Conserved Protein Domain Family
Rab20

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cd04126: Rab20 
Rab GTPase family 20 (Rab20)
Rab20 is one of several Rab proteins that appear to be restricted in expression to the apical domain of murine polarized epithelial cells. It is expressed on the apical side of polarized kidney tubule and intestinal epithelial cells, and in non-polarized cells. It also localizes to vesico-tubular structures below the apical brush border of renal proximal tubule cells and in the apical region of duodenal epithelial cells. Rab20 has also been shown to colocalize with vacuolar H+-ATPases (V-ATPases) in mouse kidney cells, suggesting a role in the regulation of V-ATPase traffic in specific portions of the nephron. It was also shown to be one of several proteins whose expression is upregulated in human myelodysplastic syndrome (MDS) patients. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins.
Statistics
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PSSM-Id: 133326
View PSSM: cd04126
Aligned: 4 rows
Threshold Bit Score: 351.902
Threshold Setting Gi: 9965335
Created: 7-Feb-2006
Updated: 2-Oct-2020
Structure
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Aligned Rows:
  next features
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:Based on sequene similarity with other Rab isoforms.
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #######         #    ##                     #                           
NP_060287   6 SKIVLLGDMNVGKTSLLQRYMERRFPDTVSTVGGAFYLKQWrSYNISIWDTAGREQFHGLGSMYCRGAAAIILTYDVNHR 85  human
CAG05847   12 VKVVLLGDMNVGKTSLLHRYMERKFKETISTVGGAFYLKQWgPYNISIWDTAGREQFHGLGSMYCRGAAAVIFIYDVTNW 91  Tetraodon nigrovir...
AAH73712    6 LKVVLLGDMNVGKTSLLHRYMERRFQDTVSTVGGAFYLKQWgPYNISIWDTAGREQFHGLGSMYCRAASAVILTYDVSNM 85  African clawed frog
AAG10047   11 LKVIVMGDFNVGKTSFIGRYIEGEFKQHDATIGAAFFLKQWgPYNIAIWDTAGAERFTGLSSFYCKNAGAAILAFDMSIV 90  Pacific oyster
Feature 1                                ## #                                                 
NP_060287  86 QSLVELEDRFLGLTDTASKDCLFAIVGNKVDlteegalagqekeec---------------------spnmdagdrvspr 144 human
CAG05847   92 QSLAELEERFLSLTDTANHDCIYAVVGNKADltdpkaqqlqdpeaasetrtegplegsepqvfsacptppaspaslsglm 171 Tetraodon nigrovir...
AAH73712   86 QSLLELEDRFLGLTDTASDDCIFAVVGNKIDltedynsdsdie----------------------------gerprtssk 137 African clawed frog
AAG10047   91 STFESLWARFIPLLEVANEDCLKIVVGMKLDalg---------------------------------------------e 125 Pacific oyster
Feature 1                                           ###                                       
NP_060287 145 apKQVQLEDAVALykkilkykmldeqdvpaaeqMCFETSAKTGYNVDLLFETLFDLVVPMILqqraerpsh---tvdiss 221 human
CAG05847  172 lhKQVSPEDAKAFyeriirykglegksslpadkMCFETSAKSGYNVDALFEALFNLVLPFILrkrnenqvsptvdleecr 251 Tetraodon nigrovir...
AAH73712  138 irRQVNLEDAFALykrimkykmldenvvpaaekMCFETSAKTGYNVDVLFEGVFNMVIPLIVkkkasgld----etvnla 213 African clawed frog
AAG10047  126 dqREVTVQEGKKFareindki----dlsklpsdPYFETSSKTGHNVTEVFEYIFRYCLPLSEaqlqaanr-----qgtvn 196 Pacific oyster
Feature 1                 
NP_060287 222 hkPPKRTRSGCC 233 human
CAG05847  252 ggSSKRSKSSCC 263 Tetraodon nigroviridis
AAH73712  214 qsKPNKSKSRCC 225 African clawed frog
AAG10047  197 lpNGKQSQKKCC 208 Pacific oyster

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