2VZ9,2VZ8


Conserved Protein Domain Family
enoyl_red

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cd05195: enoyl_red 
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enoyl reductase of polyketide synthase
Putative enoyl reductase of polyketide synthase. Polyketide synthases produce polyketides in step by step mechanism that is similar to fatty acid synthesis. Enoyl reductase reduces a double to single bond. Erythromycin is one example of a polyketide generated by 3 complex enzymes (megasynthases). 2-enoyl thioester reductase (ETR) catalyzes the NADPH-dependent dependent conversion of trans-2-enoyl acyl carrier protein/coenzyme A (ACP/CoA) to acyl-(ACP/CoA) in fatty acid synthesis. 2-enoyl thioester reductase activity has been linked in Candida tropicalis as essential in maintaining mitiochondrial respiratory function. This ETR family is a part of the medium chain dehydrogenase/reductase family, but lack the zinc coordination sites characteristic of the alcohol dehydrogenases in this family. NAD(P)(H)-dependent oxidoreductases are the major enzymes in the interconversion of alcohols and aldehydes or ketones. Alcohol dehydrogenase in the liver converts ethanol and NAD+ to acetaldehyde and NADH, while in yeast and some other microorganisms ADH catalyzes the conversion acetaldehyde to ethanol in alcoholic fermentation. ADH is a member of the medium chain alcohol dehydrogenase family (MDR), which has a NAD(P)(H)-binding domain in a Rossmann fold of a beta-alpha form. The NAD(H)-binding region is comprised of 2 structurally similar halves, each of which contacts a mononucleotide. The N-terminal catalytic domain has a distant homology to GroES. These proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site, and a structural zinc in a lobe of the catalytic domain. NAD(H) binding occurs in the cleft between the catalytic and coenzyme-binding domains, at the active site, and coenzyme binding induces a conformational closing of this cleft. Coenzyme binding typically precedes and contributes to substrate binding.
Statistics
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PSSM-Id: 176179
View PSSM: cd05195
Aligned: 401 rows
Threshold Bit Score: 129.997
Threshold Setting Gi: 40806909
Created: 23-Aug-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                           #                                                             
2VZ9_A       1560 QDRLCSVY-----YTSLNFRDVMlatgklspdsipgkwltrdcMLGMEFSGRd----------aSGRRVmgmvpaegLAT 1624 pig
XP_958169    1900 DFIDVAVD-----AVGVNSRDLEhwagr-----------vdgdYMTSEYAGVvtavgsrvhniqVGDRVygvg-kgqFGN 1962 Neurospora cr...
XP_001215234 1873 DFIDVEVA-----AVGLNATDLDhwagr-----------vdsnSLSSEYAGVvtavganvahlkVGDRVyglg-kghFGN 1935 Aspergillus t...
XP_001228574 1949 DQIEVQVA-----AAGLSWKDISvasgr----------egatrYLSAEYAGIvtkvgsgvaglsVGDRVygig-kghFGT 2012 Chaetomium gl...
XP_001589519 1580 EDKEFMVKnntfyVSRLSQDPSLdeqfvarve--sepssynseFLGLEGAGVirkigpavtnvaVGDRVswag-lgkFRN 1656 Sclerotinia s...
XP_001801515 1603 DEAEVEVK-----AIGLNMKEVAtfrgn-----------fnslSLSHEGSGIvrrvganvtnvrVGDKVcwma-kglFQN 1665 Phaeosphaeria...
XP_001905772 1903 DYVDVQVA-----AVGVNWKDLGltsgrf---------datgsNLSSEYAGVvtkigaavdglsVGDRVygvg-rgqFGN 1967 Podospora ans...
CAP93159     1736 DWIEIKLE-----AVGLNWKDLGlcsgr-----------fdqnNLSNEYVGVvsdmgssvhdlsIGDRVygmg-kghFGN 1798 Penicillium c...
CAP99663     1922 DEVEIEVK-----AVGLNMKDYViamgn-----------fesvKSSNESTGIisrvgenvthlqPGDKVicle-rgyYDT 1984 Penicillium c...
