Conserved Protein Domain Family
AAK_P5CS_ProBA
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cd04256: AAK_P5CS_ProBA 
AAK_P5CS_ProBA: Glutamate-5-kinase (G5K) domain of the bifunctional delta 1-pyrroline-5-carboxylate synthetase (P5CS), composed of an N-terminal G5K (ProB) and a C-terminal glutamyl 5- phosphate reductase (G5PR, ProA), the first and second enzyme catalyzing proline (and, in mammals, ornithine) biosynthesis. G5K transfers the terminal phosphoryl group of ATP to the gamma-carboxyl group of glutamate, and is subject to feedback allosteric inhibition by proline or ornithine. In plants, proline plays an important role as an osmoprotectant and, in mammals, ornithine biosynthesis is crucial for proper ammonia detoxification, since a G5K mutation has been shown to cause human hyperammonaemia.
Links
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Statistics
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PSSM-Id: 239789
Aligned: 11 rows
Threshold Bit Score: 437.634
Created: 29-Sep-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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Feature 1:putative nucleotide binding site [chemical binding site]
Evidence:
  • Comment:Based on similarity to Campylobacter jejuni glutamate 5-kinase
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                      #                                                              
6226882    63 HRSELKHAKRIVVKLGSAVVTRGDecgLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQAlh 142 human
AAU90213    7 SRSFVRDVKRVIIKVGTAVVSRQDg-rLALGRVGALCEQVKELNSLGYEVILVTSGAVGVGRQRLRYRKLVNSSFADLqk 85  Japanese rice
XP_781524  19 SRAELKSSKRIVLKLGSAVITRDDecgLALGRLASIVEQVSELQSSGRQVVLVTSGAVAFGKQRLRHEVMLSKSIKQTla 98  purple urchin
AAF51799   49 ERSQLKYARRLVVKLGSAVITREDnhgLALGRLASIVEQVAECHLEGREVMMVTSGAVAFGKQKLAQELLMSLSMRETln 128 fruit fly
AAM48244  126 YRHQLSHAKRIVIKMGSAVITREDgngLALGRLAAIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETis 205 Tigriopus californ...
1709536    72 TRNDLKKAQRVVVKLGSAVITREDecgLALGRLASIVEQVSELQQSGRQMLIVSSGAVAFGRQKLRQELVMSMSMRQTlr 151 Caenorhabditis ele...
XP_421834  68 HRSELKHAKRIVVKLGSAVVTRGDecgLALGRLASIVEQVSMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQAlh 147 chicken
AAH74114   64 HRSELKHAKRIVVKLGSAVVTRGDecgLALGRLASIVEQVSVLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQAlh 143 African clawed frog
XP_706856  43 HRSELRQAKRIVVKLGSAVVTRGDecgLALGRLASIVEQVAMLQNQGREMMIVTSGAVAFGKQRLRHEILLSQSVRQAlh 122 zebrafish
NP_191120   7 SRAFAKDVKRIVVKVGTAVVTGKGg-rLALGRLGAICEQLAELNSDGFEVILVSSGAVGLGRQRLRYRQLVNSSFADLqk 85  thale cress
AAB67875    7 TRAFVKNVKRLIVKVGTAVVTRADg-rLALGRLGALCEQLQELNSQGYEVILVTSGAVGVGRQRLRYRKLLNSSFLDLqk 85  Lycopersicon escul...

Feature 1                                                                                     
6226882   143 sgqn--qlkemaIPVLEARACAAAGQSGLMALYEAMFTQYsICAAQILVTNLDFHDEqKRRNLNGTLHELLRMNIVPIVN 220 human
AAU90213   86 -----------pQMELDGKACAAVGQSGLMALYDMLFNQLdVSSSQLLVTDSDFENPkFREQLTETVESLLDLKVIPIFN 154 Japanese rice
XP_781524  99 pnss--klrrtsIIQMDSRACAAAGQSGLMSLYEAMFSQYgLTTAQVLVTKPDFYHDyTRSNLRGTMQELLRMNIIPIIN 176 purple urchin
AAF51799  129 pkd----skefdGATLEPRAAAAVGQSGLMSLYDAMFAQYgVKIAQVLVTKPDFYNEeTRNNLFCTLSELISLNIVPIIN 204 fruit fly
AAM48244  206 gnyntkediktlMKSSFKKPNAAVGQSGLQALYETMFRNYgILVGQVLVTKPDFYNDnTRQQLFTTINELLQLNIIPIIN 285 Tigriopus californ...
1709536   152 g---------psGMTADKRACAASGMPGLMSLYEQLFQQYgITVAQVLLTKPDIDDDqRRKNLQATIESLLSLNIIPIVN 222 Caenorhabditis ele...
XP_421834 148 sgqs--qlkdmaIPVLEARACAAAGQSGLMALYEAMFTQYsICAAQILVTNLDFHDEqKRRNLNGTLHELLRMNIVPIIN 225 chicken
AAH74114  144 sgqn--qlkdmqVPVLEARACAAAGQSGLMALYEAMFTQYsICAAQILVTNLDFHDEqKRRNLNGTLHELLRMNIVPIIN 221 African clawed frog
XP_706856 123 sgqn--qlkdmsLPVLEARACAAAGQSGLMALYEAMFTQYsTCTAQILVTNLDFHDEqKRRNLNSTLYELLRMNIVPIIN 200 zebrafish
NP_191120  86 -----------pQMELDGKACAGVGQSSLMAYYETMFDQLdVTVAQMLVTDSSFRDKdFRKQLSETVKAMLRMRVIPVFN 154 thale cress
AAB67875   86 -----------pQTELDGKACAAVGQNGLMALYDSLFSQLdVTSAQLLVTDNDFRDPdFRRQLNDTVNSLLSLKVIPIFN 154 Lycopersicon escul...

