1PHZ


Conserved Protein Domain Family
ACT_PAH

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cd04931: ACT_PAH 
Click on image for an interactive view with Cn3D
ACT domain of the nonheme iron-dependent aromatic amino acid hydroxylase, phenylalanine hydroxylases (PAH)
ACT domain of the nonheme iron-dependent aromatic amino acid hydroxylase, phenylalanine hydroxylases (PAH). PAH catalyzes the hydroxylation of L-Phe to L-Tyr, the first step in the catabolic degradation of L-Phe. In PAH, an autoregulatory sequence, N-terminal of the ACT domain, extends across the catalytic domain active site and regulates the enzyme by intrasteric regulation. It appears that the activation by L-Phe induces a conformational change that converts the enzyme to a high-affinity and high-activity state. Modulation of activity is achieved through inhibition by BH4 and activation by phosphorylation of serine residues of the autoregulatory region. The molecular basis for the cooperative activation process is not fully understood yet. Members of this CD belong to the superfamily of ACT regulatory domains.
Statistics
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PSSM-Id: 153203
View PSSM: cd04931
Aligned: 6 rows
Threshold Bit Score: 149.192
Threshold Setting Gi: 2464955
Created: 7-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative L-Pheautoregulatory
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:putative L-Phe binding site [chemical binding site]
Evidence:
  • Comment:predicted to be the allosteric/regulatory L-Phe binding site, based on mutational analyses and sequence similarity to prephenate dehydratase

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                 ####                ####                               
1PHZ_A        21 ETSYIEDnsnqngaISLIFSLKEEVGALAKVLRLFEENDINLTHIESRPSRlnkDEYEFFTYLDkrtKPVLGSIIKSLRN 100 Norway rat
129973        21 ETSYIEDncnqngaISLIFSLKEEVGALAKVLRLFEENDVNLTHIESRPSRlkkDEYEFFTHLDkrsLPALTNIIKILRH 100 human
NP_001001298  15 ESTYTEEpsnkngvISLIFSLKEEVGALAKVLRTFEEKGINLTHIESRPSRlnkDEYEFFINLEgknVPALDKIIKSLRN 94  chicken
AAH96008      15 EVNGIENnqe-dnrISLIFSLKEEVGALAKVLRIFEEKDISLTHIESRPSRlnkDEYQFFINLDdksTHALDDIISCLRN 93  western clawed ...
AAP82284      19 TSSYLEEpknksgvVSCIFSLKEEVGALVKALKLFEEKGINLTHIESRPSRknnEEYEFFISVDqasSKVLDEIVDGLRT 98  zebrafish
2464955        8 TGSYLKEgdi-agtMEVIFSVNEEVGALAKALRLFESHKINLTHIESRPSKrnrDQYEFFVRFDp-nAAGVKPALEALRS 85  Florida lancelet
Feature 1                  
1PHZ_A       101 diGATVHELS 110 Norway rat
129973       101 diGATVHELS 110 human
NP_001001298  95 diGVTVHELS 104 chicken
AAH96008      94 diGATVHELS 103 western clawed frog
AAP82284      99 qiRGQVHELS 108 zebrafish
2464955       86 s-AAPYRCIA 94  Florida lancelet

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