1E3I,1VJ0,1V3V,3GAZ,1GU7,1HET,1IYZ,1KOL,1O8C,1PL6,1PS0,1QOR,1YB5,2C0C,2CD9,2DQ4,2EIH,2H6E,2J8Z,2VN8,2VZ9,3FBG,3GOH,3GQV,3IP1,3KRT,1YKF,1JQB,2DPH,1D1T,1E3E,1F8F,1MC5


Conserved Protein Domain Family
MDR

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cd05188: MDR 
Click on image for an interactive view with Cn3D
Medium chain reductase/dehydrogenase (MDR)/zinc-dependent alcohol dehydrogenase-like family
The medium chain reductase/dehydrogenases (MDR)/zinc-dependent alcohol dehydrogenase-like family, which contains the zinc-dependent alcohol dehydrogenase (ADH-Zn) and related proteins, is a diverse group of proteins related to the first identified member, class I mammalian ADH. MDRs display a broad range of activities and are distinguished from the smaller short chain dehydrogenases (~ 250 amino acids vs. the ~ 350 amino acids of the MDR). The MDR proteins have 2 domains: a C-terminal NAD(P) binding-Rossmann fold domain of a beta-alpha form and an N-terminal catalytic domain with distant homology to GroES. The MDR group contains a host of activities, including the founding alcohol dehydrogenase (ADH) , quinone reductase, sorbitol dehydrogenase, formaldehyde dehydrogenase, butanediol DH, ketose reductase, cinnamyl reductase, and numerous others. The zinc-dependent alcohol dehydrogenases (ADHs) catalyze the NAD(P)(H)-dependent interconversion of alcohols to aldehydes or ketones. ADH-like proteins typically form dimers (typically higher plants, mammals) or tetramers (yeast, bacteria), and generally have 2 tightly bound zinc atoms per subunit, a catalytic zinc at the active site and a structural zinc in a lobe of the catalytic domain. The active site zinc is coordinated by a histidine, two cysteines, and a water molecule. The second zinc seems to play a structural role, affects subunit interactions, and is typically coordinated by 4 cysteines. Other MDR members have only a catalytic zinc, and some contain no coordinated zinc.
Statistics
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PSSM-Id: 176178
View PSSM: cd05188
Aligned: 199 rows
Threshold Bit Score: 84.2955
Threshold Setting Gi: 7416785
Created: 8-Nov-2004
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
NAD(P) binding
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:1E3E: Mouse class II alcohol dehydrogenase binds NAD, contacts at 4A
    View structure with Cn3D
  • Structure:1DIT: Human alcohol dehydrogenase binds NAD, contacts at 4A
    View structure with Cn3D
  • Citation:PMID 9228021
  • Structure:1MC5: Human class III alcohol dehydrogenase binds NAD, contacts at 4A
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  • Structure:1F8F: Acinetobacter calcoaceticus benzyl alcohol dehydrogenase binds NAD, contacts at 4A
    View structure with Cn3D
  • Structure:1YKF_A: Thermoanaerobacter brockii NADP-Dependent alcohol dehydrogenase binds NADP, contacts at 4A
    View structure with Cn3D
  • Structure:2DPH: Pseudomonas putida formaldehyde dismutase binds NAD, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                ###  #                                                               
1E3I_A     35 EVRIQviATCVCPTDINatdpkk----kalfpvVLGHECAGivesvgp--gvtnfkPGDKVipffapqckrcklclsplt 108 house mouse
1PS0_A     35 DIDIKieACGVCGSDIHcaaghwg---nmkmplVVGHEIVGkvvklgp-ksnsglkVGQRVgvgaqvfsclecdrckndn 110 baker's yeast
3GOH_A     31 DILVQnqAIGINPVDWKfikanpi---nwsnghVPGVDGAGvivkvga--kvdskxLGRRVayhtsl------------- 92  Shewanella oneiden...
NP_342271  27 EVVIRvnRAALCYRDLLqlqgfyp---rmkypvILGHEVVGtieqige--nakgfkEGDKVisllyapdgtchycqmge- 100 Sulfolobus solfata...
