Conserved Protein Domain Family
RasGAP_CLA2_BUD2
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cd05137: RasGAP_CLA2_BUD2 
Ras-GTPase Activating Domain of CLA2/BUD2
CLA2/BUD2 functions as a GTPase-activating protein (GAP) for BUD1/RSR1 and is necessary for proper bud-site selection in yeast. BUD2 has sequence similarity to the catalytic domain of RasGAPs, and stimulates the hydrolysis of BUD1-GTP to BUD1-GDP. Elimination of Bud2p activity by mutation causes a random budding pattern with no growth defect. Overproduction of Bud2p also alters the budding pattern.
Links
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Statistics
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PSSM-Id: 213339
Aligned: 53 rows
Threshold Bit Score: 318.354
Created: 31-May-2007
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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putative RAS
Feature 1:putative RAS interface [polypeptide binding site]
Evidence:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                        #                                
395289        460 KISSNLNFILPSTNFVKLEKLLMNANLSmvsnLIYKSSSSmen-dnkLTQTSIIFLDIFQSLSRIEEWFHVLIDKELaki 538  baker's yeast
XP_503210     579 KISYFGSNVLAPHQYEHLEAMLKDTDSRls-aKISEARMS-------LPEYTDMFVKIYSCLGICDQWLMSLIDGEIgkl 650  Yarrowia lipo...
CCA39503      423 NLKQSVNYILNPSYFKFVEKVLNRLNPR----EMIDFIKPltglnqdLEEISSIVLDVYQVLGTENSWFAALMESELtpi 498  Pichia pastor...
EGU13145      842 SLKINEEVVLPSRDYQEILNLLTDDPEAdlasEIAHEFPAd------LEEVTRILLRVYSAKSLLLPRVLRLADLEIdsn 915  Rhodotorula g...
EFW97602      423 NLSLREIHIMSYKNYRMFEGMMKGIDVRdllrFVTPLIEPq-----nVDDLSQMLLDIYQSANMEHEYFEQLMSFELesl 497  Pichia angust...
XP_003330309 1094 SITVIEQVVLAEPEYAKMMNYLNNDDDIvlplDLANMAVGe------LDRLAELLIRIYEANNRLFMRFSQLAAIEIngd 1167 Puccinia gram...
XP_003330333 1192 SITVIEQVVLAEPEYAKMMNFMNNDNDIelplNLAHMAVGe------LDRLAELLIRIYEANNRLILRFSQLAAIEIngd 1265 Puccinia gram...
XP_001828717  324 KLRVDEEIILPQAAYQGLLATFDSRNFLd---WMTELESKl-----kLKHISSQLMAVAIAQDTLIVQVQEFAAREVlga 395  Coprinopsis c...
EGO29899      584 KMRVDEDIILPYSAYSGLLQARNILDWM----DDFEHKLH-------LRSISSELMYVAIAKNVLLDQVFELADREVdgt 652  Serpula lacry...
CBQ69456      618 SIRLHEGAVMPLSMYDEVYRRIHDADAA----EMVRHLSTtm----rEDLIISHLIRIYAASGTIAERISSLIEAESvsw 689  Sporisorium r...

Feature 1                                        # # ## #  #   #                                  
395289        539 dgtvsrin-------------qknldskhvfNSLFRGNSILTKSIEQYFFRVGNEYLSKALSAILKEIiesnkscELDPA 605  baker's yeast
XP_503210     651 adkvta-----------------ndrskqvsNLLFRGNTLMTKAIEKYMQLTAHSYLRDLVGKVVRQVldrdeklELDPS 713  Yarrowia lipo...
CCA39503      499 dsitrnnriiiahtangttdtenlihtknafNTLFRGNSILSKSLEKYGLRIGHEYLEKVIGSFVKRLteekkdcEVDPR 578  Pichia pastor...
EGU13145      916 ar------------------------nnrsaAILFRGNTILTKTIELYLRLIGAEYLDASIGNVIRRIcadkveiEIDPM 971  Rhodotorula g...
EFW97602      498 knsgskr---------------nstsltnnlNTLFRGRSLLSTSLEKYQIRIGQEYLEKLLGEFIAQIcae--nlICDPN 560  Pichia angust...
XP_003330309 1168 i---------------------------staSILFRANTLLTKMLEAYMRIVGSSFLESSVGAVIRRIcmakaelEIDPS 1220 Puccinia gram...
XP_003330333 1266 l---------------------------staSILFRANTLLTKMLEAYMRLVGRSFLESSIGPVVRRIciaktelEIDPA 1318 Puccinia gram...
XP_001828717  396 p---------------------------ishQTLFRGNTVFTKVMELAMTWYGKAFLEASIGQVLRRLcaekvaiEVDPV 448  Coprinopsis c...
EGO29899      653 p---------------------------rshNTLFRGNTILTKTIELCMAFYGKAFLEASIGSSIRRLcsekiaiEVDPA 705  Serpula lacry...
CBQ69456      690 ge-------------------------kmepELLFRANTLLSRSVDHFQRLLALSWLDDCLGPTVRKIch----dPLAPS 740  Sporisorium r...

