1GT9,1GA4,3EDY,1SIO


Conserved Protein Domain Family
Peptidases_S53

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cd04056: Peptidases_S53 
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Peptidase domain in the S53 family
Members of the peptidases S53 (sedolisin) family include endopeptidases and exopeptidases sedolisin, kumamolysin, and (PSCP) Pepstatin-insensitive Carboxyl Proteinase. The S53 family contains a catalytic triad Glu/Asp/Ser with an additional acidic residue Asp in the oxyanion hole, similar to that of Asn in subtilisin. The stability of these enzymes may be enhanced by calcium, some members have been shown to bind up to 4 ions via binding sites with different affinity. Some members of this clan contain disulfide bonds. These enzymes can be intra- and extracellular, some function at extreme temperatures and pH values. Characterized sedolisins include Kumamolisin, an extracellular calcium-dependent thermostable endopeptidase from Bacillus. The enzyme is synthesized with a 188 amino acid N-terminal preprotein region which is cleaved after the extraction into the extracellular space with low pH. One kumamolysin paralog, kumamolisin-As, is believed to be a collagenase. TPP1 is a serine protease that functions as a tripeptidyl exopeptidase as well as an endopeptidase. Less is known about PSCP from Pseudomonas which is thought to be an aspartic proteinase.
Statistics
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PSSM-Id: 173788
View PSSM: cd04056
Aligned: 149 rows
Threshold Bit Score: 179.049
Threshold Setting Gi: 158422604
Created: 4-Oct-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
1GT9_1          5 AYTPLDVAQAYQFpe---------------glDGQGQCIAIIELGggy-----------------deTSLAQYFAslgv- 51   Bacillus sp. ...
1GA4_A          7 GHNPTEFPTIYDAss---------------apTAANTTVGIITIGgvs-----------------qtLQDLQQFTsang- 53   Pseudomonas sp.
YP_001523896  325 NPTPTQVAEAYNFp-----------------lTSKGVVTTPIGLVeg------------------gvPANLETALnayrt 369  Azorhizobium ...
XP_001748177  479 GLDAAALREAFEVdsqaa---------achrrNRGGVAVLSASEMysydglyry-----laannlplNAVLRTRDvtqt- 543  Monosiga brev...
XP_958412     212 GTFPGCIRKLYNLtytps----------pdfrSPSPVRFGIASFLeqy-----------------itHRDVTSFLatyar 264  Neurospora cr...
XP_001908980  295 ATSPRCIKQLYNItyppy---------npatsPRSPINFGVAGFLeqw-----------------ilHADVAYFLsqyqp 348  Podospora ans...
XP_961957      43 YSLPSRVARYIDYvlpapdpdpvssapksvavQDPPTLKGVIGARqtrdvdclqyiapqclrqlawlAEDLDMFFg---- 118  Neurospora cr...
YP_001524608  242 ELYPQDIAALYNFpls--------------gpAHQTGTLALIEIGigdal------------nptipGPSFQSRLqayl- 294  Azorhizobium ...
NP_378617     224 VISPLTIQQYFNFtl---------------ayEHGLNGKGSDIAIegvp---------------ecyVNVSDIYAfwkly 273  Sulfolobus to...
YP_001190414  226 IFTPNTIQQYFNFssly-----------aqgkTGSGSTIAIEGVPecy-----------------vnTTDIYSFWrlfn- 276  Metallosphaer...
Feature 1                                                                  #   #                  
1GT9_1         52 ----------------------------sapQVVSVSVdgatnqp---tgdpngPDGEVELDIEVAGAlap--gaKIAVY 98   Bacillus sp. ...
1GA4_A         54 ---------------------------lasvNTQTIQTgssngd----ysddqqGQGEWDLDSQSIVGsaggavqQLLFY 102  Pseudomonas sp.
YP_001523896  370 sv-----------------------lglpayTGSQFQIisgana-----rigvgDPGEESLDVSIIAGaap--nsQQLYY 419  Azorhizobium ...