Feature 1                                                     ###  ##                         
6226882   221 TNDAVVPpae-pnSDLQGVnvi--svkDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGsDDAKLIDIFYp-gDQQSVT 296 human
AAU90213  155 ENDAISTrka-pyEDSSGIf------wDNDSLAGLLALELKADLLILLSDVDGLYSGPPSePSSKIIHTYIkekHQQEIT 227 Japanese rice
XP_781524 177 SNDAVVPppe-edKDLAGVis----ikDNDSLAARLAIEINADLLVLLSDVDGIFSAPPGqEGASFLDTVYp-gDTAFIE 250 purple urchin
AAF51799  205 TNDAVSPpmfirdDEPAGGarrgipikDNDSLSAMLAAEVQADLLILMSDVDGIYNKPPWeDGAKLMHTYTs-dDSNSIE 283 fruit fly
AAM48244  286 TNDAVSPppq-kdEDPEGVlg----ikDNDSLAARVAVETRADLAILMSDVDGIYDRSPShEDARVMHNFNp-vDLAKVE 359 Tigriopus californ...
1709536   223 ANDAVAPd-----PKLNMHi------sDNDSLAARLSAEIEAELLIILSNVNGVYTGPPDlEGSRLLYTYVp-sENSGVT 290 Caenorhabditis ele...
XP_421834 226 TNDAVVPppe-pnSDLQGVis----vkDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGsDDAKLIDIFYp-gDQQSVT 299 chicken
AAH74114  222 TNDAVVPppe-pnSDLQGVis----ikDNDSLAARLAVEMKADLIIVLSDVEGLFNSPPGsDDAKLIDIFYp-gDQQSVT 295 African clawed frog
XP_706856 201 TNDAVVPppe-pnSDLQGVis----ikDNDSLAARLAVEMRADLLIALSDVEGLYDSPPGsDDAKLLDTFYp-vDHQSIT 274 zebrafish
NP_191120 155 ENDAISTrra-pyKDSTGIf------wDNDSLAALLSLELKADLLILLSDVEGLYTGPPSdSTSKLIHTFIkekHQDEIT 227 thale cress
AAB67875  155 ENDAISTrra-pyEDSSGIf------wDNDSLAALLALELKADLLVLLSDVDGLYSGPPRdPDSKLIYTYIkeiHERVIT 227 Lycopersicon escul...

Feature 1             # #                      #                                           
6226882   297 FGTKSRVGMGGM-------------------EAKVKAALWALQGGTSVVIANGthpkvsghVITDIVEGKKVGTFFS 354 human
AAU90213  228 FGDKSRVGRGGM-------------------TAKVKAAVLASNSGTPVVITSGfe----nrSILKVLHGEKIGTLFH 281 Japanese rice
XP_781524 251 FKGTSRVGLGGM-------------------ESKVKAANWALERGCSVVIGNGmnr---ddIVLNILNGRKVGTFFT 305 purple urchin
AAF51799  284 FGKKSKVGTGGM-------------------DSKVKAATWALDRGVSVVICNGmq----ekAIKTIIGGRKVGTFFT 337 fruit fly
AAM48244  360 FGEKSDAGTGGM-------------------ESKVRSALWALENGSSVVICNGmk----ynTIRKIMRGDKVGSFFT 413 Tigriopus californicus
1709536   291 FGANSKFGTGGM-------------------ESKVTACVNALNNGVTTVITNGla----qdAITDAVAGKKIGTMFC 344 Caenorhabditis elegans
XP_421834 300 FGTKSRVGMGGM-------------------EAKVKAALWALQGGTSVVIANGthpkisghVITDIVEGKKVGTFFS 357 chicken
AAH74114  296 FGTKSRVGMGGMeakpykarssalcssistpPFSVKAALWALQGGTSVVIANGtypkisghVITDIVEGKKVGTFFS 372 African clawed frog
XP_706856 275 YGTKSRVGIGGM-------------------EAKVKAALWALQGGTSVVIANGthpkvtghVITDIVEGKKVGTFFS 332 zebrafish
NP_191120 228 FGEKSKLGRGGM-------------------TAKVKAAVNAAYGGVPVIITSGya----aeNISKVLRGLRVGTLFH 281 thale cress
AAB67875  228 FGDKSRVGRGGM-------------------TAKVKAAMYAAYAGIPVVITSGfa----tdNIIKVLHGERIGTLFH 281 Lycopersicon esculentum

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