Conserved Protein Domain Family
C2B_SLP_1-2-3-4

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cd04020: C2B_SLP_1-2-3-4 
C2 domain second repeat present in Synaptotagmin-like proteins 1-4
All Slp members basically share an N-terminal Slp homology domain (SHD) and C-terminal tandem C2 domains (named the C2A domain and the C2B domain) with the SHD and C2 domains being separated by a linker sequence of various length. Slp1/JFC1 and Slp2/exophilin 4 promote granule docking to the plasma membrane. Additionally, their C2A domains are both Ca2+ independent, unlike the case in Slp3 and Slp4/granuphilin in which their C2A domains are Ca2+ dependent. It is thought that SHD (except for the Slp4-SHD) functions as a specific Rab27A/B-binding domain. In addition to Slps, rabphilin, Noc2, and Munc13-4 also function as Rab27-binding proteins. It has been demonstrated that Slp3 and Slp4/granuphilin promote dense-core vesicle exocytosis. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin 1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions. This cd contains the second C2 repeat, C2B, and has a type-I topology.
Statistics
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PSSM-Id: 175987
View PSSM: cd04020
Aligned: 24 rows
Threshold Bit Score: 176.359
Threshold Setting Gi: 221113744
Created: 17-May-2006
Updated: 2-Oct-2020
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
AAH77272      361 RGRLRVAVKYVPqgaegl-------------------------------glpptGELHVWIKEAQQLVphk------agn 403  African clawe...
Q4VX76        448 NGELTVRAKLVLpsrprklqea----------------------qegtdqpslhGQLCLVVLGAKNLPvrp------dgt 499  human
XP_002196139  401 SAELLVSTRLLVpaqyqnlqse-----------------------gnkdqgvlnCQLQVMLFGAKNLPmlrs-----agm 452  zebra finch
AAW25226      284 RGEIVLAIKLVPgetg-----------------------------------gnrGEIHVWIKSAKGLSastdgvasqrdn 328  Schistosoma j...
XP_002166631  193 KGQLRVALKYDN------------------------------------------NVLTFVVKDAVGLPqvnp-----dkl 225  green hydra
XP_002612599   22 LGEVHLPLDNWDwddrqarwyqlqqpvvpvrgsdtsrkgrrkgspdrssgeagmGELHVFVKEARNLMavra-----ngs 96   Florida lancelet
NP_001138056  937 RGDIVVGLKYIPpenikssffsrgssitgsssnlrkfggsiksvasksertskgGQLHVLVKEAKHLSpika-----ngt 1011 fruit fly
EEC16277     1360 KGDLVLALKYMPpdvthrt----------------------------srqgpirGSLHVLVKEARNLMatrs-----ngt 1406 black-legged ...
XP_002166610  565 VGELKIALMFEDqgllnekekkrhdeqe----------elfgskkkkkgkkkssGKINVHIKEACHLPaads-----dgf 629  green hydra
XP_002124517  243 NGDMSIALRYIQgre-------------------------------------kfGQLEIVVQGVRSLPtaa-------dk 278  Ciona intesti...
AAH77272      404 VSTFVKCFVLPDSSPSNIQRTRVIPHSLHPVFNHTMVYDGFsGEDLKEACVECIIWDQGKIg--sRPLGGIRLSTGKGss 481  African clawe...
Q4VX76        500 LNSFVKGCLTLPDQQKLRLKSPVLRKQACPQWKHSFVFSGVtPAQLRQSSLELTVWDQALFgmndRLLGGTRLGSKGDt- 578  human
XP_002196139  453 LNSFVKGCLILPDQAEVKQKSPVLKKEGCPQWKHLFIFDGVtPAQLQQSCLHLTVWHKSTFsssdQFLGGAKLGAKEF-- 530  zebra finch
AAW25226      329 VDPYVKIYMLPGKEKHTKQKTKVVRRNNNPEWNQAIIYRDIiLSSLNEIGIEVSVWDHDRFss-nDFLGGCRLNCGSLn- 406  Schistosoma j...
XP_002166631  226 INPFVRCYLLPDRTRKSKRKTTVQKKTQNPVWNEKLEFNQVsVEDLQSCVLEVTVWDHDSSs--hQFLAGMRLGLSHGd- 302  green hydra
XP_002612599   97 SDPFVKGYLLPDKSKQSKQKTPYIKRNCNPTWNHTFVYKAVnPEELEQRCLELTVWDYDRMts-nDFLGGVRLSLGKEkp 175  Florida lancelet
NP_001138056 1012 CDAFCKSYLLPDRTRSSKQKTPVVKRTLHPSWNYTFVYEDVsLEELSERALELTVWDHDRLas-nEFVGGIRFSLGTGrs 1090 fruit fly
EEC16277     1407 SDPFCKSYLLPDRTKGSKQKTPVVKKSCNPKWNHTFVYPDVsLEELKDRCLELTVWDYDKIts-nDFLGGVRLGLGTGkl 1485 black-legged ...
XP_002166610  630 SDPFCKCYLLPLKSSKTKKKTPIIRRTVNPVWDFQLSYELD-YEDLSDFGIEFTIWDWDRAk--nDFLGCTRINLGGKm- 705  green hydra
XP_002124517  279 SNIFVKGYFLPDKARATKQKTPLLQSSSNVQFDHQFVYTKVaSDELSQRCVEITVWKEGLIgp-nKLLGAVRLSTGTGqs 357  Ciona intesti...
AAH77272      482 y---ensVNWMDStEEEKAFWKSVINNPAEWAEIVLPLR 517  African clawed frog
Q4VX76        579 ------aVGGDAC-SLSKLQWQKVLSSPNLWTDMTLVLH 610  human
XP_002196139  531 -------FGPMDLaSQGVLQWQEVLHNPNTWMDFTLVLH 562  zebra finch
AAW25226      407 -------QPWMDAtNGEKSAWLAMCERPNIWIEATIQLR 438  Schistosoma japonicum
XP_002166631  303 -------EYWHDCiDKEVLIWESMMRHRGIFAEFTIPLR 334  green hydra
XP_002612599  176 cnrlgqpIEWMDSqGEEITAWRTMLNKPNVWVDSCLLLR 214  Florida lancelet
NP_001138056 1091 y---grqVEWMDAtGKELSLWQNMLDRPNFWVEGSLVLR 1126 fruit fly
EEC16277     1486 y---grdVDWMDSqGEELTLWRSMLERPNLWIDGSLLLR 1521 black-legged tick
XP_002166610  706 -------GNWDDAeGVEILAWQHLIDQPNKWKDFVIPLR 737  green hydra
XP_002124517  358 f---gdnVHWMDStTEEIQLWQKMFDNFGSWSEGTITLR 393  Ciona intestinalis
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