XP_001748177  544 ---------------------------dlnpSFSYPYVlglhnaa---pndyrtAGDETMLDLAMMAAfap--gaYLDQI 591  Monosiga brev...
XP_958412     265 ellplrptpsrggsggsltlppvtnttseppYNITITLlnnatrwdphstdpalSGLEANLDVQYALSlgh--ptRVIYY 342  Neurospora cr...
XP_001908980  349 dyly-------------------rsatptgpYNFTVELingginp---qndpanAGIEASLDVEYAMAlgy--ptNVIYY 404  Podospora ans...
XP_961957     119 -------------------------------DFAPDLLt--------------nFNLEPNLDYKYTMAmak--piPVTNI 151  Neurospora cr...
YP_001524608  295 ---------------------------atagVPGSGAYyvqnsan---erynadNADERSLDVGVVSAivp--nsLIGLY 342  Azorhizobium ...
NP_378617     274 ---------------------------niprTGSLEVImfgnd-------tsggQSAENELDAEWSGAfsp--gaNVTIV 317  Sulfolobus to...
YP_001190414  277 ---------------------------iprtGSFNVITlgnd--------tsggQSGENELDAEYSGAfap--gaNIAIV 319  Metallosphaer...
Feature 1                                                          ####                           
1GT9_1         99 FAPnt------------------daGFLNAITTAVhdpt--hkpSIVSISWGGPedswa---pasiAAMNRAFLDAAAlg 155  Bacillus sp. ...
1GA4_A        103 MADqsas---------------gntGLTQAFNQAVsdn----vaKVINVSLGWCeadan--adgtlQAEDRIFATAAAqg 161  Pseudomonas sp.
YP_001523896  420 AYTg--------------------nQTFVGYQNAIfdsv--nnpSVLSSSFTDQqrfsp--nspfaSAYQGLFVDAALkn 475  Azorhizobium ...
XP_001748177  592 VTGmvdinttv-------dpllttaEVFLATLAMQedl----rpTVLSASYTLTlgdn----avvrHYLDSVAQALALvg 656  Monosiga brev...
XP_958412     343 ATGgrgtkldssgrplptndprannEPFLEFLQALlalpdnqipHVLSISYADDeqsvp---rkyaHRVCDLFAAVAArg 419  Neurospora cr...
XP_001908980  405 VTGgr------------------gtKLDRDGNPLLprrnrqralPRISVPRPYA------------LRVCDLFAALAArg 454  Podospora ans...
XP_961957     152 QVGdfv-----------------vqGNMNIMLAAF---------NAHYCRTGLD------------PQFDPVYPNPAP-- 191  Neurospora cr...
YP_001524608  343 VGSg--------------------dTVYTAYQTAIwdqq--nnpAVISSSWSDGlsfap--gspfaTAYRELFVDAALrg 398  Azorhizobium ...
NP_378617     318 FSDgyvgg------------vnlvgNLLNYWYEYYymvn-ylypNVISISVSVPesylaayypamlDLIHNMMLQAAAeg 384  Sulfolobus to...
YP_001190414  320 FSDgyvgg-------------kalvGNLLNYYYEYyymanylnpDVVSISVSLPesylaayypamlYMIHNIMVQLSVqg 386  Metallosphaer...
Feature 1              # ##                                                                       
1GT9_1        156 VTVLAAAGDSGStdgeqdg---------------lyhvdfpAASPYVLACGGTRLVASagrieret-------------- 206  Bacillus sp. ...
1GA4_A        162 QTFSVSSGDEGVyecnnrgypd----------gstysvswpASSPNVIAVGGTTLYTTsagaysnetvw----------- 220  Pseudomonas sp.
YP_001523896  476 MSVFLSSGDGGSggeygag---------------tamgrpsHSSPYAVIVGGASISNLqtasgdptlsslytagmtndpv 540  Azorhizobium ...
XP_001748177  657 VSVVLSSGDDGVymdgdasspn----------dycgqpsvsPLTAYGLVVGGVQYLPEsgdavac--------------- 711  Monosiga brev...
