Conserved Protein Domain Family
ACT_AK-Hom3_1

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cd04934: ACT_AK-Hom3_1 
CT domains located C-terminal to the catalytic domain of the aspartokinase (AK) HOM3, a monofunctional class enzyme found in Saccharomyces cerevisiae, and other related ACT domains
This CD includes the first of two ACT domains located C-terminal to the catalytic domain of the aspartokinase (AK) HOM3, a monofunctional class enzyme found in Saccharomyces cerevisiae, and other related ACT domains. AK is the first enzyme in the aspartate metabolic pathway, catalyzes the conversion of aspartate and ATP to aspartylphosphate and ADP, and in fungi, is responsible for the production of threonine, isoleucine and methionine. S. cerevisiae has a single AK, which is regulated by feedback, allosteric inhibition by L-threonine. Recent studies shown that the allosteric transition triggered by binding of threonine to AK involves a large change in the conformation of the native hexameric enzyme that is converted to an inactive one of different shape and substantially smaller hydrodynamic size. Members of this CD belong to the superfamily of ACT regulatory domains.
Statistics
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PSSM-Id: 153206
View PSSM: cd04934
Aligned: 5 rows
Threshold Bit Score: 121.794
Threshold Setting Gi: 46096389
Created: 18-Apr-2006
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative
Feature 1:putative threonine allosteric regulatory residues
Evidence:
  • Comment:The A-406-T point mutation in HOM3 increases the sensitivity to the allosteric regulator, threonine.
  • Comment:The S-399-F Saccharomyces cerevisiae HOM3 mutant contains an AK activity insensitive to inhibition by threonine.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                          #      #                                   
NP_010972 362 IFVINIHSNKKTLSHGFLAQIFTILDKYKLVVDLISTSEVHVSMALPIpdad-----------slksLRQAEEKLRILGS 430 baker's yeast
O60163    358 IMVINIQSNRKISAHGFLASIFAILDKYKLAVDLITTSEVHVSMALYEesd-------------dgnMHEAFVELRRLGT 424 fission yeast
EAL92034  386 ILVINVHSNKRSLSHGFFAGIFSVLDRWRLSIDLISTSEVHVSMALHSempllngvgrdeyqiidedLKGALNDLQKYGT 465 Aspergillus fumiga...
EAK81622  483 VLVLNVHSNRKTVSHGFFAKIFGTLDKYGVVVDLISTSEVHVSMAMAAalr-------------prtLEKVKADLQKVGT 549 Ustilago maydis 521
CAD36978  380 ISIINIHSNKRSIAHTFLARLFAILNKHRVSVDLISTSEVHVSVAVHMgnae------------mtnFDAAVKDLGDCGD 447 Neurospora crassa
Feature 1          
NP_010972 431 VDITK 435 baker's yeast
O60163    425 LDILH 429 fission yeast
EAL92034  466 VDIIP 470 Aspergillus fumigatus Af293
EAK81622  550 VSVLT 554 Ustilago maydis 521
CAD36978  448 VSVLH 452 Neurospora crassa

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