2F1F,2F1F


Conserved Protein Domain Family
ACT_AHAS

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cd04878: ACT_AHAS 
Click on image for an interactive view with Cn3D
N-terminal ACT domain of the Escherichia coli IlvH-like regulatory subunit of acetohydroxyacid synthase (AHAS)
ACT_AHAS: N-terminal ACT domain of the Escherichia coli IlvH-like regulatory subunit of acetohydroxyacid synthase (AHAS). AHAS catalyses the first common step in the biosynthesis of the three branched-chain amino acids. The first step involves the condensation of either pyruvate or 2-ketobutyrate with the two-carbon hydroxyethyl fragment derived from another pyruvate molecule, covalently bound to the coenzyme thiamine diphosphate. Bacterial AHASs generally consist of regulatory and catalytic subunits. The effector (valine) binding sites are proposed to be located in two symmetrically related positions in the interface between a pair of N-terminal ACT domains with the C-terminal domain of IlvH contacting the catalytic dimer. Plants Arabidopsis and Oryza have tandem IlvH subunits; both the first and second ACT domain sequences are present in this CD. Members of this CD belong to the superfamily of ACT regulatory domains.
Statistics
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PSSM-Id: 153150
View PSSM: cd04878
Aligned: 59 rows
Threshold Bit Score: 71.0048
Threshold Setting Gi: 1857047
Created: 1-Aug-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative valinedimer interface
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:putative valine binding site [chemical binding site]
Evidence:
  • Comment:based on similarity to 3PGDH and properties of mutants, the effector (valine) binding sites are proposed to be located in two symmetrically related positions in the interface between a pair of ACT domains.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             # #    #            ##    #        #                              
2F1F_A        4 RILSVLLENESGALSRVIGLFSQRGYNIESLTVAptddptLSRMTIQTVGdekVLEQIEKQLHKLvDVLRVS 75  Escherichia coli
3041696      78 HVLNCLVQNEPGVLSRVSGTLAARGFNIDSLVVCntevkdLSRMTIVLQGqdgVVEQARRQIEDLvPVYAVL 149 baker's yeast
CAB91255     94 HILNCLVQNEPGVLSRVSGILAARGFNIDSLVVCntevddLSRMTIVLTGqdgVVEQARRQLEDLvPVWAVL 165 Neurospora crassa
EAL17314     90 HRLNCLVQNEPGVLSRVSGILAGRGFNIDSLVVCqteirdLSRMCIILKGqdgVIEQARRQLEDLvPVWAVL 161 Cryptococcus neoformans ...
CAA18422     71 HVFNCLVQNEPGVLSRLSGILAARGFNIDSLVVCatevenLSRMTIVLRGadeVVEQAKRQIEDIvSVWAVL 142 fission yeast
AAW24461     36 HVIAALVVNQPGCLAEIANLFAARGYNIDSLVVGrteveeLSRMTVVVNGtaqSVVNMKKQLEDVvYVAVVN 107 potato late blight agent
CAD76501     23 HLLSALVQNVPGVLAHISGMLASRGYNIDSLAVGetedptLSRMTFVVVGddqVLDQVRKQLEKIvTVVRVL 94  Rhodopirellula baltica SH 1
ZP_01690810   6 FTVTVFSEDNIGLLNRITDIFTRRKINIDSLTTSasetegIFRFTVVISTtlaNVKRIVKQLEKQvEVLKAF 77  Microscilla marina ATCC ...
ZP_01721220   4 YTIFVITENFIGILNRITIIFTRRGINIDALTASesridgVHRITIEVTAnedTVIQLVNQIEKViDVIKAF 75  Algoriphagus sp. PR1
ZP_01734672  17 FTLTIYTENHVGLINKIAIMFSRRKISLESFNTSpsevpnIYRFTIVVIEaeaVVKNLVKQIEKIvDVFKVY 88  Flavobacteria bacterium ...

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