EED13647     1913 DWIEIKTD-----AIGLNMKDLAvatar-----------fdldKLSTEGAGVvtalgpkvtsvkLGDRVfgii-pgnMGN 1975 Talaromyces s...
Feature 1                                      #                    # ####                  ##   #
2VZ9_A       1625 SVLLlqhatWEVPSTWTLEEAASVpIVYTTAYYSLVVRGrmqpgESVLIHSGSGGVGQAAIAIALSRGcrVFTTVGSaEK 1704 pig
XP_958169    1963 VTRVpaafaTKLQPNDDMIRMASMpLAYTTAVYALDHVAhvregQSILIQSGARDLGLAAITLAKAKRsqVFATVETaEQ 2042 Neurospora cr...
XP_001215234 1936 YTRVpaclsSKLDAADDMVQMASMpVAYMTAIFALDHVAhvrkgQSVLIQSPTTDVALASMRLAKAKGavVVAMVETsEE 2015 Aspergillus t...
XP_001228574 2013 YERVpaalaQRLQPADNLVEAATMpIDFASALYAFDYVTrvrkvHSILVQSAAEGLGLAAIQVARSKGanVFAIARDaDE 2092 Chaetomium gl...
XP_001589519 1657 IERFdsrfvQKLQPKETFEQMSSImIPFVTAVYGLIHLAklqpgESVLIHSATGGVGLAAIQIAKMIGaeIFATVGTpEK 1736 Sclerotinia s...
XP_001801515 1666 IETFkakflHKIADTDSHEEVATMpLAYSTAVYGLLHLGrlkkgEVILIHSGTGGVGLAAIQIAQMVGakVFATVGTeTK 1745 Phaeosphaeria...
XP_001905772 1968 YTRVpaafaQKLEPGDILTEMATMpLVYMTAIYAFDYVArlrkgQKVLLQSATGGLGLAAIQVARARGadVFATVGNaDK 2047 Podospora ans...
CAP93159     1799 YTRVpavlaQKLEAGVDSLEAATMpLVYMTAVYAFEHITrvrkgSKVLIQSASGGLGLAATQLALSKGadVYVTVGTaDK 1878 Penicillium c...
CAP99663     1985 FLRSpvqkcLKLDDNADLVEMATVgIAHGTAIYALDYLAhleanETVLIQAATGGLGLAAIQYARYVGaeIYATVGTqPK 2064 Penicillium c...
EED13647     1976 RLRSpaslvSKIPDGMSSDGAASMpVVYLTSIYALKHLArlergESVLIQSATGGLGMAAIQIAQSLGaeIYATVGTdDK 2055 Talaromyces s...
Feature 1                             ##                           #                       #      
2VZ9_A       1705 RAYLQArfpq--ldetCFANSRdt-sFEQHVLRhta----gkGVDLVLNSLAEe-kLQASVRCLAq-HGRFLEIGKFDls 1775 pig
XP_958169    2043 GKFLVEelglpashviSILDSKqlqrAAEETRKr--------KFEVVMVTAARgelLSLFLQVLAp-LGCLVDIGGVDgq 2113 Neurospora cr...
XP_001215234 2016 ARFLETe--------iAMPASNvcagFTTACLQkaaevapqrGFDIILSTVHGe-fLRSSLQILRp-LGHLIDIGRGDaq 2085 Aspergillus t...
XP_001228574 2093 AAFLADt--------aGVQRDH----IIISSSD---------NLEALTNAARAt--SGRLLNALDskASEKLDMLRNN-- 2147 Chaetomium gl...
XP_001589519 1737 KAFLMQeygl---hddHILSSKda-sFTSDIMRitn----grGVDVSLNSLVHe-qLRATWNSIGh-HGRHIEVGQTDil 1806 Sclerotinia s...
XP_001801515 1746 RNFLHEaygl---phsQILNSRdt-sFAEGVMKatn----grGVDVTLNSLVRe-qLHATWSCIAt-QGRHIEIGLNDil 1815 Phaeosphaeria...
