2Q3X


Conserved Protein Domain Family
C2B_RIM1alpha

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cd04028: C2B_RIM1alpha 
Click on image for an interactive view with Cn3D
C2 domain second repeat contained in Rab3-interacting molecule (RIM) proteins
RIMs are believed to organize specialized sites of the plasma membrane called active zones. They also play a role in controlling neurotransmitter release, plasticity processes, as well as memory and learning. RIM contains an N-terminal zinc finger domain, a PDZ domain, and two C-terminal C2 domains (C2A, C2B). C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. Members here have a type-I topology and do not bind Ca2+.
Statistics
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PSSM-Id: 175994
View PSSM: cd04028
Aligned: 12 rows
Threshold Bit Score: 249.612
Threshold Setting Gi: 156389191
Created: 11-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
homodimer
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:homodimer interface [polypeptide binding site]
Evidence:
  • Structure:2Q3X; Rattus norvegicus Rim1alpha forms a homodimer, contacts calculated at 3.5A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                  #   ## #  #            
2Q3X_A          2 PGPAQLVGrqtlatpAXGDIQIGXEDKkgQLEVEVIRARSLTQKPgsKSTPAPYVKVYLLENGACIAKKKTRIARKTLDP 81   Norway rat
XP_794399     208 LGPSQLVGrqalgspWMGDIQLSLEDKrgNLEVEVIRARSLVAKPgvKVPPAPYVKVYLMDGKHCVAKLKTKIARKTLDP 287  purple urchin
AAH59440      118 LGPAQLVGrqtlatpPMGDIQIGMVEKkgALEVEVIRARGLVGKPgsKALPAPYVKVYLLENGACIAKKKTKVARKTLDP 197  zebrafish
NP_001096180 1349 LGPAQLVGrqtlatpAMGDIQIGMVDKkgQLEVEVIRARGLIQKPgsKSTPAPYVKVYLLENGACVAKKKTRIARKTLDP 1428 western clawe...
XP_002119826  391 LGPGQVVGrqtlaasSLGDLELSFYETrgQLEVQVIRARNLVPKAntKVLPAPYVKVYLMFGMQCISKKKTKIARRTLDP 470  Ciona intesti...
XP_002160597  315 LGPAQVVGrqvlgspLMGDVQLSLLDRrgVLDVEIIRAKSLIIKPdsKTLPAPYVKVYLLCGKKCVEKRKTKTSSKRTLD 394  green hydra
NP_741831    1402 LGPGQVVGrqvlaspVLGEIQIALMAGrsGIDVEIIKAKNLVVKPgvKVCPAPYVKVYLMEGKQCIAKAKTNAATKTTSP 1481 nematode
CAZ33674     1013 LGPGQLVGrqvlgmpCLGEIQLSFYDRkgHLEVEVIRARGLQQKNtaKPLPAPYVKLHLLEGKQSVEKLRTMYPPRRTLD 1092 Schistosoma m...
XP_002114090  124 LGHGQVVGphvttssSFGKLQLGLQYKngILEVEVIRAKELIARNtkSLPSTYAKCYILDASDKYIAKKKSKSVRKTLEP 203  Trichoplax ad...
XP_419884    1526 LGPAQLVGrqtlatpAMGDIQIGMVDKkgQLEVEVIRARGLTQKPgsKSTPAPYVKVYLLENGACIAKKKTRIARKTLDP 1605 chicken
NP_001014630 2743 LGPGQLVGrqvlgapSLGDIQLSLCHQkgCLEVEVIRARGLQQKAqsKMLPAPYVKVYLVSGKRCVDKMKTSSARRTLDP 2822 fruit fly
XP_001634875   18 LGPSQMVGrqvlaspSMGDIQLGLCYRkgALEVEVIRAKGLLPKPgtKILPAPYVKVYVMEGKKCIAKKKTRTTRRTLEP 97   starlet sea a...
Feature 1                         # # #                                            ## 
2Q3X_A         82 LYQQSLVFDes-pqGKVLQVIVWGDYGRxDHKCFXGVAQILLeeld-lssXVIGWYKLFppsslvDPT 147  Norway rat
XP_794399     288 LYQQTLKFIeddhsNKVLQITVWGDYGRlERKIFMGVAQILLseld-lshPVMGWYKLFntssvaDIH 354  purple urchin
AAH59440      198 LYQQQLSFEes-pgGKVLQIIVWGDYGRmDHKSFMGAAQILLddld-lsnMVIGWFKLFppsslvDPT 263  zebrafish
NP_001096180 1429 LYQQTLVFEes-pnGKVLQVIVWGDYGRmDHKCFMGVAQILLeeld-lssVVIGWYKLFppsslvDPT 1494 western clawed frog
XP_002119826  471 VFHQTMNFDpt-qyGHTVQVIVWGDYGRmDHKAFMGVVQVRLenld-lsqNTIGWYRLFpttsliDAA 536  Ciona intestinalis
XP_002160597  395 PHYAQHLTFvqnyqGKVLQIMVWGDYGRlDRKVFMGVCQILLddld-lsqLVVGWYKLFtttslvHPP 461  green hydra
NP_741831    1482 LFQQHLIFNds-pkKKTLQVTVLGDYGRmERKVFMGISQIRLedlelgsqPLIGWYKLFhssslaGTG 1548 nematode
CAZ33674     1093 PLYQQQLCFsmpyhGKILQISVWGEYGRmDKKVFLGMCEIVLddld-lktVVFGWYKLFgmiasnAQH 1159 Schistosoma mansoni
XP_002114090  204 VYQQKLQFPvs-itDKTLEVIIWGNYNLiDKKVFMGVAQIVLseed-lsnLIISWYKLFdssaanINN 269  Trichoplax adhaerens
XP_419884    1606 LYQQTLVFDes-pqGKVLQVIVWGDYGRmDHKCFMGVAQILLeeld-lssVVIGWYKLFppsslvDPT 1671 chicken
NP_001014630 2823 LYQQQLVFKqs-ytGCILQITVWGDYGRiEKKVFMGVAQIMLddln-lsnIVIGWYKLF------GTT 2882 fruit fly
XP_001634875   98 LYQQMLDFRvd-inGKTLQIIVWGDYGRmDRKVFMGVVQILLddld-lsnLCMGWYKLFstssmcDPP 163  starlet sea anemone

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