1QO2,2AGK,1VZW,1OX5


Conserved Protein Domain Family
HisA_HisF
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cd04723: HisA_HisF 
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Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase (HisA) and the cyclase subunit of imidazoleglycerol phosphate synthase (HisF). The ProFAR isomerase catalyzes the fourth step in histidine biosynthesis, an isomerisation of the aminoaldose moiety of ProFAR to the aminoketose of PRFAR (N-(5'-phospho-D-1'-ribulosylformimino)-5-amino-1-(5''-phospho-ribosyl)-4-imidazolecarboxamide). In bacteria and archaea, ProFAR isomerase is encoded by the HisA gene. The Imidazole glycerol phosphate synthase (IGPS) catalyzes the fifth step of histidine biosynthesis, the formation of the imidazole ring. IGPS converts N1-(5'-phosphoribulosyl)-formimino-5-aminoimidazole-4-carboxamide ribonucleotide (PRFAR) to imidazole glycerol phosphate (ImGP) and 5'-(5-aminoimidazole-4-carboxamide) ribonucleotide (AICAR). This conversion involves two tightly coupled reactions in distinct active sites of IGPS. The two catalytic domains can be fused, like in fungi and plants, or peformed by a heterodimer (HisH-glutaminase and HisF-cyclase), like in bacteria.
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Statistics
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PSSM-Id: 240074
Aligned: 36 rows
Threshold Bit Score: 163.98
Created: 10-Jul-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active site
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Position of one catalytic Asp residue is shifted in eukaryotic enzymes
  • Structure:2AGK; Yeast His6 with bound citrate and ethylene glycol
  • Structure:1OX5; Yeast imidazole glycerolphosphate synthase
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             #                                                     #                   
1QO2_B        2 LVVPAIDLfrGKVARXIKGrkent--------------ifyekDPVELVEKLIeegFTLIHVVDLSnaiensgeNLPVLE 67  Thermotoga maritima
2AGK_A        2 KFIGCIDLhnGEVKQIVGGtltskkedv------pktnfvsqhPSSYYAKLYKdrdVQGCHVIKLGp------nNDDAAR 69  baker's yeast
CAA93307      8 LFRPCIDIhqGQVKQIVGGtlgndadv--------ktnyvslkPSSYYAELYKlnhLEGAHVIMLGp------nCEEAAK 73  fission yeast
CAG34734      2 KFRPCIDLhnGQVKQIVGStlsdvepsq------lqtnytadkPASWFANLYRqddLRGGHIIKLGp------gNDDAAK 69  Desulfotalea psy...
BAD72794     45 QFRPCIDIhkGKVKQIVGStlsdlkedgs----vlvtnfesdkSAEEYAKMYKedgLTGGHVIMLGad----plSQAAAI 116 thale cress
XP_568820     7 QFRPCIDLhqGVVKQIVGGtldltsqsga----gptenfvathPPSYFADLYKtndLVGGHIIKLGp------gNDEAAK 76  Cryptococcus neo...
XP_956663     3 RFRPCIDLhaGQVKQIVGGtldsatsel-------rtnfvsphPPAYFAKLYRdndLSGAHVIMLGp------gNKEAAL 69  Neurospora crass...
ZP_01077238   3 KFRPCIDLhqGKVKQIVGGslndhgak---------enfisehDAAYYAALYKehaLTGGHVIALGp------gNKEEAI 67  Marinomonas sp. ...
EAT03275      2 KFRPCIDLhqGRVKQIVGStlggdeagl-------ainftseqPPAYYARLYRrdnLSGGHVIMLGp------gNEAAAT 68  delta proteobact...
CAL54280      9 RFRPCIDIhnGKVKQIVGStlrdgtrpgdaasetpatnfeteiPSTAYAEMYKrdgIYGGHAILLSkd----eaTTRAAM 84  Ostreococcus tauri