XP_001905772 2048 VSFLVDtvgi---padHIFSSRdptaLSNAAKAtg-----rgGFDVILSTVVGgdfLCESLKALAp-MGHLVDVGRLDvl 2118 Podospora ans...
CAP93159     1879 AQFLSDvmgi---ssdHIFSSRrltdVPRMISAtk-----ngGFDVILSTSQGd-mLYESIKALAp-LGHLIDVGRMDvt 1948 Penicillium c...
CAP99663     2065 KDYLISecgi---padHIFWSRtl-eFKDELFKqtr----grGVDVVLSTISGp-gFHESLKCLAp-CGRLIDVGRGNvl 2134 Penicillium c...
EED13647     2056 VRVLVEefgi---pahRIFNSRrl-sSIDKVMKats----qrGIDVILSSAGGd-sMHEMWRCIAp-LGRFIDVGRTDvl 2125 Talaromyces s...
Feature 1                           ###                                                      ## # 
2VZ9_A       1776 nnhaLGMAVFlknVTFHGILLDSlfee-ggatWQEVSELLKAGIQ-----EGVVQPLKCTVFprtkve--aafryMAQGK 1847 pig
XP_958169    2114 ttpvVGQMLLphnATYASIDPFAvfd----sdPLLGAELMQSVDDyy--rQGVIGPVPRITAadvgq---lssalGDFGT 2184 Neurospora cr...
XP_001215234 2086 ntqaVGMDLPrknATYSSVDASAild----sdLLLAGELMRSVDEyy--rQGLIGPTERITTtnvaq---vsqvlDSFSS 2156 Aspergillus t...
XP_001228574 2148 ----TNFSSF---DIGEILDNDPk-------lGAELMKKVHWLYQ-----AGQISPATPVSVtsvael-dqtllaLSKGT 2207 Chaetomium gl...
XP_001589519 1807 dqgmLDMGPFkrgASFIAMDLVLvfe----qkPDLASQLLSEVIQyy--rGSKIQLLPNISVfpasev-sqafeeFGMRN 1879 Sclerotinia s...
XP_001801515 1816 dygdLDMSVFkrgASFAAFDFGVvad----etPDVAVAVMKEVVGyf--rEGKIKPLSPSSVypasevtkafmqfNNAKR 1889 Phaeosphaeria...
XP_001905772 2119 eakdIGLEHFqrnATLTSFDLNVlld----ndPELGRELMQTVNDly--rWNVIAPIHPFAVhdvsel-drvlvgLSRGT 2191 Podospora ans...
CAP93159     1949 saktVALELFhksASFTSFDLGLvie----rdVELGGDLMFAVNQhf--rAGRIGPIRPYHVsdisql-dqallkLSKGT 2021 Penicillium c...
CAP99663     2135 dkgnMGLHAFdrsISFFSFDLNFvleekpriaERQESQSLSQLLRiellrEGKIHPIRLDNTfhitem-ekalryFGQGQ 2213 Penicillium c...
EED13647     2126 gggrLGLEVFkrnATFSSFDMGLiyr----qdPGLVSKLMTEMIKli--hEGVIGPIKHLTTfcisql-dsalstFSKGL 2198 Talaromyces s...
Feature 1         #      
2VZ9_A       1848 HIGKVVI 1854 pig
XP_958169    2185 VSGKLVA 2191 Neurospora crassa OR74A
XP_001215234 2157 VIGKVVV 2163 Aspergillus terreus NIH2624
XP_001228574 2208 HTGKTVV 2214 Chaetomium globosum CBS 148.51
XP_001589519 1880 RIGRVVF 1886 Sclerotinia sclerotiorum 1980
XP_001801515 1890 CLGGLGR 1896 Phaeosphaeria nodorum SN15
XP_001905772 2192 HIGKLVV 2198 Podospora anserina DSM 980
CAP93159     2022 HIGKMVI 2028 Penicillium chrysogenum Wisconsin 54-1255
CAP99663     2214 HIGKVVL 2220 Penicillium chrysogenum Wisconsin 54-1255
EED13647     2199 HIGKFVI 2205 Talaromyces stipitatus ATCC 10500

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