1YZT


Conserved Protein Domain Family
Rab21

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cd04123: Rab21 
Click on image for an interactive view with Cn3D
Rab GTPase family 21 (Rab21)
The localization and function of Rab21 are not clearly defined, with conflicting data reported. Rab21 has been reported to localize in the ER in human intestinal epithelial cells, with partial colocalization with alpha-glucosidase, a late endosomal/lysosomal marker. More recently, Rab21 was shown to colocalize with and affect the morphology of early endosomes. In Dictyostelium, GTP-bound Rab21, together with two novel LIM domain proteins, LimF and ChLim, has been shown to regulate phagocytosis. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 133323
View PSSM: cd04123
Aligned: 21 rows
Threshold Bit Score: 254.456
Threshold Setting Gi: 74833848
Created: 11-Jan-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II
  • Structure:1YZT: Human Rab21 binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #  ####         #     ##                                                
1YZT_B     21 FKVVLLGEGCVGKTSLVLRYCENKFndkhITTLQASFLTKKLnig---------gkRVNLAIWDTAGQERFHALGPIYYR 91  human
AAX97474    3 YKVVLLGEGRVGKSSIGNKWQSNTFdpqrRSTIAAAFFQKTVicq---------gkNVDIQLWDTAGQEEYHSLAPIYYK 73  Trichomonas vaginalis
AAX97475    3 YKVVFLGEGRVGKTSIGKRWVDGFFdpsmRSTISAAFFQKTVnag---------skKINIQLWDTAGQEEYHSLAPIYYK 73  Trichomonas vaginalis
EAN77581    8 YKVVLLGEGRVGKTSLISRFVNDTFdaqqRSTTQASMYSSVNvpll------nsdkTVNLNVWDTAGQERFHALGPIYYR 81  Trypanosoma brucei
EAN88125    5 YKVVLLGEGRVGKTSLVSRFAHDAFdenqSSTIQASMYTSADvplld-----gsgaKVKINVWDTAGQERFHALGPIYYR 79  Trypanosoma cruzi
CAI39252   11 YKVVTLGEGRVGKTSIITRFFDNSFseqtQETSDGYCKEKTIqtk---------kgPIDLAVWDTAGQEKYHSLAPLYYR 81  Paramecium tetraur...
CAI39377   11 YKVVFLGENKVGKTSIITRYFYNTFsektEVTVCDFCKYKTIqtk---------ngSIELAVWDTAGQEKYHALAPIYYR 81  Paramecium tetraur...
CAJ04282   10 FKVVLLGEGRVGKTSLISRYVHNAFdekeASTVQASMHSSKAvpindassapgairEVDLALWDTAGQERFHALAPMYYR 89  Leishmania major
XP_812628   8 MKIVMLGEGRVGKTSLLQRFVSDSFeeecESTTKASMYANVTvtvs-------dgaKAKLSIWDTAGQERYHALGPIYYR 80  Trypanosoma cruzi ...
AAX97477    8 NKVVLVGTTHTGKTSIVQRYVNGQFminsVSSMQAAFFQKNVmig---------gkEQTLEIWDTAGQERFRSLTPMYYR 78  Trichomonas vaginalis
Feature 1                                               ## #                           ###    
1YZT_B     92 DSNGAILVYDITDEDSFQKVKNWVKELRKMLGn-eICLCIVGNKIDle-keRHVSIQEAESYAESVGAKHYHTSAKQNKG 169 human
AAX97474   74 DAQAALLVYSVIDSTSFERMMQWKNELVNTRGe-nIKLIIAANKIDmv-sqRVITPQQGLDFANKVGCQIFEVSAKTGEG 151 Trichomonas vaginalis
AAX97475   74 DAVAAILVYSVTDSKSFEKMQQWRKELVSSRGd-nIKIILCGNKIDlp-sqRTVQYDQAEAIAKQYGCPYFEVSAKTGQG 151 Trichomonas vaginalis
EAN77581   82 NANGAILVYDVTDADTLEKVRLWIRELRAVVGd-qIQLVVCANKSDle-qeREVSEEKGRRFAKDHGAQHLSTSARTGMN 159 Trypanosoma brucei
EAN88125   80 NADGALLVYDITDADTLEKVRLWIRELRAVVGn-qIQLVICGNKSDre-eeRDISKEKAEEFAKSHEALHFVTSAKTGEN 157 Trypanosoma cruzi
CAI39252   82 NSDAAIIVYDITVKETLQKGRQWIQELKQMAEndnMLLVIVGNKCDmh-iqKDVEQSQIDELCNQYNAKHFLVSAKSGKG 160 Paramecium tetraur...
CAI39377   82 KADAAIIVYNITVQQTFQNVRLWIQELQQFSQnanIIPVIVGNKDDlh-mhRAVEQSQVDELCNQYNAKHFQVSAKSDKG 160 Paramecium tetraur...
CAJ04282   90 NADGAIIVYDVTDADTLRKVRTWAKELYAVVGegkIQLVLCGNKADtplaeREVSEGEGAAMAAELGASHFFASAKTGQN 169 Leishmania major
XP_812628  81 DAQGAILVYDITDYESFLKVKIWLKELQQVVGnqnISVVVVGNKIDle-rqRSVTVKEAEEWANQQNAKHYNVSAKLSIH 159 Trypanosoma cruzi ...
AAX97477   79 DARVAIVVFDITDANSFSKAKQWINELKSSRGd-sISLVLVGNKSDle-fiRVVQMSEAKQYAKLLDIPYFETSAKTGVN 156 Trichomonas vaginalis
Feature 1                  
1YZT_B    170 IEELFLDLCKRMI 182 human
AAX97474  152 IDMLFQALAKTLL 164 Trichomonas vaginalis
AAX97475  152 IETMFLRLGEILS 164 Trichomonas vaginalis
EAN77581  160 VAEAFQTLATAIA 172 Trypanosoma brucei
EAN88125  158 VAVAFQALATAVA 170 Trypanosoma cruzi
CAI39252  161 IPEIFDHLANELK 173 Paramecium tetraurelia
CAI39377  161 ITEIFDYIADGKQ 173 Paramecium tetraurelia
CAJ04282  170 VAEVFSAMATQVA 182 Leishmania major
XP_812628 160 ISEPFNGIAKSVI 172 Trypanosoma cruzi strain CL Brener
AAX97477  157 IEEVFIQVAQDVT 169 Trichomonas vaginalis

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