Feature 1                                                                           #           
1QO2_B       68 KLSef-aeHIQIGGGIrsldYAEKLRKlgyrRQIVSSkvle------dpsFLKSLrei--dvePVFSLDtrggrvafk-- 136 Thermotoga maritima
2AGK_A       70 EALqespqFLQVGGGInd-tNCLEWLKwa-sKVIVTSwlftke-ghfqlkRLERLtelcgkdrIVVDLScrktqdgrwiv 146 baker's yeast
CAA93307     74 TALhtwpgALQIGGGIni-sNAQNWLQegasKVIVTSwlfpda--qfdldRLKAIsskigkdrLVVDVScrrkdnrwfaa 150 fission yeast
CAG34734     70 SALaawpgGMQIGGGIny-dNACDWLDlgasHVIVTSwvfsng--qidegRLADLvrrvgkkrLVLDLScrrrgddyfiv 146 Desulfotalea psy...
BAD72794    117 GALhaypgGLQVGGGIns-eNCMSYIEegasHVIVTSyvfnng--kidleRLKDIvsivgkqrLILDLScrkkdgryaiv 193 thale cress
XP_568820    77 EAVsawrnGMQVGGGIte-tNAQEWLNngasKVIVTSylfpng--kfdedRLKRLanqagkdkLVVDIScrkrengwvva 153 Cryptococcus neo...
XP_956663    70 ESLkawpgGLQVGGGItd-aNAREWVEagaeKVIITSylfpngkfsqshlDAVLAaldgdkskLVIDLScrrqgddrwfv 148 Neurospora crass...
ZP_01077238  68 KALkaypnGLQFGGGVnh-kNASEFLKagasHLIVTSylfeng--efswdRLDRIkretgterLILDLScrrtatgwnia 144 Marinomonas sp. ...
EAT03275     69 EALaawpgGLQVGGGIta-aNAASWLDkgaaAVIVTSyvfrdg--lvledRLRELvkavgkqrLVLDLScrkrdgrylvv 145 delta proteobact...
CAL54280     85 EATrafpgGLQIGGGVnp-sNAKQFLDagasHVIVTSyvfrdg--mlnedALSEMvravgpdkLVLDLScrldasdgqyk 161 Ostreococcus tauri

Feature 1                                                                                       
1QO2_B      137 -----gwlaeeeidPVSLLKRLKeygleEIVHTEIEKDGtlqehdFSLTKKIAIea----eVKVLAAGGIssenSLKTAQ 207 Thermotoga maritima
2AGK_A      147 a-mnkwqtltdlelNADTFRELRky-tnEFLIHAADVEGlcggidELLVSKLFEwtkdyddLKIVYAGGAksvdDLKLVD 224 baker's yeast
CAA93307    151 i--nkwqvmtafelNEENLDLLSqy-csEFLIHAADVEGlckgidEDLVIKLGEwv----kIPTTYAGGGrsieDLDLVD 223 fission yeast
CAG34734    147 t--drwqkftevqvVPEVLERLAsc-cdEFLLHAADVEGkcegieRDLVLKLADws----pIATTYAGGIrgveDLQAIA 219 Desulfotalea psy...
BAD72794    194 t--drwqkfsdvilDEKSLEFLGgf-sdEFLVHGVDVEGkklgidEELVALLGNys----pIPVTYAGGVtvmdDVERIK 266 thale cress
XP_568820   154 m--ngwktltdmavTKESIQLVEry-csELLIHAADVEGlcqgidEELVTRLGEwv----sIPCTYAGGAkdisDLALVD 226 Cryptococcus neo...
XP_956663   149 a-mnkwqtitdmevSEESIKALEpy-csEFLIHAADNEGlqkgidEKLVQRLSEwc----sIPVTYAGGGrnleDLETVK 222 Neurospora crass...
ZP_01077238 145 t--drwqtitstqiNQENLNELAdh-cdEFLIHAADVEGlqagidTELVELLGKha----sIATTYAGGArtldDLALVQ 217 Marinomonas sp. ...
EAT03275    146 t--drwqkftemvvDGASLDYFSrf-caEFLIHAVDVEGkcagvaEDLLRLLGEqa----pLPTTYAGGVatmaDMHRID 218 delta proteobact...
CAL54280    162 vvtdrwqkwtdlavDSATLERLAkc-cdEFLVHGVDVEGmklgidLKLVELLGAfc----pIPVTYAGGArsleDLELVK 236 Ostreococcus tauri

Feature 1                                         
1QO2_B      208 KvhtetngllKGVIVGRAFLegi---ltVEVXKR 238 Thermotoga maritima
2AGK_A      225 Elsh----gkVDLTFGSSLDifggnlvkFEDCCR 254 baker's yeast
CAA93307    224 Klsk----gkVDLTIGSALDifgg-vleFTRVVA 252 fission yeast
CAG34734    220 Elgr----grIDATIGSALDifggslisYAEAVE 249 Desulfotalea psychrophila LSv54
BAD72794    267 Dagk----grVDVTVGSALDifgg-nlpYKDVVA 295 thale cress
XP_568820   227 Rlsn----gkVDLTFGSSLDifggkgvkFTDLVK 256 Cryptococcus neoformans var. neoformans JEC21
XP_956663   223 Rlsg----gkVDLTIGSALDcfggkgvtLQECVE 252 Neurospora crassa OR74A
ZP_01077238 218 Slsq----gkVDLTIGSALDifggegirLEQCIE 247 Marinomonas sp. MED121
EAT03275    219 Qlgg----grLDATIGSALDifggsgvkYRDAVA 248 delta proteobacterium MLMS-1
CAL54280    237 Asgr----gvVDITVGSALDcfgg-alkYDDVVA 265 Ostreococcus tauri

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