XP_958412     420 TSVLVATGDGGAagigfsagggdtcikndgsgrrafvptfpASCPWVTSVGATDNTALnlt------------------- 480  Neurospora cr...
XP_001908980  455 VSTFVASGDGGAag--------------------------tGQTRCVMNDGSGRKKMFiptfp----------------- 491  Podospora ans...
XP_961957     192 GGYNASDCGTHVpprviaimyawnk---awysdadfasifpASDPWVTSVGGTQFLPVvsngsssttassgmpsssssss 268  Neurospora cr...
YP_001524608  399 ISVFNDAFDGGSgnetgtg---------------ltnlftgSMSSYAMVVGGTSVSTLaaaqtdstlagvvlaaqqgdla 463  Azorhizobium ...
NP_378617     385 ISVLAASGDWGFesdhpppnfr---------igtyntiwypESDPYVTSVGGIFINASsngsiv---------------- 439  Sulfolobus to...
YP_001190414  387 TSVLAASGDWGFesnhpppnfh---------igvyntiwypESDPLVTSVGGIFLNATstgqiy---------------- 441  Metallosphaer...
Feature 1                                                                                         
1GT9_1        207 ---------------------------------------vwndgpdgGSTGGGVSrifplpswqera------------- 234  Bacillus sp. ...
1GA4_A        221 -------------------------------------negldsngklWATGGGYSvyeskpswq---------------- 247  Pseudomonas sp.
YP_001523896  541 tlmaltasgmkvlpqhmtaetysifvqtvwntysvngesinssyasnSASTGGINagslipsyqtdf------------- 607  Azorhizobium ...
XP_001748177  712 ----------------------------------------sletgssITSSGGFDqfatrpayqddpvsty--------- 742  Monosiga brev...
XP_958412     481 ---------------------------------------------gaAFSSGGFSeyfdrplwqraavdpyvssllrsrs 515  Neurospora cr...
XP_001908980  492 ------------------------------------------ascpyDFSAGGFSnyfdrpswqdeavkpyv-------- 521  Podospora ans...
XP_961957     269 ss---------------------ssssssssssslfpgetalddnntGSSGGSFSrlfpgpwyqgnltreyla------- 320  Neurospora cr...
YP_001524608  464 tlwqmvrgglta--wpvgagqlepfietvwnqyvlsgsrlfpgygenFATSGGVDttqatpgyqtafg------------ 529  Azorhizobium ...
NP_378617     440 ------------------------------------------sfsgwDYSTGGYSvvfpaqyyel--------------- 462  Sulfolobus to...
YP_001190414  442 ------------------------------------------sfsgwDYSTGGNSvvfpvqiyel--------------- 464  Metallosphaer...
Feature 1                                                                 #                       
1GT9_1        235 --------nvppsanpgagsGRGVPDVAGnadpaTGYEVVid-----gETTVIGGTSAVAPLFAALVARINQKLg----k 297  Bacillus sp. ...
1GA4_A        248 ------------svvsgtpgRRLLPDISFdaaqgTGALIYny-----gQLQQIGGTSLASPIFVGLWARLQSANs----n 306  Pseudomonas sp.
YP_001523896  608 ---------gltptnaqgetGRGLPDVSAlaggnSKYSIL--------NGGASAGTSAATPLWAALTAQIDTIFkdqglp 670  Azorhizobium ...
XP_001748177  743 ----lekvapvdhmqaslagNRAFPDVAGp---aHNIEVYgr-----eHRTLMAGTSASAPMLAGLLVLVNDVLlqqdlp 810  Monosiga brev...
XP_958412     516 skpgqpsqprdlkgvyfshnGRGMPDMAAi---gSGFQIIhr-----gEMVEVRGTSASTPVVAAMVALVNDQRlrqgkr 587  Neurospora cr...
XP_001908980  522 ---dgfvsrgdprvglfnstGRAVPDISAi---gSGFQIImg-----gEMSEVLGTSASAPVVAAMVALINDARmragkq 590  Podospora ans...