YP_794990  29 HSVMKihAFGVHRYEVLtreggsp---tvkfprVIGVEAVGeihett---agsgfkQGQRVitlnggf------------ 90  Lactobacillus brev...
EEB12619   55 QIRIKvqYCSVNPSDVEivkgyhe--ykpklpfVPGYEMAGeitevgkdaklngfnVGDKViglnk-------------- 118 human body louse
YP_644819  30 ETLIRlhAAALNRRDLFvtygqypgarpealpvILGSDGSGevvahgd-gaetlppEGSEViinpalhwgedqtkp---- 104 Rubrobacter xylano...
YP_707829  31 DAVLAlsAAGLNRHELFlvnnrt----gtepplILGADGVGtvtqvgs--pahaalVGTTVlidpcigwpddtql----- 99  Rhodococcus sp. RHA1
NP_832416  29 EVKIKlkAAGLNHRDLFiinnrk----emdlplVIGSDGSGivteige--gvsnitLHTEViinpsigwehatev----- 97  Bacillus cereus AT...
BAC13332   26 EVVVKlkAAGLNRRDLAipkrrg----deqealILGSDGAGvidsigd--evenvqVGDEViinpslrwkqnsdap---- 95  Oceanobacillus ihe...
Feature 1                                                                            #   #    
1E3I_A    109 nlcgklrnfkyptidqelmedrtsrftckgrsiyhfmgvssFSQYTVVseanlARVDDean--lerVCLigCGFSSGYGA 186 house mouse
1PS0_A    111 epy-----------------ctkfvttysqpyedgyvsqggYANYVRVhehfvVPIPEnip--shlAAPllCGGLTVYSP 171 baker's yeast
3GOH_A     93 ------------------------------------krhgsFAEFTVLntdrvXTLPDnls--ferAAAlpCPLLTAWQA 134 Shewanella oneiden...
NP_342271 101 ------------------------eaychsrvgyseeldgfFAEKAKVkv--tSIVKVgtnvpdegAVLvpCVTGMVYRS 154 Sulfolobus solfata...
YP_794990  91 ----------------------------------grefdgsEQEYALVpnktlYTVDFdgd--wvtLAQypETFVTAWGS 134 Lactobacillus brev...
EEB12619  119 ------------------------------------escggFAEECVVvskdcYHVPQgisl-kecSALl-DGYITALLG 160 human body louse
YP_644819 105 ---------------------------greyrilglpddgtFAQLVKVptenvFRKPAhlt--heqAAAfpLAALTAYRA 155 Rubrobacter xylano...
YP_707829 100 ---------------------------pevpeilggprpgtFAQRITVplnniHPVPPhlt--rheAAAlgLSASTAYRA 150 Rhodococcus sp. RHA1
NP_832416  98 ---------------------------pelpevlggpkdgtFAEYVIVpaenvVEKPSylt--weeSGVlsLSALTAYRA 148 Bacillus cereus AT...
BAC13332   96 ---------------------------pkgfdilgmpdhgtIAEKIVIasdqvEPKPSyls--weeAAVfsLAALTGYRA 146 Oceanobacillus ihe...
Feature 1                     #### ##                  ##   #              #                  
1E3I_A    187 AintakvtpgstCAVFGLGC-VGLSAIIGCkiagasriIAIDIngeKFPKAKalgatDCLNPreldkpvqdviteltagg 265 house mouse
1PS0_A    172 Lvrngc-gpgkkVGIVGLGG-IGSMGTLISkamg-aetYVISRssrKREDAMkmgadHYIATleegdwge-----kyfdt 243 baker's yeast
3GOH_A    135 Fekipl-tkqreVLIVGFGA-VNNLLTQXLnnag-yvvDLVSAs-lSQALAAkrgvrHLYREpsqvt-----------qk 199 Shewanella oneiden...
NP_342271 155 Lrrakl-qkgetVLVTGASGgVGIHAIQVAkalg-akvIGVTTs-eEKAAFIrkfadHVIVGskfseeak------kigd 225 Sulfolobus solfata...
