2EW1


Conserved Protein Domain Family
Rab30

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cd04114: Rab30 
Click on image for an interactive view with Cn3D
Rab GTPase family 30 (Rab30)
Rab30 subfamily. Rab30 appears to be associated with the Golgi stack. It is expressed in a wide variety of tissue types and in humans maps to chromosome 11. GTPase activating proteins (GAPs) interact with GTP-bound Rab and accelerate the hydrolysis of GTP to GDP. Guanine nucleotide exchange factors (GEFs) interact with GDP-bound Rabs to promote the formation of the GTP-bound state. Rabs are further regulated by guanine nucleotide dissociation inhibitors (GDIs), which facilitate Rab recycling by masking C-terminal lipid binding and promoting cytosolic localization. Most Rab GTPases contain a lipid modification site at the C-terminus, with sequence motifs CC, CXC, or CCX. Lipid binding is essential for membrane attachment, a key feature of most Rab proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 133314
View PSSM: cd04114
Aligned: 6 rows
Threshold Bit Score: 338.41
Threshold Setting Gi: 17555898
Created: 9-Aug-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Comment:The active conformation of Rab is stabilized by interations between the gamma phosphate of GTP and two critically conserved residues, Thr in switch I and Gly in switch II
  • Structure:2EWI: Human Rab30 binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                       #######         #     ##                         #               
2EW1_A        20 MEDYDFLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEINGEKVKLQIWDTAGQERFRSITQSYYRSA 99  human
Q15771         3 MEDYDFLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEINGEKVKLQIWDTAGQERFRSITQSYYRSA 82  human
NP_001002750   1 MEDYDYLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEIKGVKVKLQIWDTAGQERFRSITQSYYRSA 80  zebrafish
NP_499328      1 MEDYKYLFKVVLVGNAGVGKTCLVRKFTQGIFPPGQSATIGVDFMIKTVKVGNDKIKLQIWDTAGQERFRSITQSYYRSA 80  Caenorhabditis ...
AAH72360       3 MEDYDFLFKIVLIGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEIKGEKIKLQIWDTAGQERFRSITQSYYRSA 82  African clawed ...
NP_723242      1 MEDYKFLFKIVLVGNAGVGKTCLVRRFTQGLFPPGQGATIGVDFMIKTVEVEGEKIKLQIWDTAGQERFRSITQSYYRSA 80  fruit fly
Feature 1                                               ## #                            ##       
2EW1_A       100 NALILTYDITCEESFRCLPEWLREIEQYASNKVITVLVGNKIDLaerREVSQQRAEEFSEAQd-mYYLETSAKESDNVEK 178 human
Q15771        83 NALILTYDITCEESFRCLPEWLREIEQYASNKVITVLVGNKIDLaerREVSQQRAEEFSEAQd-mYYLETSAKESDNVEK 161 human
NP_001002750  81 NALILTYDITCEDSFRCLPEWLREIEQYANNQVVTILVGNKIDLadkREVLRQRAEEFADSQs-mLYLETSAKESDNVEK 159 zebrafish
NP_499328     81 HAIVLVYDVSCQPSFDCLPEWLGEIESYANRRVLKILVGNKVDKgdeREVPERIGRDFSDVNqfdYFLETSALDATNVDQ 160 Caenorhabditis ...
AAH72360      83 NALILTYDITCEESFRCLPEWLREIEQYASSEVITVLVGNKIDLaerREVSQQRAEEFAGTQn-mYYLETSAKESDNVEK 161 African clawed ...
NP_723242     81 HALILVYDISCQPTFDCLPDWLREIQEYANSKVLKILVGNKTDRd-dREIPTQIGEEFAKQHd-mYFLETSAKEAENVER 158 fruit fly
Feature 1                  
2EW1_A       179 LFLDLACRLI 188 human
Q15771       162 LFLDLACRLI 171 human
NP_001002750 160 LFLDLACELI 169 zebrafish
NP_499328    161 LFEQVATRLT 170 Caenorhabditis elegans
AAH72360     162 LFLDLACRLI 171 African clawed frog
NP_723242    159 LFYEIAAELI 168 fruit fly

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