Conserved Protein Domain Family
C2B_RasA1_RasA4

?
cd04025: C2B_RasA1_RasA4 
C2 domain second repeat present in RasA1 and RasA4
RasA1 and RasA4 are GAP1s (GTPase activating protein 1s ), Ras-specific GAP members, which suppresses Ras function by enhancing the GTPase activity of Ras proteins resulting in the inactive GDP-bound form of Ras. In this way it can control cellular proliferation and differentiation. Both proteins contain two C2 domains, a Ras-GAP domain, a plextrin homology (PH)-like domain, and a Bruton's Tyrosine Kinase (BTK) zinc binding domain. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-I topology.
Statistics
?
PSSM-Id: 175991
View PSSM: cd04025
Aligned: 5 rows
Threshold Bit Score: 233.146
Threshold Setting Gi: 21903461
Created: 16-May-2006
Updated: 2-Oct-2020
Structure
?
Aligned Rows:
 
Ca2+ binding
Feature 1:Ca2+ binding pocket [ion binding site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                       #     #                                                   # #    
O43374       134 RLRCSVLEARDLAPKDRNGTSDPFVRVRYkgRTRETSIVKKSCYPRWNETFEFELQega-----meALCVEAWDWDLVSR 208 human
XP_415761    134 RLRCTVLEARDLAKKDRNGASDPFVCVSYngKTQESTVVKKSCYPRWNEAFEFELPdpp-----aeKLCVEVWDWDLVSK 208 chicken
O95294       134 CLRCHVLQARDLAPRDISGTSDPFARVFWgsQSLETSTIKKTRFPHWDEVLELREMpga-----psPLRVELWDWDMVGK 208 human
AAH80934     134 TLHCHVLEARDLAPRDISGTSDPFVRIFCnnQTLETSVIKRTRFPRWNEVLEFDLRgieeldpsdqMISIEVWDWDMVGK 213 western clawed ...
NP_001092921 135 KLCCQVLEARDLAKKDRNGASDPFVRVRYngKTYESSVVKKSCYPRWNESFEFELDeal----tdsLLSVEVWDWDLVSR 210 zebrafish
Feature 1         #                                                
O43374       209 NDFLGKVVIDVQrlrvVQQEEGWFRLQPdq-skSRRHDEGNLGSLQLEVR 257 human
XP_415761    209 NDFLGKVVVSVQglqaAGHQEGWFRLQPdtakpREDRRRGSLGSLQLQVR 258 chicken
O95294       209 NDFLGMVEFSPKtl-qQKPPKGWFRLLPfp--rAEEDSGGNLGALRVKVR 255 human
AAH80934     214 NDFLGRVWFPIEplhkSPAVTSWFRLLPfg--nTDEENGGKLGSLRIKVS 261 western clawed frog
NP_001092921 211 NDFLGKVLFNINklqsALQEEGWFRLGPdk--sKHSEYEGTLGSLRLHLR 258 zebrafish

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap