2BGG,1YTU,1W9H,1YTU,1YVU,1Z25,1Z26,1U04


Conserved Protein Domain Family
Piwi_piwi-like_ProArk
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cd04659: Piwi_piwi-like_ProArk 
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Piwi_piwi-like_ProArk: PIWI domain, Piwi-like subfamily found in Archaea and Bacteria. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The central component of the RNA-induced silencing complex (RISC) and related complexes is Argonaute. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of which provides the 5' anchoring of the guide RNA and the other, the catalytic site for slicing. This domain is also found in closely related proteins, including the Piwi subfamily, where it is believed to perform a crucial role in germline cells, via a similar mechanism.
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Statistics
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PSSM-Id: 240017
Aligned: 36 rows
Threshold Bit Score: 209.932
Created: 3-Oct-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active site5' RNA guide
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:Based on the structure of Argonaute and comparison to the RNase H active site.
  • Structure:1YVU_A; Aquifex aeolicus Argonaute protein binds divalent ion Ca++; defined at 3.5A contacts.
  • Structure:1Z25_A; Pyrococcus furiosus Argonaute protein binds divalent ion Mn2+; defined at 3.5A contacts.
  • Comment:Some members of this subfamily lack the canonical catalytic residues and are most likely unable to cleave the nucleic acid chain.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
2BGG_B     11 LTYRIGNGASVPISNTgelik--------------glrnyGPYEVPSLk--------yNQIALIHNNqfs-----slINQ 63  Archaeoglobus fulg...
1YVU_A    320 DTVLDAKGKNTKVITNlrkf----------------lelcRPFVKKDV----------LSVEIISVSvykk--lewrKEE 371 Aquifex aeolicus VF5
1Z25_A    333 IRVRDDKGNSVPISQLnvqksqlllw----tnysrkypviLPYEVPEKfrk----ireIPMFIILDSgllad-iqnfATN 403 Pyrococcus furiosus
1Z26_A    333 IRVRDDKGNSVPISQLnvqksqlllw----tnysrkypviLPYEVPEKfrk----ireIPMFIILDSgllad-iqnfATN 403 Pyrococcus furiosus
1U04_A    333 IRVRDDKGNSVPISQLnvqksqlllw----tnysrkypviLPYEVPEKfrk----ireIPXFIILDSgllad-iqnfATN 403 Pyrococcus furiosu...
BAB67041   13 VYYRMGSSGTNLVDSRyyi------------------irfGPYSKASPtdpninvdhyKKVLIICNRkdkrl-cdnlKND 73  Sulfolobus tokodai...
37999509  312 LLVKDVNKNPTKIYSTntlftwiy---------nqnakiyLPYDVPEI----------IRNKNLLTYili----deeIKD 368 Methanocaldococcus...
CAI09102  339 FRLLFRGAAVADSERAvlrhgayq---------gmtrklvVPLVVGGTal-------eQAVAVRHFEa--------vERV 394 Azoarcus sp. EbN1
YP_307281 343 PDLAFGNNTVFSVRGTpgttyvsleqlgqtrisalfnqkiGPYDSRPLd---------RQYMILPKSvwds--hgpvFLN 411 Dehalococcoides sp...
BAD71864  297 IGRRLGLGTPEAVRAQa-----------------------YRLSIPKLmgrra-vskpADALRVGFYraqetalallRLD 352 Thermus thermophil...

Feature 1                                                                                     
2BGG_B     64 LKSQISSKIdevwh---------------------ihninISEFIYDsph------fdSIKSQVDNAidt----gvDGIM 112 Archaeoglobus fulg...
1YVU_A    372 FLKELINFLknkgi----------------------klkiKGKSLILaqtr---eeakEKLIPVINKik-----dvDLVI 421 Aquifex aeolicus VF5
1Z25_A    404 EFRELVKSMyyslakkynslakkarstneiglpfldfrgkEKVITEDlns------dkGIIEVVEQVssfmkgkelGLAF 477 Pyrococcus furiosus
1Z26_A    404 EFRELVKSMyyslakkynslakkarstneiglpfldfrgkEKVITEDlns------dkGIIEVVEQVssfmkgkelGLAF 477 Pyrococcus furiosus
1U04_A    404 EFRELVKSXyyslakkynslakkarstneiglpfldfrgkEKVITEDlns------dkGIIEVVEQVssfxkgkelGLAF 477 Pyrococcus furiosu...
BAB67041   74 LEKGLPNNLifkpfne-----------------ifgtkldFQVVEYDsdr-----ydeEKIMKVYEDeye----prTFPL 127 Sulfolobus tokodai...
37999509  369 ELKAIKDKVnkmfrnynkian-------ktelpkfnyanrWKYFSTDdirg---iikeIKSEFNDEIc--------FALI 430 Methanocaldococcus...
CAI09102  395 CRQWHSDLPsw----------------------------kTAPPAGDae-------alNQRLAQRKPe--------NALL 431 Azoarcus sp. EbN1
YP_307281 412 DFKKIMNELylhel----------------------pynpIVVTYNDlsaktyalqgrAILDAVDSElre---pgyGVVM 466 Dehalococcoides sp...
BAD71864  353 GAQGWPEFLrrallr-------------------afgasgASLRLHTlhahp--sqglAFREALRKAkee----gvQAVL 407 Thermus thermophil...

Feature 1                                                                                     
2BGG_B    113 LVLPEyn-------tPLYYKLKSYLIns-----------------iPSQFMRYDILsnr---------nlTFYVDNLLVQ 159 Archaeoglobus fulg...
1YVU_A    422 VFLEEypkvdpyksfLLYDFVKRELLkk----------------xiPSQVILNRTLkne---------nlKFVLLNVAEQ 476 Aquifex aeolicus VF5
1Z25_A    478 IAARNkl------ssEKFEEIKRRLFnl----------------nvISQVVNEDTLknkrdky-drnrldLFVRHNLLFQ 534 Pyrococcus furiosus
1Z26_A    478 IAARNkl------ssEKFEEIKRRLFnl----------------nvISQVVNEDTLknkrdky-drnrldLFVRHNLLFQ 534 Pyrococcus furiosus
1U04_A    478 IAARNkl------ssEKFEEIKRRLFnl----------------nvISQVVNEDTLknkrdky-drnrldLFVRHNLLFQ 534 Pyrococcus furiosu...
BAB67041  128 VITPKlaks---qidSIYYRVKASFLdsgle-----------eratPTQIVTVDLLkqrden---kntnySWSLLPIAVQ 190 Sulfolobus tokodai...
37999509  431 IGKEKyk------dnDYYEILKKQLFdl----------------kiISQNILWENWrkd---------dkGYMTNNLLIQ 479 Methanocaldococcus...
CAI09102  432 LIGLGrga----dkrKIRNVAYRYGL--------------------ATQFMRLDHPprt---------yqSTYYNNLAAG 478 Azoarcus sp. EbN1
YP_307281 467 IHETVdr------rnRQHDQLAAMVMrelrnrrlyvsvihttvtkdCYQLPQNAPIgkaycpvagkqgklNGYLRNVAIT 540 Dehalococcoides sp...
BAD71864  408 VLTPPma-------wEDRNRLKALLLre----------------glPSQILNVPLRee-----------eRHRWENALLG 453 Thermus thermophil...