XP_961957     321 ---sapgaaelarqgyfngsGRGYPDISAm---aRSFLVAlh-----gGYHAVSGTSASTPVVAAMVAKINDARlhagks 389  Neurospora cr...
YP_001524608  530 --------ltptdadpshgvGRGAPDVSAlsqgnMGYLLPgsn---mlGVYPNGGTSAATPFWAALATEFNAIFkdqglp 598  Azorhizobium ...
NP_378617     463 ----------tslipftptyARTYPDIAFvs--aGGYNIPefgfglplIFEGQLFLWYGTSGAAPMTAAMVSLPg----v 526  Sulfolobus to...
YP_001190414  465 ----------tslipftpitVRTYPDIAFvs--aGGYNIPefgfglplIFDGQLFVWYGTSGAAPMTAAMVSLVg----q 528  Metallosphaer...
Feature 1                                                                                         
1GT9_1        298 PVGYLNPTLYQLpp--------------evFHDITEGNndia-------------------------------------- 325  Bacillus sp. ...
1GA4_A        307 SLGFPAASFYSAisst-----------pslVHDVKSGNngy--------------------------------------- 336  Pseudomonas sp.
YP_001523896  671 QLGYFNDLLYQAaaia-----------pgsFSDVTIGNntstyylytgteadk--------------------------- 712  Azorhizobium ...
XP_001748177  811 PLGDVTASIYAVarah-----------pevFQDVTRGSnrcgs------------------------------------- 842  Monosiga brev...
XP_958412     588 SLGWLNGHLYLDprv------------rrvLTDVKWGRsegcvfpgealeegrgkgkekywrhsvvekrqgnseedggth 655  Neurospora cr...
XP_001908980  591 SLGWLNPLLYSAkvr-------------avLRDVMVGEsygclf------------------------------------ 621  Podospora ans...
XP_961957     390 TVGFLNPVLYSAaagk-----------agvLRDVPLGKnhdc-------------------------------------- 420  Neurospora cr...
YP_001524608  599 NLGYSNDLYYIAaaia-----------pasFNDITAGNnvstfipgstyt------------------------------ 637  Azorhizobium ...
NP_378617     527 RFGALNYALYHIsyng----------ivvsPIGTFQGLvawip------------------------------------- 559  Sulfolobus to...
YP_001190414  529 RLGPLNYALYHIsysgevvtphgiikglsaWIPVTSGN------------------------------------------ 566  Metallosphaer...
Feature 1                                                                 
1GT9_1        326 -----------------------------nraRIYQAGPGWDPCTGLGSpigIRLL 352  Bacillus sp. MN-32
1GA4_A        337 ------------------------------ggYGYNAGTGWDYPTGWGSldiAKLS 362  Pseudomonas sp.
YP_001523896  713 -----------------pyvltqsgtkvvptgLGYPASAGYDQATGLGTpngLLLA 751  Azorhizobium caulinodans ORS 571
XP_001748177  843 ---------------------------svcceHGYPAKPGWDAVTGLGMprlPLLA 871  Monosiga brevicollis MX1
XP_958412     656 ggdgegkadeedwggegevgegegdqsenvilGGWDARKGWDPVTGLGVpgdFQEM 711  Neurospora crassa OR74A
XP_001908980  622 --------------------------pdgstqDGWPAVQGYDCVTGLGAvrqFDEL 651  Podospora anserina DSM 980
XP_961957     421 -----------------------------gvgEAFPARRAWDAVTGLGTpdfEKLK 447  Neurospora crassa OR74A
YP_001524608  638 ---------------------spqlggisatgLGYAAGQGYDLTTGLGSpngLLLA 672  Azorhizobium caulinodans ORS 571
NP_378617     560 ---------------------------vtsgnNPTPAHYGWNYVTGPGTydaYAMV 588  Sulfolobus tokodaii str. 7
YP_001190414  567 --------------------------------NPMPAHYGWNYVTGPGTydaYGMV 590  Metallosphaera sedula DSM 5348

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