YP_794990 135 Ikalrl-qpgqtMLVRGGTSaVGLAMIKLAkasg-lkiVATTRkpeRADLLSaqgadEVVLDkdgqlqt--------dtm 204 Lactobacillus brev...
EEB12619  161 LvrrgkleegetVLVTAAGGgVGLAAVDLAanvykakvIAVCStedKAALVRekgafMALKYddhlvkevqn--ftngkg 238 human body louse
YP_644819 156 LvtrggvregetVVITGAGGgVATFLVQLAhalg-arvFVTSGsdeKLRKARelgaeGGVNYrsenwarelk--eltggg 232 Rubrobacter xylano...
YP_707829 151 LftrgglqpsehVMITGISGgVGPLALAFAvaagaevtVITRRh-dSADRARqlgaaHAIVGaddfeea-------ltrr 222 Rhodococcus sp. RHA1
NP_832416 149 LftkgrlkcgehVLIPGIGGgVATFAMLFAkaig-akvSVTSRvenKRELAEkygadISFNStgnweesl------qgek 221 Bacillus cereus AT...
BAC13332  147 IftkgklqpgetVFIPGAGSgVASFLIHFAknhg-arvITTSRskdKQEKAKdlgadVVLDTsddweaal------qdet 219 Oceanobacillus ihe...
Feature 1           ##                        ##                        ###                   
1E3I_A    266 vdySLDCAgta--qtLKAAVDctvlgwGSCTVVGakvdemtip---tvdvilgrSINGTFFggwks----------vdsv 330 house mouse
1PS0_A    244 fdlIVVCAssltdidFNIMPKamk-vgGRIVSISipeqhemls--lkpyglkavSISYSALgsik-------------el 307 baker's yeast
3GOH_A    200 yfaIFDAVns---qnAAALVPslk-anGHIICIQdripapid-----paftrtiSYHEIALgalhdfgdrqdwqilxqqg 270 Shewanella oneiden...
NP_342271 226 vnvVIDTVgt---ptIDESLKslw-mgGRLVQIGnvdptqiyqlrlgyvilkdiAIIGHASatkr-------------da 288 Sulfolobus solfata...
YP_794990 205 ydgIVDLVgt---atVHDSLAhik-vgGTACVVGllagdwvmkd-fspfelqnrYITDYDSgfvdq-----------pmi 268 Lactobacillus brev...
EEB12619  239 vkvVFDATgd---kvFESALNcvs-neGRMVLASnaarniknfn-lssllpkavSITGVSPwhyrindi----etykscv 309 human body louse
YP_644819 233 adlSVDSVgg----eVFNALVslakpgSRIVTFGatagpvpklv-lpriflkhlTVLGTAMgsnv-------------df 294 Rubrobacter xylano...
YP_707829 223 adlVVDSVgg---svFGSALRalr-pgGRVVTFGattgadvpis-lrdlffrqiDIRGTSMgnah-------------df 284 Rhodococcus sp. RHA1
NP_832416 222 vdlIIDSIgp---atFLKYFDvlk-pnGRIVNFGassgdkielp-lralfynqiDIMGTSMgsre-------------ef 283 Bacillus cereus AT...
BAC13332  220 idmVIDSVgk---atFHRSLAvlk-kgGRFVTFGattedvidfd-lrsffygqyQLIGTTMgcke-------------el 281 Oceanobacillus ihe...
Feature 1            
1E3I_A    331 pNLVSDY 337 house mouse
1PS0_A    308 nQLLKLV 314 baker's yeast
3GOH_A    271 eALLTLI 277 Shewanella oneidensis MR-1
NP_342271 289 eETLKLT 295 Sulfolobus solfataricus P2
YP_794990 269 dELFDLI 275 Lactobacillus brevis ATCC 367
EEB12619  310 eDVIDMA 316 human body louse
YP_644819 295 eGALQLY 301 Rubrobacter xylanophilus DSM 9941
YP_707829 285 rAMLEFV 291 Rhodococcus sp. RHA1
NP_832416 284 nEMIKFI 290 Bacillus cereus ATCC 14579
BAC13332  282 eAALNHY 288 Oceanobacillus iheyensis HTE831

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