDYEVLKSKeNKRIIGFGRYAGIVGAYNGIRAYGEKtgs 154  Fluviicola taffe...
EEG43351    74 EVPI-EALIANKKYFFFSHTIKkqAYNRDLLRAVLekNISLFDHEVITSArGQRLVAFGRYAGLVGAYNGLRAYGLKhel 152  Flavobacteria ba...
AEV32513    73 EVPL-DMLIPNKTYMFFSHTYKkqPYNSKLLKTILekKIRLIDYEMLTNEfGIRLLGFGRFAGIVGAYNAFRAWGEMthd 151  Owenweeksia hong...
ADF53857    74 EVPI-KSLIPNKKYFFFSHTIKkqPYNRDLLKAILdkNIELFDHEVITSEsGMRLIGFGRYAGLVGAYNGFRAIGLKnef 152  Zunongwangia pro...
EHQ44518    73 EVPV-EDLIPNKTYFFFSHTIKeqEYNRGILQTCLakNIRLIDHETLVDEnNKRLIGFGRYAGIVGAYNTLRAFGLKyel 151  Myroides odoratu...
AEE12903    79 EQEL-THIIPNRHYVFFGHFAKeqEYNRSLAKHLLsnKNTFTDHEYLVDNsNKRLCAFGRYAGIVGAYNAIWGYGLKekq 157  Porphyromonas as...

Feature 1                                                        #  ##                      ###
3UHA_A     158 sddedlpavspypneka--------lvkdvtkdykealatgarkPTVLIIGAlGRSGSGAIDLLhKVGIpdaNILKWDIK 229  Saccharomyces ce...
2QRK_A     179 sddedlpavspypneka--------lvkdvtkdykealatgarkPTVLIIGAlGRCGSGAIDLLhKVGIpdaNILKWDIK 250  baker's yeast
2QRJ_A     179 sddedlpavspypneka--------lvkdvtkdykealatgarkPTVLIIGAlGRCGSGAIDLLhKVGIpdaNILKWDIK 250  baker's yeast
NP_567914  170 lgystpflslgasymysslaaakaavisvgeeiasqglplgicpLVFVFTGT-GNVSLGAQEIFkLLPH---TFVEPSKL 245  thale cress
AEA45585   155 fqlkpahectgr-----------------kemeeelqhvdfpsdMKLVLTGF-GRVGYGAREIMdLLPF---TEVSPDEF 213  Fluviicola taffe...
EEG43351   153 felpkasdlrdl------------------ealiaaiksvtlppIKVVLTGV-GRVGSGAAELLdAAGV---LKVCPDHF 210  Flavobacteria ba...
AEV32513   152 yslkpahecadr-----------------remeselikvklpskAKIAITGG-GRVASGAQEIFsAMHL---KQVTPKKY 210  Owenweeksia hong...
ADF53857   153 yslpkaddlpdl------------------namlaqldkikvpnLKIVLTGS-GKVARGAKEILdHLKI---KQVEVPDY 210  Zunongwangia pro...
EHQ44518   152 ftlkkaeqmlht------------------edliaqlkkqyfppIKIAVTGN-GKVSHGIMEILnGMKA---KKVSIEHF 209  Myroides odoratu...
AEE12903   158 yalsspnslgvv-----------------eklleavklinieklPNILFTGN-GNVAKGCREFLtAIGY---EETTIEDY 216  Porphyromonas as...