Feature 1                                                                                         
395289        606 RVKekde------------------------------vkkrKIIADNYKRLYSWVTKIWKRLYATSNDLPIEIRNVLKIF 655  baker's yeast
XP_503210     714 RLDdghgi----------------------------tpeieQLKVHNQERLMAHAETIWVNIRNTIADIPQSLVVVFQYL 765  Yarrowia lipo...
CCA39503      579 MIEipksaemdd---------------------etvkqkrqEQAEDNMKNLVEYVSILWDQLYNTTNDIPTPLKEQWKNL 637  Pichia pastor...
EGU13145      972 KVKpgt---------------------------------kdKDLLANTHALHEWTLTLWNSIYDAREKCPHDLRQIFNHI 1018 Rhodotorula g...
EFW97602      561 PKTy------------------------------------pNTHEEHYQNLLNYVEELWNRIYTTSNDLPEELKREWGRL 604  Pichia angust...
XP_003330309 1221 KLKtslss-----------------------------hhkeKIVNENAKELKRLSSEIWQSMYINRSKCPNRLRKIFVKI 1271 Puccinia gram...
XP_003330333 1319 KLKpslsa-----------------------------hqkeKIVTENVKELKKICNEIWQSMYINRSKCPNRLRKIFVKI 1369 Puccinia gram...
XP_001828717  449 KSGks-----------------------------------nKEVEKNVEQLIYWCQEFWNQIYAVRNECPIELRRLFLTI 493  Coprinopsis c...
EGO29899      706 RSGks-----------------------------------tKDTERDVELLIYWCKEFWNQIYAVRNECPNEMRKLFEHV 750  Serpula lacry...
CBQ69456      741 RPDtatssssagsagndeshfdfstsmrsppslpsnaldaiPDGPMTTNALRKLSENMWQNIYRQRHCCPPDLRTVLHQI 820  Sporisorium r...

Feature 1                                #       ##  ##  #                    #  ##  #   # #      
395289        656 RQKLEIICIDd------tLQIILNGISGLLFLRFFCPVILNPKLFKYVSQNLNETARRNLTLISKVLLNLSTLTQFA--N 727  baker's yeast
XP_503210     766 RQQLEGLHQD--------EEVVFNGISGFLFLRFICPALLSPKLFGLVHNSPQPEVQRTLTLLAKLMQSFANRANFT--A 835  Yarrowia lipo...
CCA39503      638 RHKVEQAVEPs------dTETPLNALSSFVFLRFICPTVLNPKLFYLTKDHQVGMVGRTLTLIAKILLTIANRSSFVe-H 710  Pichia pastor...
EGU13145     1019 QTVVVEKYGQsd----dqKNTRWTCVSAFIFLRFFVPAVLNPKLFFIVSNPPDPKAQRTLTLVAKTLQGLANFSSFG--Q 1092 Rhodotorula g...
EFW97602      605 RSKVEHSVDPn------dTETPLNALCSFIFLRFLCPAILNPKLFNLSKNHQRGRVSKTLTLIAKVLMTLANRSQFAp-H 677  Pichia angust...
XP_003330309 1272 QELVGNAYDD--------QDMRLTSISAFVFLRFFVPAILNPRLFNLIQFQPDSKSQRTLTLVAKTLQGLGNLTLFG--I 1341 Puccinia gram...
XP_003330333 1370 QELVGNAYAD--------QDMRLTSISAFVFLRFFVPAILNPRLFSLIQFQPDGKSQRTLTLIAKTLQGLGNLTLFG--I 1439 Puccinia gram...
XP_001828717  494 RKLVEERCRNengsiganRDLPKQSVSAFVFLRFIVPAILHPHLFGLCPGLPSDPVKRSLTLIAKVIQSLANLNASA--Q 571  Coprinopsis c...
EGO29899      751 RKLVEKRYSRnnmpeeqsWDLPWQSVSAFCFLRFIVPAILHPHLFGLYPGLPTMPVQRSLTLIAKVIQSLANLNASV--Q 828  Serpula lacry...
CBQ69456      821 RCKVNERYRSsk-----sTRPGIQGVGAFVFLRLFCAALNAPQLYGLTPSQPGRGAQRKLLLLSKVLLALASKKTAFdkD 895  Sporisorium r...