Feature 1                                    # #                                              
2BGG_B    160 FVSKL-GGKPWILNvdp----ekGSDIIIGTGATridn----vnlfCFAMVFKKD----GTMLWNEISPIvtsseyltyl 226 Archaeoglobus fulg...
1YVU_A    477 VLAKT-GNIPYKLKei-----egKVDAFVGIDISritrdgktvnavAFTKIFNSK----GELVRYYLTSYpafg--eklt 544 Aquifex aeolicus VF5
1Z25_A    535 VLSKL-GVKYYVLDyr------fNYDYIIGIDVApmkrs--egyigGSAVMFDSQ----GYIRKIVPIKIgeqrgesvdm 601 Pyrococcus furiosus
1Z26_A    535 VLSKL-GVKYYVLDyr------fNYDYIIGIDVApmkrs--egyigGSAVMFDSQ----GYIRKIVPIKIgeqrgesvdm 601 Pyrococcus furiosus
1U04_A    535 VLSKL-GVKYYVLDyr------fNYDYIIGIDVApxkrs--egyigGSAVXFDSQ----GYIRKIVPIKIgeqrgesvdx 601 Pyrococcus furiosu...
BAB67041  191 MFTKM-GGIPYALKqscinigseFNVHFIGLGLTsdprn--klkrvGFVTIFNDNg---SLSYMDSNILEdnkt---esy 261 Sulfolobus tokodai...
37999509  480 IMGKL-GIKYFILDsk------tPYDYIMGLDTGlgifg--nhrvgGCTVVYDSE----GKIRRIQPIETpap-----ge 541 Methanocaldococcus...
CAI09102  479 VFSKG-GGVLCAIDdm-----pgETDLFIGLDLGgvsq-----rapGLAFLFTREgaqlGWQLAEAQRGErved---avl 544 Azoarcus sp. EbN1
YP_307281 541 KVLLTnERWPFVIStp------lHADFTVAFDVQln--------taCFTFIGKSGsd-iRTVLKTSNQKErlsk---aqv 602 Dehalococcoides sp...
BAD71864  454 LLAKA-GLQVVALSga------yPAELAVGFDAGgresf--rfggaACAVGGDGGh--lLWTLPEAQAGEripq---evv 519 Thermus thermophil...

Feature 1                                   #                                                 
2BGG_B    227 KSTIKKVVYGFKKSNpd---wdVEKLTLHVSGKrpkmkDGETKILKETVEELkkqemvsrdVKYAILHLNEt----HPFW 299 Archaeoglobus fulg...
1YVU_A    545 EKAIGDVFSLLEKLGfk----kGSKIVVHRDGRl---yRDEVAAFKKYGELYg--------YSLELLEIIKrn--nPRFF 607 Aquifex aeolicus VF5
1Z25_A    602 NEFFKEMVDKFKEFNik---ldNKKILLLRDGRi---tNNEEEGLKYISEMFd--------IEVVTMDVIKnh--pVRAF 665 Pyrococcus furiosus
1Z26_A    602 NEFFKEMVDKFKEFNik---ldNKKILLLRDGRi---tNNEEEGLKYISEMFd--------IEVVTMDVIKnh--pVRAF 665 Pyrococcus furiosus
1U04_A    602 NEFFKEXVDKFKEFNik---ldNKKILLLRDGRi---tNNEEEGLKYISEXFd--------IEVVTXDVIKnh--pVRAF 665 Pyrococcus furiosu...
BAB67041  262 GRIIYNAINRIVNLSntnkssnYNILVVHYSGKsl--sKDDDQLIRNAIQIAl--------SKKRNIEVYVakvskSNIF 331 Sulfolobus tokodai...
37999509  542 RLHLPYVIEYLENKAnid--meNKNILFLRDGFi---qNSERNDLKEISKELn--------SNIEVISIRKn----NKYK 604 Methanocaldococcus...
CAI09102  545 GDLLERSLQAYRQVHlg---alPRRIALHRDGRl----FESLDVIRNFERDYs--------VRVDVLEVVKsg--cPPLY 607 Azoarcus sp. EbN1
YP_307281 603 RQTLLEVLRQEVGFGrr----tMQTIVVQRDGKl---fASEIAGAKDAIEIVkkegilpsdVSLNFIEIPKssvapFRLF 675 Dehalococcoides sp...
BAD71864  520 WDLLEETLWAFRRKAgr----lPSRVLLLRDGRv---pQDEFALALEALAREg--------IAYDLVSVRKsg--gGRVY 582 Thermus thermophil...

