Conserved Protein Domain Family
C2_ArfGAP

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cd04038: C2_ArfGAP 
C2 domain present in Arf GTPase Activating Proteins (GAP)
ArfGAP is a GTPase activating protein which regulates the ADP ribosylation factor Arf, a member of the Ras superfamily of GTP-binding proteins. The GTP-bound form of Arf is involved in Golgi morphology and is involved in recruiting coat proteins. ArfGAP is responsible for the GDP-bound form of Arf which is necessary for uncoating the membrane and allowing the Golgi to fuse with an acceptor compartment. These proteins contain an N-terminal ArfGAP domain containing the characteristic zinc finger motif (Cys-x2-Cys-x(16,17)-x2-Cys) and C-terminal C2 domain. C2 domains were first identified in Protein Kinase C (PKC). C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions.
Statistics
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PSSM-Id: 176003
View PSSM: cd04038
Aligned: 11 rows
Threshold Bit Score: 203.712
Threshold Setting Gi: 22093726
Created: 16-May-2006
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
putative Ca2+
Feature 1:putative Ca2+ binding pocket [ion binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #      #                                              # #     #
AAM62594       6 LGILRVHVKRGINLAIRDa--tTSDPYVVITLANQKLKTRVINNNCNPVWNEQLTLSIkdv-ndPIRLTVFDKDrFSGDD 82  thale cress
1808694       10 IGKLNVRVVRGSNLAICDplthTSDPYVVLHYGAQKVKTSVQKKNPNPVWNEVLQLSVtnp-tkPVHLEVFDEDkFTADD 88  Sporobolus stap...
AAF21204     216 VGLIKVNVVKGTNLAVRDv--mTSDPYVILALGQQSVKTRVIKNNLNPVWNETLMLSIpep-mpPLKVLVYDKDtFSTDD 292 thale cress
BAC07019      12 VGVVKVKVVRGTNLAVRDv--fSSDPYVVLKLGNQEVRTRTVRKNTNPVWNEDLTLIVqdlnhlLVTLEVYDRD-PFVDD 88  Japanese rice
ABR16527     213 LGLLKIKVVKGTNLAVRDm--vTSDPYVVLTIGHQTVKTRVIKSNLNPVWNEELMLSVpnp-mpPLKVKVFDKDtFSSDD 289 Sitka spruce
ABK23084       6 IGLLKVAVIRGTNLVATNfmnnSTDPYVVVSLGNQTVKTRTVKRNLNPEWDDELTVGVpsp-taQLKVEVMDKDiFSKDE 84  Sitka spruce
AAM64708       5 LGLLQVTVIQGKKLVIRDf--kSSDPYVIVKLGNESAKTKVINNCLNPVWNEELNFTLkdp-aaVLALEVFDKDrFKADD 81  thale cress
XP_001758688 143 LGMLKVRIVRGTNLAVRDl--lSSDPYVVATLGAQTAKTKVVNRNLNPVWNEELMFSVpsp-pqPLKLQVFDHDvLSADD 219 Physcomitrella ...
NP_001062683  12 RGVLKVVVASGTNLAVRDf--tSSDPYVVVRLAAMNKKTKVINSCLNPVWNEEMSFSIeep-agVIKFEVFDWDrFKYDD 88  Japanese rice
EAZ03980       5 LGLLKVRVMRGLNLAICDplthSSDPYVVLRHGSQKVKSSIRYHSINPEWNEELTLSItnm-mlPVKIEVFDKDtFTKDD 83  Indian rice
EEC81393       5 PGFLSVRVLRGVNLVSRDa--gGSDPYVVLHLDNQKLKTGVVKKTTNPVWNEELTLAVrnp-etPIQLEVFDKDtFSKDD 81  Indian rice
Feature 1                                                                                   
AAM62594      83 KMGDAEIDFRPfleahqmel------dfqklpngcAIKRIRPGRTNCLAeessitwsnGKIMQEMILRLKNVECG 151 thale cress
1808694       89 SMGVAEINLTDiydaakld--------lkhaadgtRIKTIYPVGVNYLGgeshvmwkdGKVVQDLILKLTKTDSG 155 Sporobolus stapfianus
AAF21204     293 FMGEAEIDIQPlvsaakaye-------tssikepmQLGSWVASKENTLVsdgiilledGKVKQDISLRLQNVERG 360 thale cress
BAC07019      89 PMGAAFFELRPlveaaaass-------rrrtpsgvDSKEDGTAVVPRSGssvvwsaseGKAAQGLVLRLAGVESG 156 Japanese rice
ABR16527     290 SMGEADVDIEPlvsaakaym-------nagvfgtkQIGKWLATADNALAsdsiinlidGQVKQEVTLKLQNVESG 357 Sitka spruce
ABK23084      85 FLGEAIVDLEPfvtiarkfhvetmsnnmrdhkkvkEIGRVLTSQHSELVkdspifykgGKIQQKLHLKLNDIKSG 159 Sitka spruce
AAM64708      82 KMGHASLSLQPlisvarlrh------vvrvssgetTLRKVLPDPENCVSrestiscidGEVVQSVWLRLCAVESG 150 thale cress
XP_001758688 220 SMGEAAIDLEPlilaaqmhq------gmfeefgceQIGKWLATDDNALVkdsnievidRQIKQDVHLKLQNVERG 288 Physcomitrella paten...
NP_001062683  89 KMGHAFLELQPvaaaaklrr------alrlttgetKLRKVAASVDNCLMcdsfvtyadGEIVLDARLRLRDVESG 157 Japanese rice
EAZ03980      84 SMGDAEFGILDfveiakqd--------hshlgdgaVMKTIHPDKENCFAaeshitwkdGKVSQDIVLKLRNTDTG 150 Indian rice
EEC81393      82 QMGDAEFDIEAlmqivrmd--------lqdirsgtVVRTVRPGRQCCLAdeshivwenGQIVQDMLLKLRNVETG 148 Indian rice

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