Feature 1         ##                                                                              
395289        728 KEPWLMKM-NNFIDKRHNDLLDYIDKMTQkk------ldFNSKILNLSstis-------------rpklAIEQTMLDDLP 787  baker's yeast
XP_503210     836 KESFMIPL-TPFIENNKKELCQYFRVISNqn------kwDEIRMAKPSkpqyk------------yqqhDIPLRPLPDAP 896  Yarrowia lipo...
CCA39503      711 KEPHLLPL-NTFIKENNEKVIQYYDKITFrk------ldFSPKVLEMSddvd-------------rpplNIPDWIREELP 770  Pichia pastor...
EGU13145     1093 KEPWMLPM-NAFVRENHAALVDFLEHVSTpgssavlrqeWTSPNAPAYlapy-------------rlrsSLSPLAQEGVP 1158 Rhodotorula g...
EFW97602      678 KDPELLRLnEDFFDKRNDELRLYCDRISGrk------mdFTEKKLEMTnaig-------------kpslTASEEILSELP 738  Pichia angust...
XP_003330309 1342 KEPWMSVM-NEFITTQLDSFRDYISFIASesds--tkpeWTSKEYEGYglpy-------------alraSLPASSRDGIP 1405 Puccinia gram...
XP_003330333 1440 KEPWMSVM-NEFITTQLDSFRDYISFIASesds--tkpeWTSKEYEGYglpy-------------alraSLPSSSRDGIP 1503 Puccinia gram...
XP_001828717  572 KEAFMRGI-KDFLANSLSAMVDYIAAVSGp---------IDEHQANHSnrehrhgrlr-ianglrerraTMAVLDREAIP 640  Coprinopsis c...
EGO29899      829 KEDFMRGV-KAFLKESVPAMVKYIQAVSTpvvgd-thglQSGPAHTHArlnvvns--------lhqrgaTMPALEREAIP 898  Serpula lacry...
CBQ69456      896 KDWELVPL-NDLLRTYSSAYDDYISVVST----------EPPAAAPVQllcvridgdadlqaaalrrlgSLSPLHREAIP 964  Sporisorium r...

Feature 1                                                   
395289        788 QIPYLLDKNLRETEFVN----------LIVNFSQEDMTKMEK 819  baker's yeast
XP_503210     897 TLPYLYDKYACYQELVE----------HWLTTVPTSRVNGVG 928  Yarrowia lipolytica CLIB122
CCA39503      771 TMPYLIDKYLRLTELFT----------IGVLEFEKEGDGSGS 802  Pichia pastoris CBS 7435
EGU13145     1159 LLPHLVDLPRELGALAAr---------VARLAAEKGPIVEES 1191 Rhodotorula glutinis ATCC 204091
EFW97602      739 SRSYLIDKYLRLAEFAS----------LLDSKNSENIVSKES 770  Pichia angusta DL-1
XP_003330309 1406 TLPHLLDPVRDCSLLASlitiikrkasVLDSSRHHSSVLPPK 1447 Puccinia graminis f. sp. tritici CRL 75-36-700-3
XP_003330333 1504 TLPHLLDPVRDCSLLAS----------LMTIINKKASGSSSS 1535 Puccinia graminis f. sp. tritici CRL 75-36-700-3
XP_001828717  641 TLPHLLDVPRHLAVITSa---------VIRSCRDPSIHPGLS 673  Coprinopsis cinerea okayama7#130
EGO29899      899 LLPHVLDIPRHLAVISSa---------VIRSVRFADHKQKMF 931  Serpula lacrymans var. lacrymans S7.9
CBQ69456      965 RAPHMLDQPQALASFVC----------FVADAAQEHEHALDI 996  Sporisorium reilianum SRZ2

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