1GA8


Conserved Protein Domain Family
GT8_A4GalT_like

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cd04194: GT8_A4GalT_like 
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A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface.
The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS.
Statistics
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PSSM-Id: 133037
View PSSM: cd04194
Aligned: 88 rows
Threshold Bit Score: 150.828
Threshold Setting Gi: 42518148
Created: 31-Oct-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Ligand bindingmetal binding
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:Ligand binding site
Evidence:
  • Structure:1GA8_A: Neisseria Lgtc with bound Mn and donor sugar analog UDP-2-deoxy-2-fluorogalactose
    View structure with Cn3D
  • Structure:1GA8_A: Neisseria Lgtc with bound sugar acceptor analog 4'-deoxylactose
    View structure with Cn3D
  • Comment:Donor and acceptor binding seems to be cooperative; in some glycosyltransferases the nucleotide-sugar bind first, and in others, the acceptor

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            ### ##                                                                     
1GA8_A        1 XDIVFAADdNYAAYLCVAAKSVEAAHpd--------------------tEIRFHVLDAg--iSEANRAAVAAnlrg---- 54  Neisseria mening...
AAD07221      5 IPIVIAFDnHYAMPAGVSLYSMLACAktehpqs---------qndseklFYKIHCLVDn--lSLENQSKLKEtlapf--s 71  Helicobacter pyl...
AAD08459     11 IPIFMSFDkNYALGASVSLYSLLSHAsrhtsmidfsplnqsnkllganiVYKIHCLVKg--vTLEQQNKLLKtiapf--k 86  Helicobacter pyl...
YP_385284    10 IPVFFAFDnNYVIPAAVAFHSLLANVnv-------------------syKYHFIVLHEd--iSEENRDLLAQvvslf--- 65  Geobacter metall...
YP_170195     4 IPIVFTFDkNIILGGAVTIKSLIDHAnp-------------------dtCYDIYVYHPn--iNKKSISAFNSmiek---- 58  Francisella tula...
YP_967832     4 VPIVFTFDaNYRLPASVALQSLFENAkd-------------------stYYHVYLVCEg--lSRGDKDAIESicpe---- 58  Desulfovibrio vu...
AAO77979      6 VPLVIAFTpDYFIPAATCLYSIITSMqa-------------------egELHVICLLSee-lPERLKLKIQLige----- 60  Bacteroides thet...
ZP_00371132   2 LHICIGVSaEYVKYSAVLINSIVKATqkpfdlkpyen--nlsftkdlkeGFCFHIFTEykseDTEKIALLAHklsei--- 76  Campylobacter up...
AAV66459      9 FHIVLNANeNYIKYAAVLMTSIIQKTdlnksmse-------fcnfdtdeGYVFHILSDh--iSESMKVRISNlekql--n 77  Campylobacter je...
CAI38726      2 YHIVFNSSnEYIENLSVLMYSIIINTnks-----------------ntkKYCFHILSSn--iNDNTCKKLTLlekelssi 62  Campylobacter je...
Feature 1                                                     #  #                 ###          
1GA8_A       55 gggnIRFIDVNPEdfagfpl------------------nirhisITTYARLKLGEYia-dcDKVLYLDIDVLVRDSLTPL 115 Neisseria mening...
AAD07221     72 afssLEFLDISTPnlhatpiepsaidkineaflqlniyaktrfsKMVMCRLFLASLfp-qyDKIIMFDADTLFLNDVSES 150 Helicobacter pyl...
AAD08459     87 kfasLEFIHINSLdhsiesyln-------------kscskryggLLVLCRLLLASLfp-nySKIISIDADTVFLGDVASA 152 Helicobacter pyl...
YP_385284    66 snasVEFRDMGESfkneweni----------------kgkghytKECLYKLVPMLEfp-qyDKIIWSDVDVVFKDDISDV 128 Geobacter metall...
YP_170195    59 tkhsISFHNVDESifkdvpi------------------dtrrgwIITFYRLLIPKLlp-qyDKVIYSDVDVLFQSDMSEV 119 Francisella tula...
YP_967832    59 kngrVEWIDVDNElfssap-------------------ssenwpKIVYARILLPLLlp--fDKVIYSDVDVVFCSDLAEI 117 Desulfovibrio vu...
AAO77979     61 --grTCYSFVNLQgklqhiy------------------idqkytEAASYRLLLPDLlp-eyKKVIYIDCDIIVRNDLVQL 119 Bacteroides thet...
ZP_00371132  77 yptkCLIHTMNNQdfqdfsy------------------pfwcqnAAMFYKIKVADIlk-dvDKCLFIGADLFALGDVRDL 137 Campylobacter up...
AAV66459     78 diypCKIVLHILNddefkgm------------------lkwrgnYLAYYRIKMASVlpqnlKICLYLDCDMLCFGDLREL 139 Campylobacter je...