Feature 1                                                                                     
2BGG_B    300 VMgdpnnr--------fhpyeGTKVKLss-kRYLLTLLQpylkrn--glemvTPIKPLS--VEIVSDnwtse-eyyhNVH 365 Archaeoglobus fulg...
1YVU_A    608 SNekf--------------ikGYFYKLse-dSVILATYNqv---------yeGTHQPIK--VRKVYGe--------lPVE 653 Aquifex aeolicus VF5
1Z25_A    666 ANm------------------KMYFNLg--gAIYLIPHKlkq--------akGTPIPIK--LAKKRIikngkvekqsITR 715 Pyrococcus furiosus
1Z26_A    666 ANm------------------KMYFNLg--gAIYLIPHKlkq--------akGTPIPIK--LAKKRIikngkvekqsITR 715 Pyrococcus furiosus
1U04_A    666 ANx------------------KXYFNLg--gAIYLIPHKlkq--------akGTPIPIK--LAKKRIikngkvekqsITR 715 Pyrococcus furiosu...
BAB67041  332 LIdtdskttdkqgsltyhpriGIVLKLk--dNLYLMNMGgaitdrkinlptgGFPSTLLisIHKELSsge-----slDDD 404 Sulfolobus tokodai...
37999509  605 VFtsd-------------yriGSVFGN----DGIFLPHKtp---------fgSNPVKLStwLRFNCGnee----glkINE 654 Methanocaldococcus...
CAI09102  608 RRgvvaenkk----afrnpevGDAFELpgldELIIATYSgeelgs--swgdkVTVRPLR--LRKRYGe--------tDLH 671 Azoarcus sp. EbN1
YP_307281 676 DSsprpgqpe----mannpriGSYFIAtn-yDGYICTTGkef-------yhpGTANPLH--VKYIEGn--------mPFE 733 Dehalococcoides sp...
BAD71864  583 PVqgr-------------ladGLYVPLedktFLLLTVHRdf----------rGTPRPLK--LVHEAGd--------tPLE 629 Thermus thermophil...

Feature 1                                      #          
2BGG_B    366 EILDEIYYLSKMNWRGfr----srNLPVTVNYPKLVAGIIANVN 405 Archaeoglobus fulgidus
1YVU_A    654 VLCSQILSLTLXNYSSfq----piKLPATVHYSDKITKLXLRGI 693 Aquifex aeolicus VF5
1Z25_A    716 QDVLDIFILTRLNYGSisa---dmRLPAPVHYAHKFANAIRNEW 756 Pyrococcus furiosus
1Z26_A    716 QDVLDIFILTRLNYGSisa---dmRLPAPVHYAHKFANAIRNEW 756 Pyrococcus furiosus
1U04_A    716 QDVLDIFILTRLNYGSisa---dxRLPAPVHYAHKFANAIRNEW 756 Pyrococcus furiosus DSM 3638
BAB67041  405 DLVKSVFIMSRVSYSNlsn--pvlSEPITIKYSRSIAYLTLRLS 446 Sulfolobus tokodaii str. 7
37999509  655 SIMQLLYDLTKMNYSAlygegrylRIPAPIHYADKFVKALGKNW 698 Methanocaldococcus jannaschii
CAI09102  672 TLARQVVLLSRIHGASlyr---hpRLPVTTHHADRFATLRQECN 712 Azoarcus sp. EbN1
YP_307281 734 KILEDVYALTCLALTRped---ctREPFTMKLADIRLREHAGGY 774 Dehalococcoides sp. CBDB1
BAD71864  630 ALAHQIFHLTRLYPASgfa---fpRLPAPLHLADRLVKEVGRLG 670 Thermus thermophilus HB8

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