Feature 1                                                                              ###     
3UHA_A     230 ETsrggp-----------------------------------------------------fdeipQADIFINCIYLskpi 256  Saccharomyces ce...
2QRK_A     251 ETsrggp-----------------------------------------------------fdeipQADIFINCIYLskpi 277  baker's yeast
2QRJ_A     251 ETsrggp-----------------------------------------------------fdeipQADIFINCIYLskpi 277  baker's yeast
NP_567914  246 PElfvkdkgisqngistkrvyqvygciitsqdmvehkdpsksfdkadyyahpehynpvfhekispYTSVLVNCMYWekrf 325  thale cress
AEA45585   214 LAekfetpvfthleiedyyrrkdgk-----------------pfskaefystpeiyessftryseAADIYIACHFWsnks 276  Fluviicola taffe...
EEG43351   211 MGrhftgpvytqidvleynkrsdgl-----------------vgdkkdffdnpeayvsnflpyteEADVFIAGHFYgqga 273  Flavobacteria ba...
AEV32513   211 LNddfngvvftqlevsdyyerkdgr-----------------efknadfykdptgfksvfrhyarTTDIYIPCHFWsnka 273  Owenweeksia hong...
ADF53857   211 LAsefgepvychidvldynkrkdga-----------------vgsiseffinpelyesdfwrfavVSDFFIAGHFYgqga 273  Zunongwangia pro...
EHQ44518   210 LTqkydipvytqlevmdyykrkdgg-----------------eatkadfyvnpeqyesnferftkVTDVLITGHFFkhgs 272  Myroides odoratu...
AEE12903   217 TIrksyetkplfvnlllkdlvqridg---------------kpfsridfsnhpeeyvscllpkleGFPVLIPCHFWgekd 281  Porphyromonas as...

Feature 1                                                                                      
3UHA_A     257 apftn---meklnnpnrRLRTVVDVSADTtnpHNPIPIYTVaTVFNKPTVLVPtta---------gpKLSVISIDHLPSL 324  Saccharomyces ce...
2QRK_A     278 apftn---meklnnpnrRLRTVVDVSADTtnpHNPIPIYTVaTVFNKPTVLVPtta---------gpKLSVISIDHLPSL 345  baker's yeast
2QRJ_A     278 apftn---meklnnpnrRLRTVVDVSADTtnpHNPIPIYTVaTVFNKPTVLVPtta---------gpKLSVISIDHLPSL 345  baker's yeast
NP_567914  326 pcllstkqlqdltkkglPLVGICDITCDI---GGSIEFVNRaTLIDSPFFRFNpsnnsyy-ddmdgdGVLCMAVDILPTE 401  thale cress
AEA45585   277 pfilt---addlkssknKIQVVADVSCDI---QGPIASTLRpSKIADPFYGYDpktgeec-dwkatgSITVMAVDNLPCE 349  Fluviicola taffe...
EEG43351   274 paffk---lkdiqgphfKVSVIADVSCDI---GHPIPTTLRaSTIADPIYGIDretgkei-dfmhpnAIAVMAVDNLPCE 346  Flavobacteria ba...
AEV32513   274 pfvft---redarspefKIRLVSDISCDI---DGPVASTLRpSTIAEPFYAYDphgekev-plgtlgSIGVSAVDNLPCE 346  Owenweeksia hong...
ADF53857   274 pvfft---kedaktpnfNIKYIADISCDI---AGPIPSTIRaSTIADPIYGYDpktgket-nykdpkAITVMAVDNLPCE 346  Zunongwangia pro...
EHQ44518   273 pkilt---ramlnaidnKIQVVGDVSCDVd--HGPIDSTLRaSTIADPLYGYHpgkneev-afdhpaAITVMAIDNLPCE 346  Myroides odoratu...
AEE12903   282 pvyln---qedliklsgKLKLVADVTCDI---SGGFKCTIRsSTHANPFYDYNpfsnkeedafsspsNITIMAVDTLPNA 355  Porphyromonas as...

Feature 1                           
3UHA_A     325 LPREASEFFSHDLLPSLELLP 345  Saccharomyces cerevisiae S288c
2QRK_A     346 LPREASEFFSHDLLPSLELLP 366  baker's yeast
2QRJ_A     346 LPREASEFFSHDLLPSLELLP 366  baker's yeast
NP_567914  402 FAKEASQHFGDILSGFVGSLA 422  thale cress
AEA45585   350 LPKDASEDFGNELIKSVFPHL 370  Fluviicola taffensis DSM 16823
EEG43351   347 LPRDASEGFGAAFLTHVIPAF 367  Flavobacteria bacterium MS024-3C
AEV32513   347 LPKDASEDFGNELIKNVIPHF 367  Owenweeksia hongkongensis DSM 17368
ADF53857   347 LPRDASEGFGEMFLNKVLPAF 367  Zunongwangia profunda SM-A87
EHQ44518   347 LPKDASEGFGDVFLNAILPAF 367  Myroides odoratus DSM 2801
AEE12903   356 IPITSSVYFGSKYMELIIPGL 376  Porphyromonas asaccharolytica DSM 20707

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