CAI38726     63 ypseIKIYHINDNlfydyni------------------pkhegsYNAYLRLMLASIlskdiKKCLYLDVDMLVLGDISEL 124 Campylobacter je...
Feature 1                       #                                          ###                  
1GA8_A      116 Wdtdl--gdnWLGASIDlfverqegyk--------------------qkigxadgeyYFNAGVLLINLKkWRRHDIFKXS 173 Neisseria mening...
AAD07221    151 Ffipl--dgyYFGAAKDfasdkspkhfqivrekdprqafslyehylnesdmqiiyesNYNAGFLVVNLKlWRADHLEERL 228 Helicobacter pyl...
AAD08459    153 YfaldndptkSLGMVRDtfshlsfeafcdfckrack--nfkidfsrfspdelkrihqGFNMGFLVAHLDrWRQDGFEKTA 230 Helicobacter pyl...
YP_385284   129 Ffmls--eenYIAGVRVcgkldkyyenmn-----------------mpaeiksilknGIGAGILVYNLKkMREDNIYDDI 189 Geobacter metall...
YP_170195   120 Yntdl-tsyeWAGVIAEkhqqnmvqhk---------------------yfkennnsyIYWPGFMVMNTKlMRENNFISRC 177 Francisella tula...
YP_967832   118 Fqiev-dgceWAGVAAElvafqegvarc------------------hnvhceyqnelIYMSGFMVMNLRlMREKDTVGRC 178 Desulfovibrio vu...
AAO77979    120 Yhsid-lgmnYLAAVFEasmdfqldhl---------------------ktigcnpneYINSGFLIMNLElMRKDNMVEKF 177 Bacteroides thet...
ZP_00371132 138 Faldl--kdnLIAAALDtynfdgylrkakak-------------nsdeelvfndaknYINNDMMLINLKeWRKQNLQAKY 202 Campylobacter up...
AAV66459    140 Lsvdi--nnyQAAVCLDgnnhkknkkvffsl-------------kgrekykfsniekYFNSGFILVNLDrWRRDNIENKS 204 Campylobacter je...
CAI38726    125 Fdldl--kdkVFAAVFIlkhpwpnlnsk------------------dsseifyiygsHFNSGLMLINLDaWREKNIESRS 184 Campylobacter je...
Feature 1                     ##                      #                                         
1GA8_A      174 SEWVeqykdvxqyqDQDILNGLFKGg-VCYANSRFNFXPTNyafxanwfasrht---------------------dplyr 231 Neisseria mening...
AAD07221    229 LNLThqkgqcvfypEQDLLTLACYQk-VLILPYIYNTHPFManqkrf--------------------------------- 274 Helicobacter pyl...
AAD08459    231 LEFLktrgkdlfypEQCLVNMVFWEr-ILELPIYYNCYSDFfkeh----------------------------------- 274 Helicobacter pyl...
YP_385284   190 MIALqgmssivvqpEQDILNIVLKDk-IDYIPLRYCFCTYMynlfkdrhkmklkvkgnlfnylfkgyrknlgfdtiysek 268 Geobacter metall...
YP_170195   178 FDTMhefntrlkfrDLDVLNLTCRK--IKSLPFKYVTLQSIyylntiqeapeyifl-----------------keiysdn 238 Francisella tula...
YP_967832   179 LNNIskfgsrlkmyDLEILNMSSDN--IARIDFSYCVLENVffaknvseakeypwl-----------------rglyrvs 239 Desulfovibrio vu...
AAO77979    178 IEASkvdy--lefpDQDVLNQLCKDr-ILALPPYYNSIRTFylpqykkfflqk------------------------yte 230 Bacteroides thet...
ZP_00371132 203 IDYLnkyd---lagDLDVFPLVCAPk-IHILSSKYNFILGYytresfglentlkdesd-------------kpvwnftkv 265 Campylobacter up...
AAV66459    205 IDFLkkfk--tlypDQDALNFALND--TLLLPNRWNFSLGYfvaflknsqeilflnqtk------------yphlnytkt 268 Campylobacter je...
CAI38726    185 LSFIknyyv-pyavDEYVLNAILSKddIFSLKLEWNFLIGFrrlylnndlffnkeegdk------------ykiicyske 251 Campylobacter je...
Feature 1                   # ##    #    
1GA8_A      232 drtntvxpVAVSHYCGp--AKPWHR 254 Neisseria meningitidis
AAD07221    275 --ipdkkeIVMLHFYFv--GKPWVL 295 Helicobacter pylori 26695
AAD08459    275 ----ypknIIMLHFIK---YKPWRS 292 Helicobacter pylori 26695
YP_385284   269 elleafesPAIIHYATs--TKPWNT 291 Geobacter metallireducens GS-15
YP_170195   239 elldaknnPAIIHYAGs-pGKPWRM 262 Francisella tularensis subsp. tularensis SCHU S4
YP_967832   240 eleaarsaPRIIHFAGs-dTKVWER 263 Desulfovibrio vulgaris subsp. vulgaris DP4
AAO77979    231 qdwlevhrHGTVHYTG---AKPWNQ 252 Bacteroides thetaiotaomicron VPI-5482
ZP_00371132 266 eleqiqkdLRLVHFCHy-vYKPWMS 289 Campylobacter upsaliensis RM3195
AAV66459    269 efenevknIKIAHFILd-pFKPWDA 292 Campylobacter jejuni subsp. doylei
CAI38726    252 efekafkkIKILHYTYlymPKPWEN 276 Campylobacter jejuni

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